ID R9M4K8_9FIRM Unreviewed; 664 AA.
AC R9M4K8;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 41.
DE RecName: Full=Cyclic-di-AMP phosphodiesterase {ECO:0000256|PIRNR:PIRNR026583};
DE EC=3.1.4.- {ECO:0000256|PIRNR:PIRNR026583};
GN ORFNames=C816_01759 {ECO:0000313|EMBL:EOS65905.1};
OS Oscillibacter sp. 1-3.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae;
OC Oscillibacter.
OX NCBI_TaxID=1235797 {ECO:0000313|EMBL:EOS65905.1, ECO:0000313|Proteomes:UP000014108};
RN [1] {ECO:0000313|EMBL:EOS65905.1, ECO:0000313|Proteomes:UP000014108}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1-3 {ECO:0000313|EMBL:EOS65905.1,
RC ECO:0000313|Proteomes:UP000014108};
RG The Broad Institute Genomics Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Earl A., Xavier R., Elson C., Duck W., Walker B., Young S., Zeng Q.,
RA Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Allen A.W., Alvarado L.,
RA Arachchi H.M., Berlin A.M., Chapman S.B., Gainer-Dewar J., Goldberg J.,
RA Griggs A., Gujja S., Hansen M., Howarth C., Imamovic A., Ireland A.,
RA Larimer J., McCowan C., Murphy C., Pearson M., Poon T.W., Priest M.,
RA Roberts A., Saif S., Shea T., Sisk P., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The Genome Sequence of Oscillibacter bacterium 1-3.";
RL Submitted (APR-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Has phosphodiesterase (PDE) activity against cyclic-di-AMP
CC (c-di-AMP). {ECO:0000256|PIRNR:PIRNR026583}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3',3'-c-di-AMP + H2O = 5'-O-phosphonoadenylyl-(3'->5')-
CC adenosine + H(+); Xref=Rhea:RHEA:54420, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:71500, ChEBI:CHEBI:138171;
CC Evidence={ECO:0000256|PIRNR:PIRNR026583};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004651};
CC Multi-pass membrane protein {ECO:0000256|ARBA:ARBA00004651}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the GdpP/PdeA phosphodiesterase family.
CC {ECO:0000256|PIRNR:PIRNR026583}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EOS65905.1}.
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DR EMBL; ASTC01000013; EOS65905.1; -; Genomic_DNA.
DR RefSeq; WP_016322063.1; NZ_KE159707.1.
DR AlphaFoldDB; R9M4K8; -.
DR STRING; 1235797.C816_01759; -.
DR PATRIC; fig|1235797.3.peg.1917; -.
DR eggNOG; COG3887; Bacteria.
DR HOGENOM; CLU_018278_0_0_9; -.
DR OrthoDB; 9759476at2; -.
DR Proteomes; UP000014108; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0106409; F:cyclic-di-AMP phosphodiesterase activity; IEA:RHEA.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:UniProtKB-UniRule.
DR Gene3D; 3.10.310.30; -; 1.
DR Gene3D; 3.90.1640.10; inorganic pyrophosphatase (n-terminal core); 1.
DR Gene3D; 3.30.450.20; PAS domain; 1.
DR InterPro; IPR001667; DDH_dom.
DR InterPro; IPR038763; DHH_sf.
DR InterPro; IPR003156; DHHA1_dom.
DR InterPro; IPR014528; GdpP/PdeA.
DR InterPro; IPR000160; GGDEF_dom.
DR PANTHER; PTHR47618; BIFUNCTIONAL OLIGORIBONUCLEASE AND PAP PHOSPHATASE NRNA; 1.
DR PANTHER; PTHR47618:SF2; CYCLIC-DI-AMP PHOSPHODIESTERASE GDPP; 1.
DR Pfam; PF01368; DHH; 1.
DR Pfam; PF02272; DHHA1; 1.
DR PIRSF; PIRSF026583; YybT; 1.
DR SMART; SM00267; GGDEF; 1.
DR SUPFAM; SSF64182; DHH phosphoesterases; 1.
DR PROSITE; PS50887; GGDEF; 1.
PE 3: Inferred from homology;
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475,
KW ECO:0000256|PIRNR:PIRNR026583}; Hydrolase {ECO:0000256|PIRNR:PIRNR026583};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|PIRNR:PIRNR026583};
KW Reference proteome {ECO:0000313|Proteomes:UP000014108};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989}.
FT DOMAIN 189..317
FT /note="GGDEF"
FT /evidence="ECO:0000259|PROSITE:PS50887"
SQ SEQUENCE 664 AA; 72995 MW; AD9E6C28D8F1BFE5 CRC64;
MNNKKLSRML EPNLKLYFLC MALFCLLALA VNPLLALAEA CVTAALYAYF SQANKKRRQG
ILQYIDSVTG SVDTASKSTL IHSPLPIMVF RPDTGEVIWS NEDFLQLAGV REHLFEMRVE
DAVPNCPVQW LLEGKQECPE RVAMNGRRFR VYGSLVRAKS RSGEQNLVAT TYWVDVTESD
ALREAYTATR PVLALLMVDN YEDLMKACAD TQRSGILAQI DEKLDQWAAG SDGILLKTER
DRYLFLFEER HYDHFVEEKF SVLDTIRDIK AAEGVPPTLS IGIGKDGATL SELLKNANLS
LEMALSRGGD QAVVRGKVDF AFYGGRAKAT EKRTKVKSRV MANALSELMA DAGQIYVMGH
SYADMDAVGA AVGICCAARK RGKQAQIVMD LEHNASEALL ERVRSMPEYA DTFISGGEAF
LKMQPGALLV VVDTNRPELV DSPQVLDACN RVAVIDHHRR AATYIENAAF SFHEPYASSA
SELVTELLQY LIEPADLMRG EAEALLAGIV LDTKHFTLRT GGRTFEAAAF LRRAGADTAD
VQRLFQNDLN DMVSRYDIVR RAELYRGDIA VSVVPQEGVD RVAAAQAADE LLTLKGVKAS
FVLYRNGENV LMSARSLGEV NVQVVLEALG GGGNSTTAGG CVENGDILDV RTRLVAAIDA
YFEK
//
