ID R7LN52_9CLOT Unreviewed; 130 AA.
AC R7LN52;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 24-JAN-2024, entry version 35.
DE RecName: Full=Mini-ribonuclease 3 {ECO:0000256|HAMAP-Rule:MF_01468};
DE Short=Mini-3 {ECO:0000256|HAMAP-Rule:MF_01468};
DE Short=Mini-RNase 3 {ECO:0000256|HAMAP-Rule:MF_01468};
DE EC=3.1.26.- {ECO:0000256|HAMAP-Rule:MF_01468};
DE AltName: Full=Mini-RNase III {ECO:0000256|HAMAP-Rule:MF_01468};
DE Short=Mini-III {ECO:0000256|HAMAP-Rule:MF_01468};
GN Name=mrnC {ECO:0000256|HAMAP-Rule:MF_01468};
GN ORFNames=BN638_01059 {ECO:0000313|EMBL:CDE90020.1};
OS Clostridium sp. CAG:389.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=1262801 {ECO:0000313|EMBL:CDE90020.1, ECO:0000313|Proteomes:UP000017978};
RN [1] {ECO:0000313|EMBL:CDE90020.1, ECO:0000313|Proteomes:UP000017978}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:389 {ECO:0000313|Proteomes:UP000017978};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in correct processing of both the 5' and 3' ends of
CC 23S rRNA precursor. Processes 30S rRNA precursor transcript even in
CC absence of ribonuclease 3 (Rnc); Rnc processes 30S rRNA into smaller
CC rRNA precursors. {ECO:0000256|HAMAP-Rule:MF_01468}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01468};
CC -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_01468}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01468}.
CC -!- SIMILARITY: Belongs to the MrnC RNase family. {ECO:0000256|HAMAP-
CC Rule:MF_01468}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CDE90020.1}.
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DR EMBL; CBJV010000009; CDE90020.1; -; Genomic_DNA.
DR AlphaFoldDB; R7LN52; -.
DR STRING; 1262801.BN638_01059; -.
DR Proteomes; UP000017978; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004525; F:ribonuclease III activity; IEA:InterPro.
DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-UniRule.
DR CDD; cd00593; RIBOc; 1.
DR Gene3D; 1.10.1520.10; Ribonuclease III domain; 1.
DR HAMAP; MF_01468; RNase_Mini_III; 1.
DR InterPro; IPR008226; Mini3_fam.
DR InterPro; IPR000999; RNase_III_dom.
DR InterPro; IPR036389; RNase_III_sf.
DR PANTHER; PTHR34276; MINI-RIBONUCLEASE 3; 1.
DR PANTHER; PTHR34276:SF1; MINI-RIBONUCLEASE 3; 1.
DR Pfam; PF00636; Ribonuclease_3; 1.
DR PIRSF; PIRSF005520; UCP005520; 1.
DR SUPFAM; SSF69065; RNase III domain-like; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01468};
KW Endonuclease {ECO:0000256|HAMAP-Rule:MF_01468};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_01468};
KW Magnesium {ECO:0000256|HAMAP-Rule:MF_01468};
KW Nuclease {ECO:0000256|HAMAP-Rule:MF_01468};
KW Reference proteome {ECO:0000313|Proteomes:UP000017978};
KW Ribosome biogenesis {ECO:0000256|HAMAP-Rule:MF_01468};
KW RNA-binding {ECO:0000256|HAMAP-Rule:MF_01468};
KW rRNA processing {ECO:0000256|HAMAP-Rule:MF_01468};
KW rRNA-binding {ECO:0000256|HAMAP-Rule:MF_01468}.
FT DOMAIN 22..118
FT /note="RNase III"
FT /evidence="ECO:0000259|Pfam:PF00636"
FT COILED 56..83
FT /evidence="ECO:0000256|SAM:Coils"
FT ACT_SITE 27
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01468"
SQ SEQUENCE 130 AA; 15366 MW; 1B8F54AD0FD80C79 CRC64;
MNDFIELKKD EQEVNMMPPL TWAYVGDCVY ELYIRTKLVN ETKLKPHALH IEAIKHVKAQ
AQAETLKKIY DELTEKEQEV VRRGRNAENH HLPKNANVQD YMYSTAFEAL IGYLYLTKQN
DRLKEILEKE
//
