ID R7XZB8_9ACTN Unreviewed; 683 AA.
AC R7XZB8;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 53.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=CF8_1250 {ECO:0000313|EMBL:EON24679.1};
OS Nocardioides sp. CF8.
OC Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
OC Nocardioidaceae; Nocardioides.
OX NCBI_TaxID=110319 {ECO:0000313|EMBL:EON24679.1, ECO:0000313|Proteomes:UP000015971};
RN [1] {ECO:0000313|EMBL:EON24679.1, ECO:0000313|Proteomes:UP000015971}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CF8 {ECO:0000313|EMBL:EON24679.1,
RC ECO:0000313|Proteomes:UP000015971};
RX PubMed=23833136;
RA Kimbrel J.A., Chang J., Arp D.J., Sayavedra-Soto L.A.;
RT "The Draft Genome Sequence of Nocardioides sp. Strain CF8 Reveals the Scope
RT of Its Metabolic Capabilities.";
RL Genome Announc. 1:e00439-e00439(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EON24679.1}.
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DR EMBL; ASEP01000042; EON24679.1; -; Genomic_DNA.
DR RefSeq; WP_010832234.1; NZ_CM001852.1.
DR AlphaFoldDB; R7XZB8; -.
DR STRING; 110319.CF8_1250; -.
DR PATRIC; fig|110319.3.peg.1846; -.
DR eggNOG; COG0210; Bacteria.
DR HOGENOM; CLU_004585_5_6_11; -.
DR OrthoDB; 9806690at2; -.
DR Proteomes; UP000015971; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 1.10.150.80; HRDC domain; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR010997; HRDC-like_sf.
DR InterPro; IPR002121; HRDC_dom.
DR InterPro; IPR044876; HRDC_dom_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF45; ATP-DEPENDENT DNA HELICASE UVRD2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00570; HRDC; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 2.
DR SMART; SM00341; HRDC; 1.
DR SUPFAM; SSF47819; HRDC-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS50967; HRDC; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000015971}.
FT DOMAIN 9..290
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 291..536
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT DOMAIN 608..683
FT /note="HRDC"
FT /evidence="ECO:0000259|PROSITE:PS50967"
FT BINDING 30..37
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 683 AA; 74079 MW; 1940C51A7DC4BBA0 CRC64;
MPTPDELLAA LDPEQRQVAE AIRGPVRVLA GAGTGKTRAI THRIAYGVAT GVYAPTEVLA
VTFTTRAAGE MRGRLRTLGA GGVQARTFHS AALRQLRYFW PRVHGHELPE LIDSKIGLLA
GAARRQRVQT DQATLRDLAS EVEWAKVSNV SPDDYARVAD QRGRSVSHLD ADSVARIYQG
YEEIKRGQTR MDMEDVLLFA AGLLADEEAV AAQVRRQYKW LVVDEFQDVS PLQSALLDLW
LGGRDEICVV GDPAQTIYSF AGANASYLRD FGKKFPAATS VELVRNYRST PQIVGAANQM
LAGTPSKGVE LLAQQPAGPE VTYRAHPDEV AEAAAAADKI ATLRSAGIPA GEIAILFRIN
AQSETFEDAL TQRKIPYVVR GAARFFDRAE VRQAVTLLRG TARSGQAEGS VVELVQATLS
GMGWNTEPPS TRGETRNRWE SLQALVDQAA EFVREHPGAE LGDLVDDLDR RAAEQHAPLA
DGVTLATLHT AKGLEWDAVF LCGMQDGTLP ITYADTPAAI EEERRLLYVG VTRARKHLAI
SWSLARNPGG RGSRKPSRFL NGVAEVERSA AVSTSVSRNR KARHCRECGG ALASPSEKKI
GRCSDCPASY DEALFERLRE WRLARAADDS VPAFVVFTDA TLQLIAEHCP SDERGLLKIS
GVGASKLAKY GDDVLAVLAG KEI
//
