ID R7CAJ9_9CLOT Unreviewed; 1519 AA.
AC R7CAJ9;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE SubName: Full=Glutamate synthase domain 2 {ECO:0000313|EMBL:CDD74980.1};
GN ORFNames=BN737_01195 {ECO:0000313|EMBL:CDD74980.1};
OS Clostridium sp. CAG:62.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=1262828 {ECO:0000313|EMBL:CDD74980.1, ECO:0000313|Proteomes:UP000018137};
RN [1] {ECO:0000313|EMBL:CDD74980.1, ECO:0000313|Proteomes:UP000018137}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:62 {ECO:0000313|Proteomes:UP000018137};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974};
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000256|ARBA:ARBA00001917};
CC -!- COFACTOR:
CC Name=[3Fe-4S] cluster; Xref=ChEBI:CHEBI:21137;
CC Evidence={ECO:0000256|ARBA:ARBA00001927};
CC -!- PATHWAY: Amino-acid biosynthesis. {ECO:0000256|ARBA:ARBA00029440}.
CC -!- SIMILARITY: Belongs to the glutamate synthase family.
CC {ECO:0000256|ARBA:ARBA00009716}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CDD74980.1}.
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DR EMBL; CBHQ010000121; CDD74980.1; -; Genomic_DNA.
DR Proteomes; UP000018137; Unassembled WGS sequence.
DR GO; GO:0051538; F:3 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0015930; F:glutamate synthase activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006537; P:glutamate biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR CDD; cd00982; gltB_C; 1.
DR CDD; cd00713; GltS; 1.
DR CDD; cd02808; GltS_FMN; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 2.
DR Gene3D; 2.160.20.60; Glutamate synthase, alpha subunit, C-terminal domain; 1.
DR Gene3D; 3.60.20.10; Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR017932; GATase_2_dom.
DR InterPro; IPR002489; Glu_synth_asu_C.
DR InterPro; IPR036485; Glu_synth_asu_C_sf.
DR InterPro; IPR006982; Glu_synth_centr_N.
DR InterPro; IPR002932; Glu_synthdom.
DR InterPro; IPR029055; Ntn_hydrolases_N.
DR PANTHER; PTHR11938; FAD NADPH DEHYDROGENASE/OXIDOREDUCTASE; 1.
DR PANTHER; PTHR11938:SF133; GLUTAMATE SYNTHASE (NADH); 1.
DR Pfam; PF00310; GATase_2; 1.
DR Pfam; PF04898; Glu_syn_central; 1.
DR Pfam; PF01645; Glu_synthase; 1.
DR Pfam; PF01493; GXGXG; 1.
DR SUPFAM; SSF69336; Alpha subunit of glutamate synthase, C-terminal domain; 1.
DR SUPFAM; SSF51395; FMN-linked oxidoreductases; 1.
DR SUPFAM; SSF56235; N-terminal nucleophile aminohydrolases (Ntn hydrolases); 1.
DR PROSITE; PS51278; GATASE_TYPE_2; 1.
PE 3: Inferred from homology;
KW 3Fe-4S {ECO:0000256|ARBA:ARBA00023291};
KW Amino-acid biosynthesis {ECO:0000256|ARBA:ARBA00022605};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW FMN {ECO:0000256|ARBA:ARBA00022643};
KW Glutamate biosynthesis {ECO:0000256|ARBA:ARBA00023164};
KW Glutamine amidotransferase {ECO:0000256|ARBA:ARBA00022962};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000018137}.
FT DOMAIN 25..416
FT /note="Glutamine amidotransferase type-2"
FT /evidence="ECO:0000259|PROSITE:PS51278"
SQ SEQUENCE 1519 AA; 168824 MW; 885B46D7DBEF0785 CRC64;
MSEYVEECKR KPIGLYDPRF EHDNCGIGAI VNIKGKKTHS TVKNALEIVA NLEHRAGKDG
EGKTGDGVGI LLQISHKFFS KVAEKLGIDI GGEREYGVGM FFFPQDELKR NQAKKMFENI
IAKEGMEFLA WREVPIAPDI LGKRAVECMP YIMQCFVKKP KKVEKGLDFD RKLYIARRVF
EQSNENTYVV SLSSRTIVYK GMFLVSQLRL FFDDLQDVDY ESAIAIVHSR FSTNTQPSWQ
RAHPNRFIVH NGEINTILGN ADKMLAREET MESEQLKGEL HKVLPVINAS GSDSAMLDNT
LEFLVMSGME LPLAVMITIP EPWANNRYIS EKKKDFYQYY ATMMEPWDGP ASILFSDGDL
VGAVLDRNGL RPSRYYITDE DELILSSEVG VMPISEEHVI KKDRLRPGKM LLVDTVKGEL
VDDDVLKETY ASRRPYGEWL DSNLVELKDI HIPNHRVEHY SNEERRKLQK AFGYTYEEVK
NSILPMAQNG GEPIAAMGHD TPLPMLSKQP QPLFNYFRQR FAQVTNPPID AIREEVVTST
STYIGEDGNL LHEQPENCRV LRIKNPILTD TDLLKIKNMD VPGFKVGVIP ITYYKNTSLK
KAIDRLFLEA DRAYKDGVNV LILSDRGVDE NHVAIPSLLA VSAMQQHLVR TKKRTSVAMI
LESAEPREVH HFATLLGYGA CAVNPYLALD TIKRLIDCNM LDKDYYAAVD DYNSAILHGI
VKIASKMGIS TIQSYQGSKI FEAIGISKEV TDVYFTDTVS RIGGISLQDI ETQVENLHTR
AFDPLDLGND TTLDSLGMHK LRSGKEDHLY NPKTIHLLQQ ATWQKDYKLF KEYSREIDNE
DRFMNLRSLL DFKFPKKGIP LSQVEPVESI VKRFKTGAMS YGSISQEAHE TLAIAMNRLH
GKSNSGEGGE SLERLSIGKD GLNRCSAIKQ VASGRFGVTS HYLVSAKEIQ IKMAQGAKPG
EGGHLPGGKV YPWIAKTRLS TPGVSLISPP PHHDIYSIED LAQLIYDLKC ANTNARISVK
LVSEAGVGTV AAGVAKAGAG VILISGYDGG TGAAPASSIH NAGLPWELGL AETHQTLMMN
GLRSKVVIET DGKLMTGRDV MVAALLGAEE YGFATAPLVT MGCVMMRVCN LDTCPVGVAT
QNPELRKRFK GKPEYVENFM CFIAEEVREL MAKLGVRQLD ELIGRTDLLK VRDDIQDDER
TKKLDLSAII DNPFAKDKKH LYNPKDAYDF KLSKTMDEKV FLKKFKHALE TGEKTKISAR
ISNVDRALGT ILGSEITKNL GDDVPADTFT VECIGSGGQS FGAFIPKGLT LTLEGDSNDY
FGKGLSGGKL VVFPPENVQY KAEDNIIIGN VALYGATSGE AYINGMAGER FCVRNSGAHA
VVEGVGDHGC EYMTGGCVVV LGPTGKNFAA GMSGGIAYVL DENSDLYLKL NKELVSSNAV
TDKYDVMQLK DMIREHVEAT DSERGKEILA NFSEYLPKFK KIIPHDYQAM LQAIAQMEEK
GLSNEQAQIE AFYQLKKAR
//
