ID   R6YIB7_9FIRM            Unreviewed;       549 AA.
AC   R6YIB7;
DT   24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT   24-JUL-2013, sequence version 1.
DT   27-MAR-2024, entry version 33.
DE   RecName: Full=Peptidase S8/S53 domain-containing protein {ECO:0000259|Pfam:PF00082};
GN   ORFNames=BN600_01933 {ECO:0000313|EMBL:CDD35703.1};
OS   Roseburia sp. CAG:309.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae; Roseburia.
OX   NCBI_TaxID=1262945 {ECO:0000313|EMBL:CDD35703.1, ECO:0000313|Proteomes:UP000018292};
RN   [1] {ECO:0000313|EMBL:CDD35703.1, ECO:0000313|Proteomes:UP000018292}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MGS:309 {ECO:0000313|Proteomes:UP000018292};
RA   Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA   Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA   Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA   Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA   Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA   Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA   Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA   Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA   Wang J., Brunak S., Ehrlich S.D.;
RT   "Dependencies among metagenomic species, viruses, plasmids and units of
RT   genetic variation.";
RL   Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the peptidase S8 family. {ECO:0000256|PROSITE-
CC       ProRule:PRU01240}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:CDD35703.1}.
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DR   EMBL; CBGX010000252; CDD35703.1; -; Genomic_DNA.
DR   AlphaFoldDB; R6YIB7; -.
DR   STRING; 1262945.BN600_01933; -.
DR   Proteomes; UP000018292; Unassembled WGS sequence.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd07478; Peptidases_S8_CspA-like; 1.
DR   Gene3D; 2.60.120.1290; -; 1.
DR   Gene3D; 3.40.50.200; Peptidase S8/S53 domain; 1.
DR   InterPro; IPR034045; Pep_S8_CspA-like.
DR   InterPro; IPR017310; Pept_S8A_subtilisin_clostridia.
DR   InterPro; IPR000209; Peptidase_S8/S53_dom.
DR   InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR   InterPro; IPR023827; Peptidase_S8_Asp-AS.
DR   InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR   PANTHER; PTHR43399:SF5; CELL WALL-ASSOCIATED PROTEASE; 1.
DR   PANTHER; PTHR43399; SUBTILISIN-RELATED; 1.
DR   Pfam; PF00082; Peptidase_S8; 2.
DR   PIRSF; PIRSF037894; Subtilisin_rel_CspABC; 1.
DR   PRINTS; PR00723; SUBTILISIN.
DR   SUPFAM; SSF52743; Subtilisin-like; 1.
DR   PROSITE; PS51892; SUBTILASE; 1.
DR   PROSITE; PS00136; SUBTILASE_ASP; 1.
PE   3: Inferred from homology;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW   ProRule:PRU01240};
KW   Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|PROSITE-
KW   ProRule:PRU01240}; Reference proteome {ECO:0000313|Proteomes:UP000018292};
KW   Serine protease {ECO:0000256|ARBA:ARBA00022825, ECO:0000256|PROSITE-
KW   ProRule:PRU01240}.
FT   DOMAIN          95..287
FT                   /note="Peptidase S8/S53"
FT                   /evidence="ECO:0000259|Pfam:PF00082"
FT   DOMAIN          420..538
FT                   /note="Peptidase S8/S53"
FT                   /evidence="ECO:0000259|Pfam:PF00082"
FT   ACT_SITE        104
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR615500-1,
FT                   ECO:0000256|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        171
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR615500-1,
FT                   ECO:0000256|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        488
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR615500-1,
FT                   ECO:0000256|PROSITE-ProRule:PRU01240"
SQ   SEQUENCE   549 AA;  60462 MW;  02BEDE6DCCE7640E CRC64;
     MTCTEAIYSE QVLDYIVSSY IREETLVERY RPTCYSRISS GGTIVYREES RIDSQTINQF
     GYGVVPHVYG LMGYEALEAA GVMQIRRQPY LDLYGQGVII GFVDTGIDFT HEAFLNADGT
     TRLHSIWDQT IREGGPSQFD YGRVFSEEEI NAALKQEQSS SYLPTRDEIG HGTFLAGVAA
     GNQIPGREFS GVAPLSQIIA VKCKEAKNSY REYYRVPSKV PCYQENDIMA GIMYILQVAY
     SLGRPAAICV GMGSSLGNHD GASPLCHLID INNMAGASIV ACAGNEGAAG HHYEITRKEE
     EIQIDVQRAS LGFVCELWWQ TPGSVSFDVI SPGGSSLGLT RAEDGLQRQK RFPVENTNLE
     IRSGRLQEQT RDQVILFRFE DTKPGVWKII VNSRQDNPRY HMWLPISQFL EGETVFTRPN
     PDTTICEPGN ARNAITISAY NPVNNAFYAQ ASRGFTPNGI IKPDVTAPGV EIFGPYPRGQ
     YGTMTGTSVA AAVTTGIAAL FLQEFDVADL NGNIVPELLI RGASPHGGEY PSQEWGYGTV
     DAYESITFE
//