ID RL18B_SCHPO Reviewed; 187 AA.
AC Q8TFH1;
DT 31-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 27-MAR-2024, entry version 125.
DE RecName: Full=Large ribosomal subunit protein eL18B {ECO:0000305};
DE AltName: Full=60S ribosomal protein L18-B;
GN Name=rpl1802; Synonyms=rpl18b; ORFNames=SPAPB17E12.13;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-134 AND SER-136, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=18257517; DOI=10.1021/pr7006335;
RA Wilson-Grady J.T., Villen J., Gygi S.P.;
RT "Phosphoproteome analysis of fission yeast.";
RL J. Proteome Res. 7:1088-1097(2008).
CC -!- FUNCTION: Component of the ribosome, a large ribonucleoprotein complex
CC responsible for the synthesis of proteins in the cell. The small
CC ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the
CC encoded message by selecting cognate aminoacyl-transfer RNA (tRNA)
CC molecules. The large subunit (LSU) contains the ribosomal catalytic
CC site termed the peptidyl transferase center (PTC), which catalyzes the
CC formation of peptide bonds, thereby polymerizing the amino acids
CC delivered by tRNAs into a polypeptide chain. The nascent polypeptides
CC leave the ribosome through a tunnel in the LSU and interact with
CC protein factors that function in enzymatic processing, targeting, and
CC the membrane insertion of nascent chains at the exit of the ribosomal
CC tunnel. {ECO:0000250|UniProtKB:P0CX50}.
CC -!- SUBUNIT: Component of the small ribosomal subunit (SSU). Mature yeast
CC ribosomes consist of a small (40S) and a large (60S) subunit. The 40S
CC small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and at
CC least 33 different proteins. The large 60S subunit contains 3 rRNA
CC molecules (25S, 5.8S and 5S rRNA) and at least 46 different proteins.
CC eL18 interacts with NAP1. {ECO:0000250|UniProtKB:P0CX50}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}.
CC -!- MISCELLANEOUS: There are 2 genes for eL18 in S.pombe.
CC -!- SIMILARITY: Belongs to the eukaryotic ribosomal protein eL18 family.
CC {ECO:0000305}.
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DR EMBL; CU329670; CAD27506.1; -; Genomic_DNA.
DR RefSeq; NP_001018228.1; NM_001018708.2.
DR AlphaFoldDB; Q8TFH1; -.
DR SMR; Q8TFH1; -.
DR BioGRID; 280453; 9.
DR IntAct; Q8TFH1; 1.
DR STRING; 284812.Q8TFH1; -.
DR iPTMnet; Q8TFH1; -.
DR MaxQB; Q8TFH1; -.
DR PaxDb; 4896-SPAPB17E12-13-1; -.
DR EnsemblFungi; SPAPB17E12.13.1; SPAPB17E12.13.1:pep; SPAPB17E12.13.
DR GeneID; 3361377; -.
DR KEGG; spo:SPAPB17E12.13; -.
DR PomBase; SPAPB17E12.13; rpl1802.
DR VEuPathDB; FungiDB:SPAPB17E12.13; -.
DR eggNOG; KOG1714; Eukaryota.
DR HOGENOM; CLU_080024_0_1_1; -.
DR InParanoid; Q8TFH1; -.
DR OMA; DHTTKQH; -.
DR PhylomeDB; Q8TFH1; -.
DR Reactome; R-SPO-156827; L13a-mediated translational silencing of Ceruloplasmin expression.
DR Reactome; R-SPO-1799339; SRP-dependent cotranslational protein targeting to membrane.
DR Reactome; R-SPO-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR Reactome; R-SPO-72689; Formation of a pool of free 40S subunits.
DR Reactome; R-SPO-72706; GTP hydrolysis and joining of the 60S ribosomal subunit.
DR Reactome; R-SPO-975956; Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
DR Reactome; R-SPO-975957; Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
DR PRO; PR:Q8TFH1; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0022625; C:cytosolic large ribosomal subunit; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003735; F:structural constituent of ribosome; IBA:GO_Central.
DR GO; GO:0002181; P:cytoplasmic translation; ISO:PomBase.
DR Gene3D; 3.100.10.10; -; 1.
DR InterPro; IPR000039; Ribosomal_eL18.
DR InterPro; IPR021132; Ribosomal_eL18/eL18-A/B/_CS.
DR InterPro; IPR021131; Ribosomal_uL15/eL18.
DR InterPro; IPR036227; Ribosomal_uL15/eL18_sf.
DR PANTHER; PTHR10934; 60S RIBOSOMAL PROTEIN L18; 1.
DR PANTHER; PTHR10934:SF2; 60S RIBOSOMAL PROTEIN L18; 1.
DR Pfam; PF17135; Ribosomal_L18; 1.
DR SUPFAM; SSF52080; Ribosomal proteins L15p and L18e; 1.
DR PROSITE; PS01106; RIBOSOMAL_L18E; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Phosphoprotein; Reference proteome; Ribonucleoprotein;
KW Ribosomal protein.
FT CHAIN 1..187
FT /note="Large ribosomal subunit protein eL18B"
FT /id="PRO_0000132783"
FT MOD_RES 134
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 136
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
SQ SEQUENCE 187 AA; 21184 MW; 426C5BF1388B2BDF CRC64;
MGIDIERHHV RKSQRSKPAS ENVYLKLLVK LYRFLARRTD SRFNKAILKR LFQSKTNRPP
ISISKIAALT SRKSASLEGK TTVIVGTVTD DERLLTVPKL SVAALRFTKS ARARILKAGG
EVLTLDQLAL RAPTGSNTVL LRGKKHAREA YRHFGFGPHK HKAPYVRSEG RKFERARGRR
KSRAFKV
//
