ID R9NDI7_9FIRM Unreviewed; 746 AA.
AC R9NDI7;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE RecName: Full=Alpha-galactosidase {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
DE EC=3.2.1.22 {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
GN ORFNames=C817_01002 {ECO:0000313|EMBL:EOS81345.1};
OS Dorea sp. 5-2.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae; Dorea.
OX NCBI_TaxID=1235798 {ECO:0000313|EMBL:EOS81345.1, ECO:0000313|Proteomes:UP000014211};
RN [1] {ECO:0000313|EMBL:EOS81345.1, ECO:0000313|Proteomes:UP000014211}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=5-2 {ECO:0000313|EMBL:EOS81345.1,
RC ECO:0000313|Proteomes:UP000014211};
RG The Broad Institute Genomics Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Earl A., Xavier R., Elson C., Duck W., Walker B., Young S., Zeng Q.,
RA Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Allen A.W., Alvarado L.,
RA Arachchi H.M., Berlin A.M., Chapman S.B., Gainer-Dewar J., Goldberg J.,
RA Griggs A., Gujja S., Hansen M., Howarth C., Imamovic A., Ireland A.,
RA Larimer J., McCowan C., Murphy C., Pearson M., Poon T.W., Priest M.,
RA Roberts A., Saif S., Shea T., Sisk P., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The Genome Sequence of Dorea bacterium 5-2.";
RL Submitted (APR-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing alpha-D-galactose
CC residues in alpha-D-galactosides, including galactose
CC oligosaccharides, galactomannans and galactolipids.; EC=3.2.1.22;
CC Evidence={ECO:0000256|ARBA:ARBA00001255,
CC ECO:0000256|PIRNR:PIRNR005536};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase.
CC {ECO:0000256|PIRNR:PIRNR005536}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EOS81345.1}.
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DR EMBL; ASTD01000018; EOS81345.1; -; Genomic_DNA.
DR RefSeq; WP_016217769.1; NZ_KE159726.1.
DR AlphaFoldDB; R9NDI7; -.
DR STRING; 1235798.C817_01002; -.
DR PATRIC; fig|1235798.3.peg.1074; -.
DR eggNOG; COG3345; Bacteria.
DR HOGENOM; CLU_009640_2_1_9; -.
DR OrthoDB; 9758822at2; -.
DR Proteomes; UP000014211; Unassembled WGS sequence.
DR GO; GO:0004557; F:alpha-galactosidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016052; P:carbohydrate catabolic process; IEA:InterPro.
DR CDD; cd14791; GH36; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR Gene3D; 2.70.98.60; alpha-galactosidase from lactobacil brevis; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR038417; Alpga-gal_N_sf.
DR InterPro; IPR000111; Glyco_hydro_27/36_CS.
DR InterPro; IPR002252; Glyco_hydro_36.
DR InterPro; IPR031705; Glyco_hydro_36_C.
DR InterPro; IPR031704; Glyco_hydro_36_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR43053:SF3; ALPHA-GALACTOSIDASE C-RELATED; 1.
DR PANTHER; PTHR43053; GLYCOSIDASE FAMILY 31; 1.
DR Pfam; PF16874; Glyco_hydro_36C; 1.
DR Pfam; PF16875; Glyco_hydro_36N; 1.
DR Pfam; PF02065; Melibiase; 1.
DR PIRSF; PIRSF005536; Agal; 1.
DR PRINTS; PR00743; GLHYDRLASE36.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR PROSITE; PS00512; ALPHA_GALACTOSIDASE; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PIRNR:PIRNR005536};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR005536};
KW Reference proteome {ECO:0000313|Proteomes:UP000014211}.
FT DOMAIN 30..296
FT /note="Glycosyl hydrolase family 36 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF16875"
FT DOMAIN 665..738
FT /note="Glycosyl hydrolase family 36 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF16874"
FT ACT_SITE 492
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
FT ACT_SITE 562
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
SQ SEQUENCE 746 AA; 85123 MW; EBE8606A01C20338 CRC64;
MAILFDSTSR LFTLTTAHSM YQMKADRYGV LLHTWFGMKG SAFDYSYRIA YADRGFSGNP
YDAGEDRTYS LDVLPQEFPV YGSGDYRSTA LKVRYPDGTT ACDLRYLKHE IREGKYSLPG
LPALFDTLPD AEDHASGLSI EAQSDTLVIT LEDTRRMFYV HLYYGVMENL DVITRAVCVE
NHSDADIYIE NIQSVCVDHL HGDHDLHTFY GRHVKERNYQ RTHVMHGIQS VGSTRGTSSH
QYNPFLILSD RDTTEDTGSC YGFSFLYSGD FIGSAELDQY DQTRFLLGIH PDNFCYCVKP
DECFYAPEAA MAYSECGLGN LSRIFHRAFR SHLCRGKWTV KRRPVLLNDW EGVYFDFDGE
KLIQMAKDAS ELGVELFVMD DGWFGKRDLD VSGLGDWIVN EKKLGCTLKE LSDQIHDFGI
QFGIWFEPEG INEDSDLYRE HPDWTFQIPG KQPTRSRLQL LLDFSREDVR DYIFTHICQV
LDSARIEYLK WDLNRSLADL YSAALPAERQ GEIMHRYVLG LYDMLEKLVR RYPDLLIEGC
SGGGGRFDAG MLYYTPQIWC SDDTDAIERL SIQYGTSFGY PVSTMGSHVS HCPNEQTRRI
APLNTRATVA MSGTFGYELD VNKMSGEEKE IVKAQIQEFK KYYDLIQYGD YYRLTDPDTV
EAYHGWEFAA SDGSEALLCI VALQIHPNGP GRWMCLKGLK KDSIYVVDGK EYPGDVLMNG
GLLLPGATEE YQSFRIHIVE KQKAGE
//
