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Database: UniProt
Entry: RM13_YEAST
LinkDB: RM13_YEAST
Original site: RM13_YEAST 
ID   RM13_YEAST              Reviewed;         264 AA.
AC   Q02204; D6VX72;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2006, sequence version 2.
DT   20-JUN-2018, entry version 148.
DE   RecName: Full=54S ribosomal protein L13, mitochondrial;
DE   AltName: Full=Mitochondrial large ribosomal subunit protein mL50 {ECO:0000303|PubMed:24675956};
DE   AltName: Full=YmL13;
DE   Flags: Precursor;
GN   Name=MRPL13; OrderedLocusNames=YKR006C; ORFNames=YK105;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=07173;
RX   PubMed=7954901; DOI=10.1007/BF00326298;
RA   Grohmann L., Kitakawa M., Isono K., Goldschmidt-Reisin S.,
RA   Graack H.-R.;
RT   "The yeast nuclear gene MRP-L13 codes for a protein of the large
RT   subunit of the mitochondrial ribosome.";
RL   Curr. Genet. 26:8-14(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=1441752; DOI=10.1002/yea.320080908;
RA   Duesterhoeft A., Philippsen P.;
RT   "DNA sequencing and analysis of a 24.7 kb segment encompassing
RT   centromere CEN11 of Saccharomyces cerevisiae reveals nine previously
RT   unknown open reading frames.";
RL   Yeast 8:749-759(1992).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8196765; DOI=10.1038/369371a0;
RA   Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA   Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M.,
RA   Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA   Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA   Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA   Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA   Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA   Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA   Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA   Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA   Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA   Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA   Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA   Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA   Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA   Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA   Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA   Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA   Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA   van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C.,
RA   Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G.,
RA   Zimmermann J., Haasemann M., Becker I., Mewes H.-W.;
RT   "Complete DNA sequence of yeast chromosome XI.";
RL   Nature 369:371-378(1994).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M.,
RA   Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and
RT   now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [5]
RP   PROTEIN SEQUENCE OF 76-109, AND SUBUNIT.
RC   STRAIN=07173;
RX   PubMed=2060626; DOI=10.1016/0014-5793(91)80759-V;
RA   Grohmann L., Graack H.-R., Kruft V., Choli T., Goldschmidt-Reisin S.,
RA   Kitakawa M.;
RT   "Extended N-terminal sequencing of proteins of the large ribosomal
RT   subunit from yeast mitochondria.";
RL   FEBS Lett. 284:51-56(1991).
RN   [6]
RP   TRANSPORT INTO MITOCHONDRIA.
RX   PubMed=8220240;
RA   Matsushita Y., Isono K.;
RT   "Transport of mitoribosomal proteins, YmL13 and MRP7, into isolated
RT   mitochondria of Saccharomyces cerevisiae.";
RL   Biochem. Mol. Biol. Int. 30:911-919(1993).
RN   [7]
RP   IDENTIFICATION OF PROBABLE INITIATION SITE.
RX   PubMed=12748633; DOI=10.1038/nature01644;
RA   Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.;
RT   "Sequencing and comparison of yeast species to identify genes and
RT   regulatory elements.";
RL   Nature 423:241-254(2003).
RN   [8]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [9]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
RA   Dephoure N., O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [10]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 76625 / YPH499;
RX   PubMed=14576278; DOI=10.1073/pnas.2135385100;
RA   Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P.,
RA   Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B.,
RA   Rehling P., Pfanner N., Meisinger C.;
RT   "The proteome of Saccharomyces cerevisiae mitochondria.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003).
RN   [11]
RP   SUBCELLULAR LOCATION.
RX   PubMed=25609543; DOI=10.1038/ncomms7019;
RA   Pfeffer S., Woellhaf M.W., Herrmann J.M., Forster F.;
RT   "Organization of the mitochondrial translation machinery studied in
RT   situ by cryoelectron tomography.";
RL   Nat. Commun. 6:6019-6019(2015).
RN   [12]
RP   STRUCTURE BY ELECTRON MICROSCOPY (3.20 ANGSTROMS), AND SUBUNIT.
RX   PubMed=24675956; DOI=10.1126/science.1249410;
RA   Amunts A., Brown A., Bai X.C., Llacer J.L., Hussain T., Emsley P.,
RA   Long F., Murshudov G., Scheres S.H., Ramakrishnan V.;
RT   "Structure of the yeast mitochondrial large ribosomal subunit.";
RL   Science 343:1485-1489(2014).
CC   -!- FUNCTION: Component of the mitochondrial ribosome (mitoribosome),
CC       a dedicated translation machinery responsible for the synthesis of
CC       mitochondrial genome-encoded proteins, including at least some of
CC       the essential transmembrane subunits of the mitochondrial
CC       respiratory chain. The mitoribosomes are attached to the
CC       mitochondrial inner membrane and translation products are
CC       cotranslationally integrated into the membrane.
CC       {ECO:0000305|PubMed:24675956, ECO:0000305|PubMed:25609543}.
