ID   R6V5F0_9FIRM            Unreviewed;       520 AA.
AC   R6V5F0;
DT   24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT   24-JUL-2013, sequence version 1.
DT   24-JAN-2024, entry version 22.
DE   SubName: Full=Beta-xylosidase {ECO:0000313|EMBL:CDC92260.1};
GN   ORFNames=BN546_01752 {ECO:0000313|EMBL:CDC92260.1};
OS   Firmicutes bacterium CAG:227.
OC   Bacteria; Bacillota.
OX   NCBI_TaxID=1263010 {ECO:0000313|EMBL:CDC92260.1, ECO:0000313|Proteomes:UP000018294};
RN   [1] {ECO:0000313|EMBL:CDC92260.1, ECO:0000313|Proteomes:UP000018294}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MGS:227 {ECO:0000313|Proteomes:UP000018294};
RA   Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA   Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA   Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA   Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA   Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA   Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA   Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA   Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA   Wang J., Brunak S., Ehrlich S.D.;
RT   "Dependencies among metagenomic species, viruses, plasmids and units of
RT   genetic variation.";
RL   Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC       {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:CDC92260.1}.
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DR   EMBL; CBGC010000122; CDC92260.1; -; Genomic_DNA.
DR   AlphaFoldDB; R6V5F0; -.
DR   STRING; 1263010.BN546_01752; -.
DR   Proteomes; UP000018294; Unassembled WGS sequence.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd18617; GH43_XynB-like; 1.
DR   Gene3D; 2.60.120.200; -; 1.
DR   InterPro; IPR013320; ConA-like_dom_sf.
DR   InterPro; IPR041542; GH43_C2.
DR   InterPro; IPR006710; Glyco_hydro_43.
DR   InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR   PANTHER; PTHR42812; BETA-XYLOSIDASE; 1.
DR   PANTHER; PTHR42812:SF12; BETA-XYLOSIDASE-RELATED; 1.
DR   Pfam; PF17851; GH43_C2; 1.
DR   Pfam; PF04616; Glyco_hydro_43; 1.
DR   SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR   SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361187};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361187};
KW   Reference proteome {ECO:0000313|Proteomes:UP000018294}.
FT   DOMAIN          324..516
FT                   /note="Beta-xylosidase C-terminal Concanavalin A-like"
FT                   /evidence="ECO:0000259|Pfam:PF17851"
FT   ACT_SITE        15
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT   ACT_SITE        185
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT   SITE            123
FT                   /note="Important for catalytic activity, responsible for
FT                   pKa modulation of the active site Glu and correct
FT                   orientation of both the proton donor and substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ   SEQUENCE   520 AA;  59655 MW;  50D6C39E07617745 CRC64;
     MVIAKNPILS GFYPDPSICR VGNDFYIVNS SFVYAPGVPI FHSRDLAHWE QIGNILESKE
     QLQVEKSEIS RGIFAPTIRY HEGLFYMITT NVSYGGNFIV TAKNPEGPWS EPYYLGEEAI
     GIDPSLFFDD DGRCYYCGTR PNPEGVRYNG DWEIWVQELD LKSMKLVGES MAIWKGAVKG
     VIWPEGPHIY KIGEYYYLMH AEGGTGPEHS ISVARSKKLF QWFEGCPRNP IFTHRNLGMD
     YPVVYAGHGD LVDDGYGNWY VVMLASRPCK KHSSMGRETF LAKVIWENGW PVIAPQVGRL
     EDFVEIPLEE HRFANEVTSS DCIQFWENEL DERLVGIEKR DEEIYSLSER KGMLRLYCRK
     EKISEKDYSS YLGLRQKSYR FEVETGIEFE PKAENETAGV ILYQNHENHL KFEIKQVEGK
     KVFEVNSYIH GEETKLSIVD ISKWQGILKI MIQCHDQEAR VWIGKEKNLK PVAEKIPLTA
     YTTEEAGGFV GCTVGMYASS NGKQSENYAD FGWFYLKNMD
//