ID   R7N7C9_9FIRM            Unreviewed;       425 AA.
AC   R7N7C9;
DT   24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT   24-JUL-2013, sequence version 1.
DT   24-JAN-2024, entry version 27.
DE   SubName: Full=Pyruvate formate lyase {ECO:0000313|EMBL:CDF08556.1};
GN   ORFNames=BN816_00535 {ECO:0000313|EMBL:CDF08556.1};
OS   Firmicutes bacterium CAG:95.
OC   Bacteria; Bacillota.
OX   NCBI_TaxID=1262988 {ECO:0000313|EMBL:CDF08556.1, ECO:0000313|Proteomes:UP000018182};
RN   [1] {ECO:0000313|EMBL:CDF08556.1, ECO:0000313|Proteomes:UP000018182}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MGS:95 {ECO:0000313|Proteomes:UP000018182};
RA   Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA   Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA   Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA   Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA   Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA   Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA   Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA   Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA   Wang J., Brunak S., Ehrlich S.D.;
RT   "Dependencies among metagenomic species, viruses, plasmids and units of
RT   genetic variation.";
RL   Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:CDF08556.1}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CBKF010000227; CDF08556.1; -; Genomic_DNA.
DR   AlphaFoldDB; R7N7C9; -.
DR   STRING; 1262988.BN816_00535; -.
DR   Proteomes; UP000018182; Unassembled WGS sequence.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.20.70.20; -; 1.
DR   InterPro; IPR001150; Gly_radical.
DR   InterPro; IPR004184; PFL_dom.
DR   PANTHER; PTHR43641:SF2; DEHYDRATASE YBIW-RELATED; 1.
DR   PANTHER; PTHR43641; FORMATE ACETYLTRANSFERASE 3-RELATED; 1.
DR   Pfam; PF01228; Gly_radical; 1.
DR   Pfam; PF02901; PFL-like; 1.
DR   SUPFAM; SSF51998; PFL-like glycyl radical enzymes; 1.
DR   PROSITE; PS51149; GLY_RADICAL_2; 1.
DR   PROSITE; PS51554; PFL; 1.
PE   4: Predicted;
KW   Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000313|EMBL:CDF08556.1};
KW   Organic radical {ECO:0000256|ARBA:ARBA00022818, ECO:0000256|PROSITE-
KW   ProRule:PRU00493}; Pyruvate {ECO:0000313|EMBL:CDF08556.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000018182}.
FT   DOMAIN          1..301
FT                   /note="PFL"
FT                   /evidence="ECO:0000259|PROSITE:PS51554"
FT   DOMAIN          308..425
FT                   /note="Glycine radical"
FT                   /evidence="ECO:0000259|PROSITE:PS51149"
FT   REGION          293..312
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         402
FT                   /note="Glycine radical"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00493"
SQ   SEQUENCE   425 AA;  48066 MW;  BE4863A40E9867E7 CRC64;
     MILEIFEEQE LVNPKLQARI SHNHSMEYFE KLGKIIAKGK NVLSIFNDEI LIPANVRQGK
     ELVDARLYVA GGCQEPLLSE TEINCRAYIY VSLPKLLLLI LGVGTQDFWI HEGIEQPNVH
     TCLNYAEFYS CFVGKIRQII YQVAKYFNQY EKMWYEYNPC PLYSATITGC IEKAKDMTQG
     GAKYNTSSFS PVGFGTLVDS LFAIKKVVFE DKDMTLDELI KCLNSNFSDC ERTRAFLTYK
     VDKWGRDSGE INQLASEVSE DLSQIFSGIP NSRGGWFESS LFSNTGYVSL RNTEATPDGR
     KQGDILSRGM GPSEAVGPTN ISRIMEGLDK LRMENYPASA VLYLDFPFSP NNMDSTIFSA
     VVRSFLDMGG NVVDFNITDS GVLEEAKKNP EKYKNLIVRV WGFSAYFTAL DEELQNEMIN
     RNRAH
//