CC   -!- SUBUNIT: Component of the mitochondrial large ribosomal subunit
CC       (mt-LSU). Mature yeast 74S mitochondrial ribosomes consist of a
CC       small (37S) and a large (54S) subunit. The 37S small subunit
CC       contains a 15S ribosomal RNA (15S mt-rRNA) and 34 different
CC       proteins. The 54S large subunit contains a 21S rRNA (21S mt-rRNA)
CC       and 46 different proteins. {ECO:0000269|PubMed:2060626,
CC       ECO:0000269|PubMed:24675956}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:14562095,
CC       ECO:0000269|PubMed:14576278}. Note=Mitoribosomes are tethered to
CC       the mitochondrial inner membrane and spatially aligned with the
CC       membrane insertion machinery through two distinct membrane contact
CC       sites, formed by the 21S rRNA expansion segment 96-ES1 and the
CC       inner membrane protein MBA1. {ECO:0000269|PubMed:25609543}.
CC   -!- MISCELLANEOUS: Present with 1350 molecules/cell in log phase SD
CC       medium. {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the mitochondrion-specific ribosomal
CC       protein mL50 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA46246.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=CAA52022.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=CAA82076.1; Type=Erroneous initiation; Evidence={ECO:0000305};
DR   EMBL; X73673; CAA52022.1; ALT_INIT; Genomic_DNA.
DR   EMBL; X65124; CAA46246.1; ALT_INIT; Genomic_DNA.
DR   EMBL; Z28231; CAA82076.1; ALT_INIT; Genomic_DNA.
DR   EMBL; BK006944; DAA09162.1; -; Genomic_DNA.
DR   PIR; S25816; S25816.
DR   RefSeq; NP_012931.4; NM_001179796.3.
DR   PDB; 3J6B; EM; 3.20 A; 8=1-264.
DR   PDB; 5MRC; EM; 3.25 A; 8=1-264.
DR   PDB; 5MRE; EM; 3.75 A; 8=1-264.
DR   PDB; 5MRF; EM; 4.97 A; 8=1-264.
DR   PDBsum; 3J6B; -.
DR   PDBsum; 5MRC; -.
DR   PDBsum; 5MRE; -.
DR   PDBsum; 5MRF; -.
DR   ProteinModelPortal; Q02204; -.
DR   SMR; Q02204; -.
DR   BioGrid; 34138; 64.
DR   ComplexPortal; CPX-1602; 54S mitochondrial large ribosomal subunit.
DR   DIP; DIP-2092N; -.
DR   IntAct; Q02204; 7.
DR   MINT; Q02204; -.
DR   STRING; 4932.YKR006C; -.
DR   MaxQB; Q02204; -.
DR   PaxDb; Q02204; -.
DR   PRIDE; Q02204; -.
DR   EnsemblFungi; YKR006C; YKR006C; YKR006C.
DR   GeneID; 853875; -.
DR   KEGG; sce:YKR006C; -.
DR   EuPathDB; FungiDB:YKR006C; -.
DR   SGD; S000001714; MRPL13.
DR   HOGENOM; HOG000001090; -.
DR   InParanoid; Q02204; -.
DR   OMA; CARAPAV; -.
DR   OrthoDB; EOG092C3W5V; -.
DR   BioCyc; YEAST:G3O-31984-MONOMER; -.
DR   PRO; PR:Q02204; -.
DR   Proteomes; UP000002311; Chromosome XI.
DR   GO; GO:0005762; C:mitochondrial large ribosomal subunit; IDA:SGD.
DR   GO; GO:0003735; F:structural constituent of ribosome; IDA:SGD.
DR   GO; GO:0032543; P:mitochondrial translation; IC:SGD.
DR   Gene3D; 1.10.1200.10; -; 1.
DR   InterPro; IPR036736; ACP-like_sf.
DR   InterPro; IPR018305; Ribosomal_L50_mt.
DR   Pfam; PF10501; Ribosomal_L50; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Complete proteome; Direct protein sequencing;
KW   Mitochondrion; Reference proteome; Ribonucleoprotein;
KW   Ribosomal protein; Transit peptide.
FT   TRANSIT       1     75       Mitochondrion.
FT                                {ECO:0000269|PubMed:2060626,
FT                                ECO:0000269|PubMed:7954901}.
FT   CHAIN        76    264       54S ribosomal protein L13, mitochondrial.
FT                                /FTId=PRO_0000030571.
FT   CONFLICT    108    108       A -> S (in Ref. 2; CAA52022).
FT                                {ECO:0000305}.
SQ   SEQUENCE   264 AA;  30271 MW;  6418F9BD92910DDA CRC64;
     MSSLLKLHCI RPLPQRSVWL SGYKQKARCI HSSAANGDFM SWFKRKKQEE HQEPVKDTKQ
     LIKDIEEGTN EASSQSSSNN KNRLELIPEN FIGEGSRRCK RQKELKLAVS SAPFNQWLSR
     DKITSDNQLD DMILQATEKT LGKVDQDVQF SDLVAKFQFT KFLQSKSGYL IPDYELTTLS
     TPLQFKRYIK EKILPSANDP KLAYKEAEPN AIHPFSDNYA SPNIYVVNDV TSKEQKSKYD
     TIMKEIQKLE DDATRKALET ARSA
//
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