ID RAN_CAEEL Reviewed; 215 AA.
AC O17915;
DT 24-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 27-MAR-2024, entry version 179.
DE RecName: Full=GTP-binding nuclear protein ran-1;
DE AltName: Full=Ras-related nuclear protein 1;
GN Name=ran-1; ORFNames=K01G5.4;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|EMBL:CAB07240.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=Bristol N2 {ECO:0000269|PubMed:11909538};
RX PubMed=11909538; DOI=10.1016/s0960-9822(02)00741-8;
RA Bamba C., Bobinnec Y., Fukuda M., Nishida E.;
RT "The GTPase Ran regulates chromosome positioning and nuclear envelope
RT assembly in vivo.";
RL Curr. Biol. 12:503-507(2002).
CC -!- FUNCTION: Ran GTPase system comprises ran-1, ran-2 and ran-3 and is
CC essential in nucleocytoplasmic transport. Ran-1 is a GTP-binding
CC protein that mediates the interaction between mitotic chromosomes and
CC kinetochore microtubules. Plays a crucial role in nuclear envelope
CC assembly at the end of each cell division. Required for the import of
CC protein into the nucleus and also for RNA export. RCC1 (ran-3)/Ran
CC (ran-1) complex (together with other proteins) acts as a component of a
CC signal transmission pathway that detects unreplicated DNA.
CC {ECO:0000269|PubMed:11909538}.
CC -!- SUBUNIT: Found in a nuclear export complex with RanGTP, exportin and
CC pre-miRNA (By similarity). {ECO:0000250|UniProtKB:P62825}.
CC -!- INTERACTION:
CC O17915; Q86G90: npp-9; NbExp=6; IntAct=EBI-324099, EBI-313076;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:11909538}.
CC Chromosome, centromere, kinetochore {ECO:0000269|PubMed:11909538}.
CC Chromosome {ECO:0000269|PubMed:11909538}. Note=At interphase, ran-1 is
CC localized to the nucleus and the nuclear envelope. At prometaphase,
CC ran-1 is still localized to the nuclear envelope. When the nuclear
CC envelope begins to disassemble at M phase (early metaphase), ran-1 is
CC localized throughout the entire cell. At late metaphase, ran-1
CC localizes to the kinetochore regions, this persists until anaphase. At
CC telophase, ran-1 is localized around the decondensing chromosomes.
CC -!- MISCELLANEOUS: Loss of ran-1 results in failure of correct attachment
CC of kinetochore microtubules to mitotic chromosomes and the resultant
CC loss of correct chromosome segregation. The number and the location of
CC centrosomes are normal, and astral mictrotubules also seem unaffected.
CC Nuclear envelope is not reestablished at the end of each cell division.
CC {ECO:0000269|PubMed:11909538}.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Ran family.
CC {ECO:0000305}.
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DR EMBL; Z92803; CAB07240.1; -; Genomic_DNA.
DR PIR; T23195; T23195.
DR RefSeq; NP_499369.1; NM_066968.5.
DR AlphaFoldDB; O17915; -.
DR SMR; O17915; -.
DR BioGRID; 41690; 57.
DR DIP; DIP-25217N; -.
DR IntAct; O17915; 15.
DR STRING; 6239.K01G5.4.1; -.
DR iPTMnet; O17915; -.
DR EPD; O17915; -.
DR PaxDb; 6239-K01G5-4; -.
DR PeptideAtlas; O17915; -.
DR EnsemblMetazoa; K01G5.4.1; K01G5.4.1; WBGene00004302.
DR GeneID; 176503; -.
DR KEGG; cel:CELE_K01G5.4; -.
DR UCSC; K01G5.4.1; c. elegans.
DR AGR; WB:WBGene00004302; -.
DR WormBase; K01G5.4; CE16194; WBGene00004302; ran-1.
DR eggNOG; KOG0096; Eukaryota.
DR GeneTree; ENSGT00940000153786; -.
DR HOGENOM; CLU_041217_13_0_1; -.
DR InParanoid; O17915; -.
DR OMA; GEIKFDC; -.
DR OrthoDB; 5471873at2759; -.
DR PhylomeDB; O17915; -.
DR Reactome; R-CEL-1655829; Regulation of cholesterol biosynthesis by SREBP (SREBF).
DR Reactome; R-CEL-9615933; Postmitotic nuclear pore complex (NPC) reformation.
DR PRO; PR:O17915; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00004302; Expressed in germ line (C elegans) and 4 other cell types or tissues.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0000776; C:kinetochore; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0009792; P:embryo development ending in birth or egg hatching; IMP:WormBase.
DR GO; GO:0048477; P:oogenesis; IMP:WormBase.
DR GO; GO:0006606; P:protein import into nucleus; IBA:GO_Central.
DR GO; GO:0000054; P:ribosomal subunit export from nucleus; IBA:GO_Central.
DR CDD; cd00877; Ran; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR002041; Ran_GTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR NCBIfam; TIGR00231; small_GTP; 1.
DR PANTHER; PTHR24071:SF0; GTP-BINDING NUCLEAR PROTEIN RAN; 1.
DR PANTHER; PTHR24071; RAN GTPASE; 1.
DR Pfam; PF00071; Ras; 1.
DR PRINTS; PR00627; GTPRANTC4.
DR SMART; SM00175; RAB; 1.
DR SMART; SM00176; RAN; 1.
DR SMART; SM00173; RAS; 1.
DR SMART; SM00174; RHO; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51418; RAN; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; GTP-binding;
KW Kinetochore; Mitosis; Nucleotide-binding; Nucleus; Protein transport;
KW Reference proteome; Transport.
FT CHAIN 1..215
FT /note="GTP-binding nuclear protein ran-1"
FT /id="PRO_0000208708"
FT BINDING 17..24
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P62825"
FT BINDING 67
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P62825"
FT BINDING 121..124
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P62825"
FT BINDING 149..151
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P62825"
SQ SEQUENCE 215 AA; 24254 MW; 90B32613588745E5 CRC64;
MSGGDGIPTF KLVLVGDGGT GKTTFVKRHL TGEFEKKYVA TLGVEVHPLV FHTNRGQIRF
NVWDTAGQEK FGGLRDGYYI QGQCAIIMFD VTARVTYKNV PNWHRDLARV CENIPIVLCG
NKVDVKDRKV KAKTITFHRK KNLQYYDISA KSNYNFEKPF LWLARKLLGD PNLEFVAMPA
LAPPEVQMDP AMIAEYEKDL DNAAKADLPD DDDDL
//
