ID R9NA88_9FIRM Unreviewed; 442 AA.
AC R9NA88;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 46.
DE SubName: Full=Radical SAM additional 4Fe4S-binding SPASM domain-containing protein {ECO:0000313|EMBL:EOS80028.1};
GN ORFNames=C817_02137 {ECO:0000313|EMBL:EOS80028.1};
OS Dorea sp. 5-2.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae; Dorea.
OX NCBI_TaxID=1235798 {ECO:0000313|EMBL:EOS80028.1, ECO:0000313|Proteomes:UP000014211};
RN [1] {ECO:0000313|EMBL:EOS80028.1, ECO:0000313|Proteomes:UP000014211}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=5-2 {ECO:0000313|EMBL:EOS80028.1,
RC ECO:0000313|Proteomes:UP000014211};
RG The Broad Institute Genomics Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Earl A., Xavier R., Elson C., Duck W., Walker B., Young S., Zeng Q.,
RA Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Allen A.W., Alvarado L.,
RA Arachchi H.M., Berlin A.M., Chapman S.B., Gainer-Dewar J., Goldberg J.,
RA Griggs A., Gujja S., Hansen M., Howarth C., Imamovic A., Ireland A.,
RA Larimer J., McCowan C., Murphy C., Pearson M., Poon T.W., Priest M.,
RA Roberts A., Saif S., Shea T., Sisk P., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The Genome Sequence of Dorea bacterium 5-2.";
RL Submitted (APR-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SIMILARITY: Belongs to the radical SAM superfamily. Anaerobic
CC sulfatase-maturating enzyme family. {ECO:0000256|ARBA:ARBA00023601}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EOS80028.1}.
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DR EMBL; ASTD01000038; EOS80028.1; -; Genomic_DNA.
DR RefSeq; WP_016218874.1; NZ_KE159759.1.
DR AlphaFoldDB; R9NA88; -.
DR STRING; 1235798.C817_02137; -.
DR PATRIC; fig|1235798.3.peg.2255; -.
DR eggNOG; COG0641; Bacteria.
DR HOGENOM; CLU_009273_3_1_9; -.
DR OrthoDB; 9808591at2; -.
DR UniPathway; UPA00782; -.
DR Proteomes; UP000014211; Unassembled WGS sequence.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR CDD; cd01335; Radical_SAM; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR023885; 4Fe4S-binding_SPASM_dom.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023867; Sulphatase_maturase_rSAM.
DR NCBIfam; TIGR04085; rSAM_more_4Fe4S; 1.
DR PANTHER; PTHR43787:SF3; FEMO COFACTOR BIOSYNTHESIS PROTEIN NIFB; 1.
DR PANTHER; PTHR43787; FEMO COFACTOR BIOSYNTHESIS PROTEIN NIFB-RELATED; 1.
DR Pfam; PF13353; Fer4_12; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01386; main_SPASM_domain-containing; 1.
DR SFLD; SFLDG01384; thioether_bond_formation_requi; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 3: Inferred from homology;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000014211};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT DOMAIN 92..319
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 442 AA; 51077 MW; A2713A0E91F4600E CRC64;
MPFNNTLFKD KILPSKYNIC ENIEDGMLIY NTNSGGILKL NNEYKKKYDL FSKENFIEDT
ELKHALIHGK MIIPQTNVNE TEQLFLENKI ARFSGNMISL TIAPTMACNF RCPYCYEKGK
EYVTMDEKVI KKVKKYIQNL KDQYKYIHIT WYGGEPLLAF DIIKELMEEV YKNFDREFVS
SSAVSNGYLL NEAIALEMKT LNISHIQITI DGPPEIHNAR RRLPSGEDTF FVILNNLKKA
LDIYPDLDIS IRINVDKTNV SRINEIEQYL KEFNLFDKLS TYIAPVTNIN GTCTDGSCFT
VKEFALEEIN FIKKNYEADL SLISVPQKRV GMCGAVSLNS WLIDARGDFY KCWDDVGNQS
EKVGNIFQED FEINENLLKW LSYSIEEDEE CVQCPYLPVC MGGCPNYRIK NKGKDCHSIK
ENASQIVRLI YDISKQRLKN NV
//
