ID R9KRJ2_9FIRM Unreviewed; 738 AA.
AC R9KRJ2;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE RecName: Full=Alpha-galactosidase {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
DE EC=3.2.1.22 {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
GN ORFNames=C810_02245 {ECO:0000313|EMBL:EOS46147.1};
OS Lachnospiraceae bacterium A2.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae.
OX NCBI_TaxID=397290 {ECO:0000313|EMBL:EOS46147.1, ECO:0000313|Proteomes:UP000014150};
RN [1] {ECO:0000313|EMBL:EOS46147.1, ECO:0000313|Proteomes:UP000014150}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=A2 {ECO:0000313|EMBL:EOS46147.1,
RC ECO:0000313|Proteomes:UP000014150};
RG The Broad Institute Genomics Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Earl A., Xavier R., Elson C., Duck W., Walker B., Young S., Zeng Q.,
RA Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Allen A.W., Alvarado L.,
RA Arachchi H.M., Berlin A.M., Chapman S.B., Gainer-Dewar J., Goldberg J.,
RA Griggs A., Gujja S., Hansen M., Howarth C., Imamovic A., Ireland A.,
RA Larimer J., McCowan C., Murphy C., Pearson M., Poon T.W., Priest M.,
RA Roberts A., Saif S., Shea T., Sisk P., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The Genome Sequence of Lachnospiraceae bacterium A2.";
RL Submitted (APR-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing alpha-D-galactose
CC residues in alpha-D-galactosides, including galactose
CC oligosaccharides, galactomannans and galactolipids.; EC=3.2.1.22;
CC Evidence={ECO:0000256|ARBA:ARBA00001255,
CC ECO:0000256|PIRNR:PIRNR005536};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase.
CC {ECO:0000256|PIRNR:PIRNR005536}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EOS46147.1}.
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DR EMBL; ASSX01000009; EOS46147.1; -; Genomic_DNA.
DR AlphaFoldDB; R9KRJ2; -.
DR STRING; 397290.C810_02245; -.
DR PATRIC; fig|397290.3.peg.2486; -.
DR eggNOG; COG3345; Bacteria.
DR HOGENOM; CLU_009640_2_1_9; -.
DR OrthoDB; 9758822at2; -.
DR Proteomes; UP000014150; Unassembled WGS sequence.
DR GO; GO:0004557; F:alpha-galactosidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016052; P:carbohydrate catabolic process; IEA:InterPro.
DR CDD; cd14791; GH36; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR Gene3D; 2.70.98.60; alpha-galactosidase from lactobacil brevis; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR038417; Alpga-gal_N_sf.
DR InterPro; IPR002252; Glyco_hydro_36.
DR InterPro; IPR031705; Glyco_hydro_36_C.
DR InterPro; IPR031704; Glyco_hydro_36_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR43053:SF3; ALPHA-GALACTOSIDASE C-RELATED; 1.
DR PANTHER; PTHR43053; GLYCOSIDASE FAMILY 31; 1.
DR Pfam; PF16874; Glyco_hydro_36C; 1.
DR Pfam; PF16875; Glyco_hydro_36N; 1.
DR Pfam; PF02065; Melibiase; 1.
DR PIRSF; PIRSF005536; Agal; 1.
DR PRINTS; PR00743; GLHYDRLASE36.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PIRNR:PIRNR005536};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR005536};
KW Reference proteome {ECO:0000313|Proteomes:UP000014150}.
FT DOMAIN 27..285
FT /note="Glycosyl hydrolase family 36 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF16875"
FT DOMAIN 650..728
FT /note="Glycosyl hydrolase family 36 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF16874"
FT ACT_SITE 478
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
FT ACT_SITE 548
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
SQ SEQUENCE 738 AA; 84866 MW; CB943D352E2B31E1 CRC64;
MAIIIKDNVF TLQTKNSTYQ MKADSKGVLL HTYYGKKTDE TDYSYLISMQ DRGFSGNIYE
AREDRTYSLD FLPQEFPVRG SGDYRISCMH ADLGTGVRDC LLFFDSYRTY HGKYGLEGLP
AMFASEDEPV DTLEITLKDS QEEFYLHLYY GVFEEHDIIT RAAVVENRTD QEIRLSKVLS
ACLDMDHDGL DFIHFYGRHT RERKMERVPL FHGVTELRST RGTSSHQHNP FVILADKTTS
EEMGECYGFS LLYSGGFHLH AEVDQFHQTR LVVGIDDSVF TWSLQPGERF VAPEVAMAYS
NGGLSQLSHC YHNGFQKNLI RSRWKDKRRP ILVNNWEATY FDFNAENLLK IAEEAKELEL
DMLVLDDGWF GKRNDDFSGL GDWYVNEEKL GCTLKELVDK VNGMGLQFGL WFEPEMVSED
SDLYRNHPDW AMVVPDRRPI RSRSQLVLDM SREDVRDYIK ERLFAILDSA NIQYVKWDMN
RSVAAAYSVL LPRERQGELR HRYVLGLYEV MEAMLERYPD LLLEGCSGGG GRFDAGMLYY
APQIWCSDNT DAIERLVIHH GTSFGYPMSS VSAHVSVCPN HQNGRVTPFR TRAICAMQGT
FGYELDLSKL SKEEKAEVKQ QVRMFKERYR LFQFGNYYRI SSPLENRDFT AWEYADKDGK
EAYIGVVYTD LHGNPASWCI KFQGLLPEGK YTMWKNNEPA GTFTGVALMS GGVLLPVPVE
NYDSCQIYVR LEGESGRG
//
