ID   R7N5Q2_9FIRM            Unreviewed;       704 AA.
AC   R7N5Q2;
DT   24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT   24-JUL-2013, sequence version 1.
DT   27-MAR-2024, entry version 33.
DE   RecName: Full=Glycosyl hydrolase family 13 catalytic domain-containing protein {ECO:0000259|SMART:SM00642};
GN   ORFNames=BN816_00743 {ECO:0000313|EMBL:CDF08764.1};
OS   Firmicutes bacterium CAG:95.
OC   Bacteria; Bacillota.
OX   NCBI_TaxID=1262988 {ECO:0000313|EMBL:CDF08764.1, ECO:0000313|Proteomes:UP000018182};
RN   [1] {ECO:0000313|EMBL:CDF08764.1, ECO:0000313|Proteomes:UP000018182}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MGS:95 {ECO:0000313|Proteomes:UP000018182};
RA   Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA   Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA   Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA   Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA   Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA   Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA   Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA   Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA   Wang J., Brunak S., Ehrlich S.D.;
RT   "Dependencies among metagenomic species, viruses, plasmids and units of
RT   genetic variation.";
RL   Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:CDF08764.1}.
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DR   EMBL; CBKF010000249; CDF08764.1; -; Genomic_DNA.
DR   AlphaFoldDB; R7N5Q2; -.
DR   STRING; 1262988.BN816_00743; -.
DR   Proteomes; UP000018182; Unassembled WGS sequence.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd11326; AmyAc_Glg_debranch; 1.
DR   CDD; cd11234; E_set_GDE_N; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR004193; Glyco_hydro_13_N.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR   PANTHER; PTHR43002:SF1; ISOAMYLASE 1, CHLOROPLASTIC; 1.
DR   Pfam; PF00128; Alpha-amylase; 2.
DR   Pfam; PF02922; CBM_48; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF81296; E set domains; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   4: Predicted;
KW   Reference proteome {ECO:0000313|Proteomes:UP000018182}.
FT   DOMAIN          170..580
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
FT   REGION          481..503
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        482..503
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   704 AA;  80331 MW;  20B7DE9CECCC25AA CRC64;
     MDGKSMVVTE EKHLIPLDKV NGYDVRPGFY GINGATALRE GVNFTVCSLE ATAVELLLYH
     RGEKEPYAVL PFPKHYRIGG VYSMIVFGLD IYDFEYAYRV DGPYAPEKGL IYDKTQPLLD
     PYARAVTGQS VWGEQPNQDH GYRARVVVND FDWGDHVDPV RPIQDLIIYE LHVRGFTKDA
     SSGVEHPGTF AGIREKIPYL KDLGITAVEL MPVFEFDETM GRREVNGRML LDYWGYNPVS
     FFAPNTSYTA QKEFNREGDE LKGLIRELNA NGIEVILDVV FNHTAEGNEK GPFFSFKGFD
     NRVYYMLTPE GLYYNFSGCG NTLNCNHPVV QQMILECLRY WTIEYHVDGF RFDLASILGR
     NEDGSPMNQP PLLKNLAGDP VLRNVKLIAE AWDAGGLYQV GSFPAFTRWA EWNGKYRDDM
     RSFLKGDYWF AAAAANRLTG SMDLYTGQYK GYDSSINFIT CHDGFSLWDL YSYNGKHNED
     NGWNNSDGHD DNRSWNCGEE GETSDPEIQK LRLRMMKNAC LALMCSRGTP MFLAGDEFGD
     TRFGNNNPYC QDNGISWLDW NRLKTNRELY RFFKKMIRFR KEHPAIRSDQ APSETGFPTV
     SVHTDKPFEH EINSDTKALA VCYAGKTGQG EDLVYVALNV YWEEQRFELP KLPEAYEWRC
     FADTSLDGTG EENGNVPITE YWLQPRTTAV FVGIRKIRAE CQGI
//