ID R6QMJ2_9FIRM Unreviewed; 315 AA.
AC R6QMJ2;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=Glycoside hydrolase family 43 {ECO:0008006|Google:ProtNLM};
GN ORFNames=BN563_00225 {ECO:0000313|EMBL:CDC32066.1};
OS Eubacterium sp. CAG:251.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Eubacteriaceae;
OC Eubacterium.
OX NCBI_TaxID=1262886 {ECO:0000313|EMBL:CDC32066.1, ECO:0000313|Proteomes:UP000018414};
RN [1] {ECO:0000313|EMBL:CDC32066.1, ECO:0000313|Proteomes:UP000018414}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:251 {ECO:0000313|Proteomes:UP000018414};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CDC32066.1}.
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DR EMBL; CBFD010000002; CDC32066.1; -; Genomic_DNA.
DR AlphaFoldDB; R6QMJ2; -.
DR STRING; 1262886.BN563_00225; -.
DR Proteomes; UP000018414; Unassembled WGS sequence.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR CDD; cd08991; GH43_HoAraf43-like; 1.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR43772:SF2; BETA-1,4-XYLOSIDASE (EUROFUNG); 1.
DR PANTHER; PTHR43772; ENDO-1,4-BETA-XYLANASE; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361187};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361187};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00022651};
KW Reference proteome {ECO:0000313|Proteomes:UP000018414};
KW Xylan degradation {ECO:0000256|ARBA:ARBA00022651}.
FT ACT_SITE 16
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT ACT_SITE 195
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT SITE 124
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ SEQUENCE 315 AA; 36657 MW; EEE15F557B982E8B CRC64;
MHLLEINKEN YIGQADPFIF EANGKFYIYT TGSDGIYAYF ADDLFGKWNF YGRVFTYEGK
GVHDFWAPSV IEIDGTYYLY CSFEFFDDEP DQGGHHQAMH VSSSKSPLGP FENAKQLLHP
FSIDSHVVKN ENGLFIFYST NTFEGERPGT YIVCDRLLTP EKAEGKPVTM LTPTLDEDIY
MRDRYKKGEN WHTIEGAFYF KEGDWQYLMY SGNCYLQPTY YIGYARAKTD ETDLTKIKFE
KYPDENTYHP VMKANEFEEG TGHHSMIKYK GQWYAIYHAR DIEDDGLAGD RRNARICKLH
VNDGIITAET YKDHI
//
