ID R9JKL6_9FIRM Unreviewed; 386 AA.
AC R9JKL6;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 41.
DE RecName: Full=site-specific DNA-methyltransferase (cytosine-N(4)-specific) {ECO:0000256|ARBA:ARBA00012185};
DE EC=2.1.1.113 {ECO:0000256|ARBA:ARBA00012185};
GN ORFNames=C804_01501 {ECO:0000313|EMBL:EOS34668.1};
OS Lachnospiraceae bacterium A4.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae.
OX NCBI_TaxID=397291 {ECO:0000313|EMBL:EOS34668.1, ECO:0000313|Proteomes:UP000014118};
RN [1] {ECO:0000313|EMBL:EOS34668.1, ECO:0000313|Proteomes:UP000014118}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=A4 {ECO:0000313|EMBL:EOS34668.1,
RC ECO:0000313|Proteomes:UP000014118};
RG The Broad Institute Genomics Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Earl A., Xavier R., Elson C., Duck W., Walker B., Young S., Zeng Q.,
RA Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Allen A.W., Alvarado L.,
RA Arachchi H.M., Berlin A.M., Chapman S.B., Gainer-Dewar J., Goldberg J.,
RA Griggs A., Gujja S., Hansen M., Howarth C., Imamovic A., Ireland A.,
RA Larimer J., McCowan C., Murphy C., Pearson M., Poon T.W., Priest M.,
RA Roberts A., Saif S., Shea T., Sisk P., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The Genome Sequence of Lachnospiraceae bacterium A4.";
RL Submitted (APR-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = an N(4)-
CC methyl-2'-deoxycytidine in DNA + H(+) + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:16857, Rhea:RHEA-COMP:11369, Rhea:RHEA-COMP:13674,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:85452, ChEBI:CHEBI:137933; EC=2.1.1.113;
CC Evidence={ECO:0000256|ARBA:ARBA00001893};
CC -!- SIMILARITY: Belongs to the N(4)/N(6)-methyltransferase family. N(4)
CC subfamily. {ECO:0000256|ARBA:ARBA00010203}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EOS34668.1}.
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DR EMBL; ASSR01000028; EOS34668.1; -; Genomic_DNA.
DR AlphaFoldDB; R9JKL6; -.
DR STRING; 397291.C804_01501; -.
DR PATRIC; fig|397291.3.peg.1531; -.
DR eggNOG; COG0863; Bacteria.
DR HOGENOM; CLU_060286_1_0_9; -.
DR OrthoDB; 9773571at2; -.
DR Proteomes; UP000014118; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0015667; F:site-specific DNA-methyltransferase (cytosine-N4-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:InterPro.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 1.10.1660.10; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 2.
DR InterPro; IPR009061; DNA-bd_dom_put_sf.
DR InterPro; IPR002941; DNA_methylase_N4/N6.
DR InterPro; IPR041657; HTH_17.
DR InterPro; IPR000551; MerR-type_HTH_dom.
DR InterPro; IPR017985; MeTrfase_CN4_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR010093; SinI_DNA-bd.
DR NCBIfam; TIGR01764; excise; 1.
DR Pfam; PF12728; HTH_17; 1.
DR Pfam; PF01555; N6_N4_Mtase; 1.
DR SUPFAM; SSF46955; Putative DNA-binding domain; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS50937; HTH_MERR_2; 1.
DR PROSITE; PS00093; N4_MTASE; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000014118};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 6..50
FT /note="HTH merR-type"
FT /evidence="ECO:0000259|PROSITE:PS50937"
FT REGION 84..105
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 87..105
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 386 AA; 44537 MW; 23F5A1FF91EA13E6 CRC64;
MNENSSITTQ RAAEMLGVSV SSIYRMEKND ILHPIRTPGG QRRFKLEELE QYKINSVNIV
APQKPYQEAG GRGSFASKGI NQREFTTEQQ GEKEADEESA VRKEVDRRNT LNDLNGTEWL
PETKSFFYQK GLGSKHPHAQ IERQHPAPYS FQDIVHLITF FTKKGMHVLD PFGGVGSTAK
ACALEKRVCT SIELSEKWHD LAIQRLNYEV AEGEAENHHM INGDTRDVLK LFEDNMFDFV
VTSPPYWSIL NKKADYKVKK ERLSNNLATN YSDNDDNDLA NIEDYKTFLD VLSDDVLMQC
ARVLKPKKYM CLVVSDFRHK SEYISFHSDL IQRLNKRQIQ GKMGLHLQGT KILLQNHKSL
LPYGYPFAYV ENIHHQYVLI FRKDEI
//
