ID RUVA_UREPA Reviewed; 195 AA.
AC Q9PQ41;
DT 02-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 27-MAR-2024, entry version 105.
DE RecName: Full=Holliday junction branch migration complex subunit RuvA {ECO:0000255|HAMAP-Rule:MF_00031};
GN Name=ruvA {ECO:0000255|HAMAP-Rule:MF_00031}; OrderedLocusNames=UU449;
OS Ureaplasma parvum serovar 3 (strain ATCC 700970).
OC Bacteria; Mycoplasmatota; Mycoplasmoidales; Mycoplasmoidaceae; Ureaplasma.
OX NCBI_TaxID=273119;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700970;
RX PubMed=11048724; DOI=10.1038/35037619;
RA Glass J.I., Lefkowitz E.J., Glass J.S., Heiner C.R., Chen E.Y.,
RA Cassell G.H.;
RT "The complete sequence of the mucosal pathogen Ureaplasma urealyticum.";
RL Nature 407:757-762(2000).
CC -!- FUNCTION: The RuvA-RuvB-RuvC complex processes Holliday junction (HJ)
CC DNA during genetic recombination and DNA repair, while the RuvA-RuvB
CC complex plays an important role in the rescue of blocked DNA
CC replication forks via replication fork reversal (RFR). RuvA
CC specifically binds to HJ cruciform DNA, conferring on it an open
CC structure. The RuvB hexamer acts as an ATP-dependent pump, pulling
CC dsDNA into and through the RuvAB complex. HJ branch migration allows
CC RuvC to scan DNA until it finds its consensus sequence, where it
CC cleaves and resolves the cruciform DNA. {ECO:0000255|HAMAP-
CC Rule:MF_00031}.
CC -!- SUBUNIT: Homotetramer. Forms an RuvA(8)-RuvB(12)-Holliday junction (HJ)
CC complex. HJ DNA is sandwiched between 2 RuvA tetramers; dsDNA enters
CC through RuvA and exits via RuvB. An RuvB hexamer assembles on each DNA
CC strand where it exits the tetramer. Each RuvB hexamer is contacted by
CC two RuvA subunits (via domain III) on 2 adjacent RuvB subunits; this
CC complex drives branch migration. In the full resolvosome a probable
CC DNA-RuvA(4)-RuvB(12)-RuvC(2) complex forms which resolves the HJ.
CC {ECO:0000255|HAMAP-Rule:MF_00031}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00031}.
CC -!- DOMAIN: Has three domains with a flexible linker between the domains II
CC and III and assumes an 'L' shape. Domain III is highly mobile and
CC contacts RuvB. {ECO:0000255|HAMAP-Rule:MF_00031}.
CC -!- SIMILARITY: Belongs to the RuvA family. {ECO:0000255|HAMAP-
CC Rule:MF_00031}.
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DR EMBL; AF222894; AAF30861.1; -; Genomic_DNA.
DR RefSeq; WP_006688612.1; NC_002162.1.
DR AlphaFoldDB; Q9PQ41; -.
DR SMR; Q9PQ41; -.
DR STRING; 273119.UU449; -.
DR EnsemblBacteria; AAF30861; AAF30861; UU449.
DR GeneID; 29672569; -.
DR KEGG; uur:UU449; -.
DR eggNOG; COG0632; Bacteria.
DR HOGENOM; CLU_087936_1_1_14; -.
DR OrthoDB; 5293449at2; -.
DR Proteomes; UP000000423; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0009379; C:Holliday junction helicase complex; IEA:InterPro.
DR GO; GO:0048476; C:Holliday junction resolvase complex; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0000400; F:four-way junction DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0009378; F:four-way junction helicase activity; IEA:InterPro.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR CDD; cd14332; UBA_RuvA_C; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR HAMAP; MF_00031; DNA_HJ_migration_RuvA; 1.
DR InterPro; IPR000085; RuvA.
DR InterPro; IPR010994; RuvA_2-like.
DR InterPro; IPR011114; RuvA_C.
DR NCBIfam; TIGR00084; ruvA; 1.
DR Pfam; PF14520; HHH_5; 1.
DR Pfam; PF07499; RuvA_C; 1.
DR SUPFAM; SSF47781; RuvA domain 2-like; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA damage; DNA recombination; DNA repair; DNA-binding;
KW Reference proteome.
FT CHAIN 1..195
FT /note="Holliday junction branch migration complex subunit
FT RuvA"
FT /id="PRO_0000094705"
FT REGION 1..66
FT /note="Domain I"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00031"
FT REGION 67..141
FT /note="Domain II"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00031"
FT REGION 141..195
FT /note="Domain III"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00031"
FT REGION 141
FT /note="Flexible linker"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00031"
SQ SEQUENCE 195 AA; 22727 MW; 70550A47F344C53B CRC64;
MNYLIFKVIY ANANIVVGEH NFIGYQIRIP KDYELEINKF CKLYLYEYIS IMPNKNLIIK
DLYGFRTYNE RLLFIDLISI NSIGPKTAIN ILKYDIDTII DAIATKNIDY LITIKGINQR
NANLICDQLN YKYINKVNDK NNWAKELSIG LENLGYTKKD IEYAITKVKI NSQQDIDISE
IISSAIKEIS LRHEN
//
