ID   R8ASK4_PLESH            Unreviewed;       926 AA.
AC   R8ASK4;
DT   24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT   24-JUL-2013, sequence version 1.
DT   27-MAR-2024, entry version 33.
DE   SubName: Full=Putative zinc-dependent peptidase {ECO:0000313|EMBL:EON89306.1};
GN   ORFNames=PLESHI_06172 {ECO:0000313|EMBL:EON89306.1};
OS   Plesiomonas shigelloides 302-73.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Plesiomonas.
OX   NCBI_TaxID=1315976 {ECO:0000313|EMBL:EON89306.1, ECO:0000313|Proteomes:UP000014012};
RN   [1] {ECO:0000313|EMBL:EON89306.1, ECO:0000313|Proteomes:UP000014012}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=302-73 {ECO:0000313|EMBL:EON89306.1,
RC   ECO:0000313|Proteomes:UP000014012};
RX   PubMed=23814109;
RA   Pique N., Aquilini E., Alioto T., Minana-Galbis D., Tomas J.M.;
RT   "Genome Sequence of Plesiomonas shigelloides Strain 302-73 (Serotype O1).";
RL   Genome Announc. 1:e00404-13(2013).
CC   -!- SIMILARITY: Belongs to the peptidase M16 family.
CC       {ECO:0000256|ARBA:ARBA00007261, ECO:0000256|RuleBase:RU004447}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:EON89306.1}.
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DR   EMBL; AQQO01000038; EON89306.1; -; Genomic_DNA.
DR   RefSeq; WP_010862859.1; NZ_KB944507.1.
DR   AlphaFoldDB; R8ASK4; -.
DR   STRING; 703.SAMEA2665130_00140; -.
DR   PATRIC; fig|1315976.3.peg.1215; -.
DR   HOGENOM; CLU_008156_2_0_6; -.
DR   Proteomes; UP000014012; Unassembled WGS sequence.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.830.10; Metalloenzyme, LuxS/M16 peptidase-like; 4.
DR   InterPro; IPR011249; Metalloenz_LuxS/M16.
DR   InterPro; IPR011765; Pept_M16_N.
DR   InterPro; IPR001431; Pept_M16_Zn_BS.
DR   InterPro; IPR007863; Peptidase_M16_C.
DR   PANTHER; PTHR43690:SF18; INSULIN-DEGRADING ENZYME-RELATED; 1.
DR   PANTHER; PTHR43690; NARDILYSIN; 1.
DR   Pfam; PF00675; Peptidase_M16; 1.
DR   Pfam; PF05193; Peptidase_M16_C; 2.
DR   SUPFAM; SSF63411; LuxS/MPP-like metallohydrolase; 3.
DR   PROSITE; PS00143; INSULINASE; 1.
PE   3: Inferred from homology;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Metalloprotease {ECO:0000256|ARBA:ARBA00023049};
KW   Protease {ECO:0000256|ARBA:ARBA00022670};
KW   Reference proteome {ECO:0000313|Proteomes:UP000014012};
KW   Signal {ECO:0000256|SAM:SignalP}; Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT   SIGNAL          1..17
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           18..926
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5004451437"
FT   DOMAIN          45..167
FT                   /note="Peptidase M16 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00675"
FT   DOMAIN          199..371
FT                   /note="Peptidase M16 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF05193"
FT   DOMAIN          674..852
FT                   /note="Peptidase M16 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF05193"
SQ   SEQUENCE   926 AA;  103533 MW;  712EFAF12E7C37AF CRC64;
     MTWLAAALML VATQVAAEAL QPDPAWLEGR LDNGLHWQIL NTPQRPNDRI ELRLLVRTGS
     LQERPEQAGY AHFLEHMAFN GTARFPEQAI RDLWQQAGLT FGDDLNASTE YDNTLYRISL
     PADKPQLLTP ALTWLSDVAG GLSLQPAQVS AERGVILGES LTRDKNQRSA HQQWWDYQIK
     GSALAKHDPI GEAEVINSAT RERLQAFYRQ WYTPDAMTLL ISGNVDARKV SSEVAHLFAP
     LQGKRSEAVP PAVLPPLAAS PLQISAHKQL NNQLLLVWDS NWQPPQDSAA LDALWLGDFV
     RGLIRQRLSD AISQHGSQPI SLTLGCSVRY ARESCALSVE SDAEQRQKWL QFVATELARL
     RQHGVLPSEL TLAHRLANDD LNRFYEQYAR MTSGDFINQR LFALESGAPD LGPEQYRRLR
     ATFLQNLSLE RVNQELQRQL AIEPVLVLMQ PHSEAAQSAD SLMQAWRFLS AQPQAPLSRS
     ESGAAAAQQM LATPAQIGQI TEQQSWPQWK LSRALLSNGL SVLVRQDPQA KDRIYLRLRS
     RGGVRSLPVE LLPASQLLGV WGAHGVGPLD RTQTQQFMDV NRLDMWPLLT LSQHGLGGSS
     DVEHLDAWLA MARQSLLAPK LDNQVLDDVR TQQASQLTQY LATAQGRFNQ ALDQHLFPHN
     RYAELPTPAQ LKQVSLKQLQ ALHQIFISTM QDAELVVVGD VDPAKVFALA ARYLGGIDLG
     GEPEWARYPF LLRQSKPLLR QDNPEDRTLV IESRLSVPAM QDDLAHRLAH DMLLRILQQR
     LENQLREALG LTYGVAVDMD YPSTDMQLNL LQIRVLVAPD KQEEAQETIA EQLALLLDKG
     ISEQEFTQAR GQVATSLQDI WRQPAATVEL LSQYRLAGYP LEEVMDVNQV LNGITEQTVR
     DLMLHYLRAP GQLSAVFAPP VAAKSK
//