ID R6RGU8_9FIRM Unreviewed; 645 AA.
AC R6RGU8;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 24-JAN-2024, entry version 40.
DE RecName: Full=Aconitate hydratase A {ECO:0000256|ARBA:ARBA00019378};
DE EC=4.2.1.3 {ECO:0000256|ARBA:ARBA00012926};
DE AltName: Full=Iron-responsive protein-like {ECO:0000256|ARBA:ARBA00031977};
DE AltName: Full=RNA-binding protein {ECO:0000256|ARBA:ARBA00031081};
GN ORFNames=BN563_01660 {ECO:0000313|EMBL:CDC33692.1};
OS Eubacterium sp. CAG:251.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Eubacteriaceae;
OC Eubacterium.
OX NCBI_TaxID=1262886 {ECO:0000313|EMBL:CDC33692.1, ECO:0000313|Proteomes:UP000018414};
RN [1] {ECO:0000313|EMBL:CDC33692.1, ECO:0000313|Proteomes:UP000018414}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:251 {ECO:0000313|Proteomes:UP000018414};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate = D-threo-isocitrate; Xref=Rhea:RHEA:10336,
CC ChEBI:CHEBI:15562, ChEBI:CHEBI:16947; EC=4.2.1.3;
CC Evidence={ECO:0000256|ARBA:ARBA00023501};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC from oxaloacetate: step 2/2. {ECO:0000256|ARBA:ARBA00004717}.
CC -!- SUBUNIT: Monomer. {ECO:0000256|ARBA:ARBA00011245}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CDC33692.1}.
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DR EMBL; CBFD010000085; CDC33692.1; -; Genomic_DNA.
DR AlphaFoldDB; R6RGU8; -.
DR STRING; 1262886.BN563_01660; -.
DR UniPathway; UPA00223; UER00718.
DR Proteomes; UP000018414; Unassembled WGS sequence.
DR GO; GO:0003994; F:aconitate hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR CDD; cd01585; AcnA_Bact; 1.
DR CDD; cd01579; AcnA_Bact_Swivel; 1.
DR Gene3D; 3.30.499.10; Aconitase, domain 3; 2.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR018136; Aconitase_4Fe-4S_BS.
DR InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR InterPro; IPR006250; Aconitase_put.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR NCBIfam; TIGR01342; acon_putative; 1.
DR PANTHER; PTHR43160; ACONITATE HYDRATASE B; 1.
DR PANTHER; PTHR43160:SF3; ACONITATE HYDRATASE, MITOCHONDRIAL; 1.
DR Pfam; PF00330; Aconitase; 2.
DR Pfam; PF00694; Aconitase_C; 1.
DR PRINTS; PR00415; ACONITASE.
DR SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
DR PROSITE; PS00450; ACONITASE_1; 1.
DR PROSITE; PS01244; ACONITASE_2; 1.
PE 4: Predicted;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000018414}.
FT DOMAIN 8..280
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
FT DOMAIN 280..407
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
FT DOMAIN 519..578
FT /note="Aconitase A/isopropylmalate dehydratase small
FT subunit swivel"
FT /evidence="ECO:0000259|Pfam:PF00694"
SQ SEQUENCE 645 AA; 69426 MW; A4E4F18162BFBE92 CRC64;
MGLTIAQKLI KAHLVEGEMK VGTEIGLRID QTLTQDATGT MAYLEFEAMG VDRVKTEKSV
AYIDHNTLQT GFENADDHRY IQSVTKKHGI YFSRPGNGIC HQVHLERFGI PGKTLIGSDS
HTPTGGGIGM LAMGAGGLDV AVAMGGGTYY IPMPKMTRIN LTGYLKPWVS AKDVILEVLR
IMSVKGGVGK IIEYGGEGVK TLSVPERATI TNMGAELGAT TSIFPSDEIT LAFLKAQGRE
NDWTEILPDE DAEYDEVIDI DLCSLTPMAA CPHMPDNVKT TDEIGKIKVD QVAIGSCTNS
SFVDMMKVAS ILKGKTIAPS VSLCIAPGSK QVLNMLALNG ALSDMISAGA RILESACGPC
IGMGQSPNSG GVSLRTFNRN FEGRSGTKDG QIYLVSPEVA AASALTGYLT DPRELGDAPK
ISMPEHFIVN DNMIEAPASP EDADKVEVLR GPNIKPFPKT EKLPEDITAK AVLKVGDNIT
TDHIMPAGAK ILPYRSNIPH LSQYCFAVCD ETFPERIKAE GKGFIVGGAN YGQGSSREHA
ALVPLYLGVK AVITKSFARI HVANLVNAGI LPFTFANEED YDKIDQMDEL SLPNIKDAIE
NNKPVVLKNL TKGEEYQLNS SHLSARQRAM LVCGGLLDYT REMNK
//
