ID R6KHX4_9BACE Unreviewed; 1050 AA.
AC R6KHX4;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 40.
DE RecName: Full=beta-galactosidase {ECO:0000256|ARBA:ARBA00012756};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756};
DE AltName: Full=Lactase {ECO:0000256|ARBA:ARBA00032230};
GN ORFNames=BN506_00587 {ECO:0000313|EMBL:CDB73050.1};
OS Bacteroides cellulosilyticus CAG:158.
OC Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
OC Bacteroides.
OX NCBI_TaxID=1263038 {ECO:0000313|EMBL:CDB73050.1, ECO:0000313|Proteomes:UP000018191};
RN [1] {ECO:0000313|EMBL:CDB73050.1, ECO:0000313|Proteomes:UP000018191}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:158 {ECO:0000313|Proteomes:UP000018191};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412};
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family.
CC {ECO:0000256|ARBA:ARBA00007401}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CDB73050.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CBEB010000390; CDB73050.1; -; Genomic_DNA.
DR AlphaFoldDB; R6KHX4; -.
DR Proteomes; UP000018191; Unassembled WGS sequence.
DR GO; GO:0009341; C:beta-galactosidase complex; IEA:InterPro.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:1901575; P:organic substance catabolic process; IEA:UniProt.
DR Gene3D; 2.70.98.10; -; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR InterPro; IPR004199; B-gal_small/dom_5.
DR InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR014718; GH-type_carb-bd.
DR InterPro; IPR006101; Glyco_hydro_2.
DR InterPro; IPR006103; Glyco_hydro_2_cat.
DR InterPro; IPR006102; Glyco_hydro_2_Ig-like.
DR InterPro; IPR006104; Glyco_hydro_2_N.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR032312; LacZ_4.
DR PANTHER; PTHR46323; BETA-GALACTOSIDASE; 1.
DR PANTHER; PTHR46323:SF2; BETA-GALACTOSIDASE; 1.
DR Pfam; PF02929; Bgal_small_N; 1.
DR Pfam; PF00703; Glyco_hydro_2; 1.
DR Pfam; PF02836; Glyco_hydro_2_C; 1.
DR Pfam; PF02837; Glyco_hydro_2_N; 1.
DR Pfam; PF16353; LacZ_4; 1.
DR PRINTS; PR00132; GLHYDRLASE2.
DR SMART; SM01038; Bgal_small_N; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 2.
DR SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|ARBA:ARBA00022837};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Reference proteome {ECO:0000313|Proteomes:UP000018191};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..20
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 21..1050
FT /note="beta-galactosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5004423640"
FT DOMAIN 758..1033
FT /note="Beta galactosidase small chain/"
FT /evidence="ECO:0000259|SMART:SM01038"
SQ SEQUENCE 1050 AA; 120547 MW; 9B988CAD3FFD9377 CRC64;
MKAKVYTLLF SSLLCVSALA DNEPWQNPQI NEINREPMHA HFIPFTNEAN ALKQRSLPAD
VRFDVNPATE RRVSLDGTWK FLFSKNNDLC PKDFHKPGYN TRKWSKIQVP GSWELQGFDA
PIYTDTRYPF PANPPYVPAD YNPVGAYIRE FTVPNGWEGM DIFLDFEGVE SAYYVWVNGE
LAGYAEDSRL PSHFNVTKLL KKGSNKLAVK VFRYSDGSYL EGQDYWKYSG IERDVYLYAR
PQSRVRDFRM TAELVNDYKD RELDLDILLH QPKVGESVEV KVLDEGREIY NRKKSITFTT
DTLFSQQEIF PNIRAWNAET PNTYTLVVST FDTQGKPLES FTHLFGFRTV EMRNGMQMIN
GKAVLFKGVN RHEHDPHKGR TISVASMIHD IQLMKQFNLN GVRNCHYPNY SAWYELCTEF
GLYMVDEANI ESHGMMDHKD GTLANYPDWE RPFMERMSRM IARDRNYSAI VTWSMGNESG
YGKHFEALYD YTKKIDPTRP VQYEGGGYDA KSDIYCPMYA RIWSLRRHIN QRDKRPLIMC
EYAHAMGNSV GNFQDYWDLI YKYDQLQGGF IWDWVDQTFA IKDKNNRDIW AFGGDMGFVG
IVNDSNFCAN GLVAADRSLH PHIYEVKKVL QYIHFEPVAF TPNKIKVSNR HDFIGLEGYT
LRWAVECDGK AVQGGEMNFP VITPGSSANI ELPLKTLPAD GKEYFLTLRA FTKHEAPLIP
KGHEVAIEQW ILPTTQITGK VQPANSTLAT DRNNEAITLK GNNFQIAFST QNGEMTELIY
HGKNLIKEGL QPNFWRPLTD NDIPNGHLNR CATWKTAGKD AKLENLEVTE KQQIVIVTVT
YKMEAQGSTL QTIYDIHPDG AVRVSMHFTP GKQALNEMPR LGMRMILPAE YEMMSWLGRG
PQENYADRKT GALIGLYNVT VWEQFHPYVR AQETANHCDV RWIALRNAAG EGLLVTGEDP
LSVSAWNFPM EDIEYRPSQV ERRHGGSILK KDIIWLNIDH QQMGVGGDNT WGAQVHPEYT
ITPHEWKYSF TLQPLGAKDD AAEQAHKCWF
//
