ID R7FXD3_9FIRM Unreviewed; 837 AA.
AC R7FXD3;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 48.
DE SubName: Full=DNA polymerase {ECO:0000313|EMBL:CDE19540.1};
GN ORFNames=BN797_00804 {ECO:0000313|EMBL:CDE19540.1};
OS Eubacterium sp. CAG:841.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Eubacteriaceae;
OC Eubacterium.
OX NCBI_TaxID=1262894 {ECO:0000313|EMBL:CDE19540.1, ECO:0000313|Proteomes:UP000017924};
RN [1] {ECO:0000313|EMBL:CDE19540.1, ECO:0000313|Proteomes:UP000017924}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:841 {ECO:0000313|Proteomes:UP000017924};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CDE19540.1}.
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DR EMBL; CBIL010000113; CDE19540.1; -; Genomic_DNA.
DR AlphaFoldDB; R7FXD3; -.
DR STRING; 1262894.BN797_00804; -.
DR Proteomes; UP000017924; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:UniProt.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:InterPro.
DR CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000017924};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 2..260
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
FT DOMAIN 594..801
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
SQ SEQUENCE 837 AA; 93638 MW; AE4AEB99FA3E45FC CRC64;
MKKMLCVDGN SIINRAYYGI RNLTAHDGTP TNAIYGMINI LSRHIAEIEP DYFVVAFDVH
APTFRHKMFS EYKAGRHATP PDLLAQFAPA KECLEAMGAH CVERTGYEAD DILGTYSRIC
AENGIETYIV TGDKDSLQLI DDNTTVLLAT TGESVRYDTA AFRARYGVDP SQFVDMKALM
GDSSDNIPGA AGIGEITAGK LIAEYGSIDA LYDNLEEKKL SAVTKSKLIA SKDMVYLSQK
LARIDRNVQF LPLLERLAYT GPDRKALYAL FRRLDFTLLI KRFGLEDESD DGKTPELPDV
ERADASALKV GVKYGCAIDL YNDEIYLSGD GKLLVIDGIK ENRELLCGGA DLVCYDLKEY
CHSLEKYDVD LSSCKDDVML MAYVCVGGTS DISLGKLVSK HFCTEVVDSA SEAYYVGELY
KVFIDELEKT EQTKLYKNIE LPLARVLYEM ESDGFKVDTA RLVEFSKVLG KAQDELSAKI
YKLADEEFNI NSPRQLGSVL FEKMKLPAGK KTKSGYSTSA EVLESLRPFS PIIDFILEYR
VTGKLKSAYA DGLVAAADKN GRVHTRFRQA LTATGRLSST EPNLQNIPIK TELGRQFRKF
FIPTDENRVL IDADYSQIEL RLLCALSGDE NMREAFLSGF DIHSSTAMKI FGVPKDEVTI
EMRKKAKVIN FGIMYGMGEF SLAEDMHTTR KQAAEYMKNY LQSYPKVQEY LSRVVEQAKK
DGYVTTLFGR RRYIPELASH RRNEVAFGER VAMNSPIQGT AADIIKLAMV AVDKRLKEEK
LDARLLLQVH DELIVEASKS CADKAADILK YEMEHAVELS VPLTVEIAQG QNWYEAH
//
