ID R7IBI1_9FIRM Unreviewed; 818 AA.
AC R7IBI1;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE RecName: Full=Beta-mannosidase B {ECO:0000256|ARBA:ARBA00041069};
DE EC=3.2.1.25 {ECO:0000256|ARBA:ARBA00012754};
DE AltName: Full=Mannanase B {ECO:0000256|ARBA:ARBA00041614};
GN ORFNames=BN770_01950 {ECO:0000313|EMBL:CDE49234.1};
OS Faecalibacterium sp. CAG:74.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae;
OC Faecalibacterium.
OX NCBI_TaxID=1262897 {ECO:0000313|EMBL:CDE49234.1, ECO:0000313|Proteomes:UP000018328};
RN [1] {ECO:0000313|EMBL:CDE49234.1, ECO:0000313|Proteomes:UP000018328}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:74 {ECO:0000313|Proteomes:UP000018328};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing beta-D-mannose residues
CC in beta-D-mannosides.; EC=3.2.1.25;
CC Evidence={ECO:0000256|ARBA:ARBA00000829};
CC -!- PATHWAY: Glycan metabolism; N-glycan degradation.
CC {ECO:0000256|ARBA:ARBA00004740}.
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family. Beta-
CC mannosidase B subfamily. {ECO:0000256|ARBA:ARBA00038429}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CDE49234.1}.
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DR EMBL; CBJB010000139; CDE49234.1; -; Genomic_DNA.
DR AlphaFoldDB; R7IBI1; -.
DR Proteomes; UP000018328; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004567; F:beta-mannosidase activity; IEA:UniProt.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR InterPro; IPR041625; Beta-mannosidase_Ig.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR006102; Glyco_hydro_2_Ig-like.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR041447; Mannosidase_ig.
DR PANTHER; PTHR43730; BETA-MANNOSIDASE; 1.
DR PANTHER; PTHR43730:SF1; BETA-MANNOSIDASE; 1.
DR Pfam; PF00703; Glyco_hydro_2; 1.
DR Pfam; PF17753; Ig_mannosidase; 1.
DR Pfam; PF17786; Mannosidase_ig; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 2.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023001};
KW Cellulose degradation {ECO:0000256|ARBA:ARBA00023001};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023001};
KW Reference proteome {ECO:0000313|Proteomes:UP000018328};
KW Secreted {ECO:0000256|ARBA:ARBA00022525}.
FT DOMAIN 187..274
FT /note="Glycoside hydrolase family 2 immunoglobulin-like
FT beta-sandwich"
FT /evidence="ECO:0000259|Pfam:PF00703"
FT DOMAIN 661..745
FT /note="Mannosidase Ig/CBM-like"
FT /evidence="ECO:0000259|Pfam:PF17786"
FT DOMAIN 749..815
FT /note="Beta-mannosidase Ig-fold"
FT /evidence="ECO:0000259|Pfam:PF17753"
SQ SEQUENCE 818 AA; 93765 MW; 2C6F9F64D9E0AF07 CRC64;
MTEQVNLGGA WRMREADSET WHSAHVPGSV YADLTADGTM PDPFWRENEL DAFERMKKDY
VYQRAFTVTE AQLAHAHVEL VCEGLDTLAH VSLNGREIAF ADNMHITWVW DVKEQLHAGE
NTLEIRFDSP ILYCAKKAEE APGWESSDAT PGFRHLRKAH CMFGWDWGPR LPDAGIWRPI
FLRTWDTARL ENALMLQAHH DGVVDVTIRP EIAGESAWSA EITAPDGEVL TLPETMAAEQ
VIAIQNPQLW WPNGLGKQPL YRVTVRLATG DTRMWRIGLR TMTVSREKDE WGEEFCHVVN
GMKVFAMGAD YIPEDNILAR VTPERTRRLL EDCKAANFNA IRVWGGGYYP DDAFYDICDE
LGLLVWQDLM YACAFYDLTP DFERSIRVET HQNVARLRHH ASLALICGNN EMEMFMAGAN
SALINHRTWE FVPTYPHHIT DYVKMFEYIL PAIVKETAPQ TYWWPASPSS GGNFDAPNDE
NRGDNHYWDV WHGEKPFTEY RKFFFRYASE FGFQSFPCLK SVKQFTLPDD RNIFSRVMER
HQRNQAANGK ILSYLSQTFR YPNSFDDLLY ASQLMQAEAI RYGVEHWRRN RGRCMGAIIW
QLNDIWPVAS WASIDYYGRW KALHYAAKRF FAPVMISAEE EGELSQNPKI NEYHPAPLEK
SFRLNVCNET LRDVTGEVVW ALRTPDGAIV RQNQQTLTIP AMSAKWLDKV DCADASLTGH
YVSFAFVVDD VALSEGTCIF CAPKHFEFVD PRLTLETRGD TLVVTSHAYA KQVWLESEDA
DLLLDDNAFD MNPGTKVVRV VQGSAEKVRG RSVWDLGR
//
