ID   R6SCT5_9FIRM            Unreviewed;       497 AA.
AC   R6SCT5;
DT   24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT   24-JUL-2013, sequence version 1.
DT   27-MAR-2024, entry version 22.
DE   SubName: Full=Glycoside hydrolase family 43 YxiA {ECO:0000313|EMBL:CDC44417.1};
GN   ORFNames=BN652_00753 {ECO:0000313|EMBL:CDC44417.1};
OS   Firmicutes bacterium CAG:424.
OC   Bacteria; Bacillota.
OX   NCBI_TaxID=1263022 {ECO:0000313|EMBL:CDC44417.1, ECO:0000313|Proteomes:UP000018167};
RN   [1] {ECO:0000313|EMBL:CDC44417.1, ECO:0000313|Proteomes:UP000018167}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MGS:424 {ECO:0000313|Proteomes:UP000018167};
RA   Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA   Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA   Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA   Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA   Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA   Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA   Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA   Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA   Wang J., Brunak S., Ehrlich S.D.;
RT   "Dependencies among metagenomic species, viruses, plasmids and units of
RT   genetic variation.";
RL   Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC   -!- PATHWAY: Glycan metabolism; L-arabinan degradation.
CC       {ECO:0000256|ARBA:ARBA00004834}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC       {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:CDC44417.1}.
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DR   EMBL; CBFI010000044; CDC44417.1; -; Genomic_DNA.
DR   AlphaFoldDB; R6SCT5; -.
DR   Proteomes; UP000018167; Unassembled WGS sequence.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   Gene3D; 2.40.128.10; -; 1.
DR   InterPro; IPR032291; Abn2_C.
DR   InterPro; IPR006710; Glyco_hydro_43.
DR   InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR   PANTHER; PTHR43301; ARABINAN ENDO-1,5-ALPHA-L-ARABINOSIDASE; 1.
DR   PANTHER; PTHR43301:SF3; ARABINOSIDASE-RELATED; 1.
DR   Pfam; PF16369; GH43_C; 1.
DR   Pfam; PF04616; Glyco_hydro_43; 1.
DR   SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|RuleBase:RU361187};
KW   Hydrolase {ECO:0000256|RuleBase:RU361187, ECO:0000313|EMBL:CDC44417.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000018167}.
FT   DOMAIN          384..489
FT                   /note="Extracellular endo-alpha-(1->5)-L-arabinanase C-
FT                   terminal"
FT                   /evidence="ECO:0000259|Pfam:PF16369"
FT   ACT_SITE        52
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT   ACT_SITE        240
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT   SITE            188
FT                   /note="Important for catalytic activity, responsible for
FT                   pKa modulation of the active site Glu and correct
FT                   orientation of both the proton donor and substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ   SEQUENCE   497 AA;  55849 MW;  40FB4956A1F3C64D CRC64;
     MGHHEKGDKM KKRIYFLLGT ILLAGLAGCG QKEKLKAEKP VEKEFQEVSV HDPSVVQGDD
     GSYYIFGSHL AVAKTDDFMN WETVNQGVKD DNGVIPDVYL NMKEAFEWSH SNTFWAPDVA
     RLEDGKYHLY YCNCQGDAPV SCLGTAVSDK VDGPYVNEGL MLKSGMPASE KDEDGDLYQA
     TTDPNVVDPC TFYDAQGRLW MVYGSYSGGI FIKEMDAKTG LPLEEGYGKK LLGGNHLRIE
     APYIMYNEET GYYYMFLSFG GLDSDGGYNI RVCRSENPDG PYVDSMGQEM EDCKGAAGTA
     FSDATAALYG TKLMGSYKFL WKEGEEGENR EGYLSPGHNS CIYQEETGKY FMIYHTRFEN
     RGEEHEVRVH QMFFNEEGWP VIAPYRYTEE ELKEYPAKEL AGGYKLINHG REISAEMSES
     QEIQLTSKGE IQGLEEEGTW KIGDNCSIEL EIDGKTYKGI YLKQWDEDGK KYVMTFTALN
     SETGMSVWGS GLNAIEE
//