ID   R9MS71_9FIRM            Unreviewed;       792 AA.
AC   R9MS71;
DT   24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT   24-JUL-2013, sequence version 1.
DT   27-MAR-2024, entry version 36.
DE   SubName: Full=PFL2/glycerol dehydratase family glycyl radical enzyme {ECO:0000313|EMBL:EOS70652.1};
GN   ORFNames=C818_01249 {ECO:0000313|EMBL:EOS70652.1};
OS   Lachnospiraceae bacterium MD308.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae.
OX   NCBI_TaxID=1235799 {ECO:0000313|EMBL:EOS70652.1, ECO:0000313|Proteomes:UP000014117};
RN   [1] {ECO:0000313|EMBL:EOS70652.1, ECO:0000313|Proteomes:UP000014117}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=3-2 {ECO:0000313|EMBL:EOS70652.1,
RC   ECO:0000313|Proteomes:UP000014117};
RG   The Broad Institute Genomics Platform;
RG   The Broad Institute Genome Sequencing Center for Infectious Disease;
RA   Earl A., Xavier R., Elson C., Duck W., Walker B., Young S., Zeng Q.,
RA   Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Allen A.W., Alvarado L.,
RA   Arachchi H.M., Berlin A.M., Chapman S.B., Gainer-Dewar J., Goldberg J.,
RA   Griggs A., Gujja S., Hansen M., Howarth C., Imamovic A., Ireland A.,
RA   Larimer J., McCowan C., Murphy C., Pearson M., Poon T.W., Priest M.,
RA   Roberts A., Saif S., Shea T., Sisk P., Sykes S., Wortman J., Nusbaum C.,
RA   Birren B.;
RT   "The Genome Sequence of Lachnospiraceae bacterium 3-2.";
RL   Submitted (APR-2013) to the EMBL/GenBank/DDBJ databases.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:EOS70652.1}.
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DR   EMBL; ASTE01000012; EOS70652.1; -; Genomic_DNA.
DR   AlphaFoldDB; R9MS71; -.
DR   STRING; 1235799.C818_01249; -.
DR   PATRIC; fig|1235799.3.peg.1332; -.
DR   eggNOG; COG1882; Bacteria.
DR   HOGENOM; CLU_009096_0_1_9; -.
DR   OrthoDB; 9803969at2; -.
DR   Proteomes; UP000014117; Unassembled WGS sequence.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   CDD; cd01677; PFL2_DhaB_BssA; 1.
DR   Gene3D; 3.20.70.20; -; 1.
DR   InterPro; IPR001150; Gly_radical.
DR   InterPro; IPR004184; PFL_dom.
DR   PANTHER; PTHR43641:SF2; DEHYDRATASE YBIW-RELATED; 1.
DR   PANTHER; PTHR43641; FORMATE ACETYLTRANSFERASE 3-RELATED; 1.
DR   Pfam; PF01228; Gly_radical; 1.
DR   Pfam; PF02901; PFL-like; 1.
DR   SUPFAM; SSF51998; PFL-like glycyl radical enzymes; 1.
DR   PROSITE; PS51149; GLY_RADICAL_2; 1.
DR   PROSITE; PS51554; PFL; 1.
PE   4: Predicted;
KW   Lyase {ECO:0000256|ARBA:ARBA00023239};
KW   Organic radical {ECO:0000256|ARBA:ARBA00022818, ECO:0000256|PROSITE-
KW   ProRule:PRU00493}; Reference proteome {ECO:0000313|Proteomes:UP000014117}.
FT   DOMAIN          9..665
FT                   /note="PFL"
FT                   /evidence="ECO:0000259|PROSITE:PS51554"
FT   DOMAIN          672..792
FT                   /note="Glycine radical"
FT                   /evidence="ECO:0000259|PROSITE:PS51149"
FT   REGION          664..685
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         767
FT                   /note="Glycine radical"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00493"
SQ   SEQUENCE   792 AA;  89766 MW;  2DBACE8564FBE376 CRC64;
     MAKLRGMNER IQKLRELSVT TPVHIDLERA KIETDFYKAN DGKYSIPVMR AMVLKEYFSR
     KTLYLGDGEL IIGEKGKDPQ ASPTFPELCC HSVEDMVVMS ERELVSFKTT EEDRKLQAEE
     IIPYWTGRSM REKILARMTP EWHECYSAGM FTEFMEQRGP GHTCGGEQVF TTGYLDYKEK
     IQKTMDALDY INDPDAVNKC EELKAMDIAC DAVIILGERY HRLALEMAEK ETDETRKAEL
     LQIAANLEVV PAHSPKTYWQ AIQLYWFTHL AVTTELNPWD AFSPGRLDQH LNPYYERDVE
     AGILDDERAL ELLECLWVKF YNQPAPVKVG ITLKESATYT DFANINTGGV TPDGRNGVNN
     VSYLILDCMD EMKLVQPNSN VTISKKTPAK FLKRACEVSR EGWGQPAFYN TEAQIMELIN
     AGKKLEDARR GGSSGCVETG AWGSEAYILT GYLNIPKVFQ LTLFNGFDQY SGKQIGLELG
     YAKDFKTYDE LWDAFKKQLE HIVNIKIRGN NVIEQLYAQE MPAPCLSVVT NDCISNAKDY
     NAGGARYNTN YIQGVGIGTV TDCLAAVKYN VFDNQNFTME ELIEAMEHDY EGYDAIYRMV
     HDKTPKYGND DDYADDIMQE VFELYRSEIT GRPNMKGGKY GVDMLPTTCH VYFGDVILAT
     PNGRKAHKPV SEGISPEKSA DRNGPTAVIK SCSKMDHLAT AGTLLNQKFT PNVVAGEKGL
     DNMASLIRSY FSMDGHHIQF NVIDRQTLID AQKNPEEYKD LIVRVAGYSD FFRNLDKPLQ
     DEIIERTEQS FE
//