ID S0JBJ4_9ENTE Unreviewed; 534 AA.
AC S0JBJ4;
DT 18-SEP-2013, integrated into UniProtKB/TrEMBL.
DT 18-SEP-2013, sequence version 1.
DT 27-MAR-2024, entry version 47.
DE RecName: Full=Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex {ECO:0000256|RuleBase:RU003423};
DE EC=2.3.1.- {ECO:0000256|RuleBase:RU003423};
GN ORFNames=OMQ_01045 {ECO:0000313|EMBL:EOT29732.1};
OS Enterococcus saccharolyticus subsp. saccharolyticus ATCC 43076.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
OC Enterococcus.
OX NCBI_TaxID=1139996 {ECO:0000313|EMBL:EOT29732.1, ECO:0000313|Proteomes:UP000014136};
RN [1] {ECO:0000313|EMBL:EOT29732.1, ECO:0000313|Proteomes:UP000014136}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43076 {ECO:0000313|EMBL:EOT29732.1,
RC ECO:0000313|Proteomes:UP000014136};
RG The Broad Institute Genomics Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Earl A., Russ C., Gilmore M., Surin D., Walker B., Young S., Zeng Q.,
RA Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Allen A.W., Alvarado L.,
RA Arachchi H.M., Berlin A.M., Chapman S.B., Gainer-Dewar J., Goldberg J.,
RA Griggs A., Gujja S., Hansen M., Howarth C., Imamovic A., Ireland A.,
RA Larimer J., McCowan C., Murphy C., Pearson M., Poon T.W., Priest M.,
RA Roberts A., Saif S., Shea T., Sisk P., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The Genome Sequence of Enterococcus saccharolyticus ATCC_43076 (Illumina
RT only assembly).";
RL Submitted (MAR-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=(R)-lipoate; Xref=ChEBI:CHEBI:83088;
CC Evidence={ECO:0000256|ARBA:ARBA00001938,
CC ECO:0000256|RuleBase:RU003423};
CC -!- SIMILARITY: Belongs to the 2-oxoacid dehydrogenase family.
CC {ECO:0000256|ARBA:ARBA00007317, ECO:0000256|RuleBase:RU003423}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EOT29732.1}.
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DR EMBL; AHYT01000003; EOT29732.1; -; Genomic_DNA.
DR RefSeq; WP_016174843.1; NZ_KE136523.1.
DR AlphaFoldDB; S0JBJ4; -.
DR STRING; 41997.RV16_GL002190; -.
DR PATRIC; fig|1139996.3.peg.1029; -.
DR eggNOG; COG0508; Bacteria.
DR HOGENOM; CLU_016733_10_0_9; -.
DR OrthoDB; 9805770at2; -.
DR Proteomes; UP000014136; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR GO; GO:0016746; F:acyltransferase activity; IEA:UniProtKB-KW.
DR CDD; cd06849; lipoyl_domain; 2.
DR Gene3D; 2.40.50.100; -; 2.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 1.
DR Gene3D; 4.10.320.10; E3-binding domain; 1.
DR InterPro; IPR003016; 2-oxoA_DH_lipoyl-BS.
DR InterPro; IPR001078; 2-oxoacid_DH_actylTfrase.
DR InterPro; IPR000089; Biotin_lipoyl.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR036625; E3-bd_dom_sf.
DR InterPro; IPR004167; PSBD.
DR InterPro; IPR011053; Single_hybrid_motif.
DR PANTHER; PTHR43178; DIHYDROLIPOAMIDE ACETYLTRANSFERASE COMPONENT OF PYRUVATE DEHYDROGENASE COMPLEX; 1.
DR PANTHER; PTHR43178:SF5; LIPOAMIDE ACYLTRANSFERASE COMPONENT OF BRANCHED-CHAIN ALPHA-KETO ACID DEHYDROGENASE COMPLEX, MITOCHONDRIAL; 1.
DR Pfam; PF00198; 2-oxoacid_dh; 1.
DR Pfam; PF00364; Biotin_lipoyl; 2.
DR Pfam; PF02817; E3_binding; 1.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 1.
DR SUPFAM; SSF47005; Peripheral subunit-binding domain of 2-oxo acid dehydrogenase complex; 1.
DR SUPFAM; SSF51230; Single hybrid motif; 2.
DR PROSITE; PS50968; BIOTINYL_LIPOYL; 2.
DR PROSITE; PS00189; LIPOYL; 2.
DR PROSITE; PS51826; PSBD; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315,
KW ECO:0000256|RuleBase:RU003423};
KW Lipoyl {ECO:0000256|ARBA:ARBA00022823, ECO:0000256|RuleBase:RU003423};
KW Reference proteome {ECO:0000313|Proteomes:UP000014136};
KW Transferase {ECO:0000256|RuleBase:RU003423, ECO:0000313|EMBL:EOT29732.1}.
FT DOMAIN 2..77
FT /note="Lipoyl-binding"
FT /evidence="ECO:0000259|PROSITE:PS50968"
FT DOMAIN 109..184
FT /note="Lipoyl-binding"
FT /evidence="ECO:0000259|PROSITE:PS50968"
FT DOMAIN 222..259
FT /note="Peripheral subunit-binding (PSBD)"
FT /evidence="ECO:0000259|PROSITE:PS51826"
FT REGION 190..220
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 273..303
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 534 AA; 56180 MW; 625D894BA6BBEB19 CRC64;
MAFQFKLPDI GEGIAEGEIV KWFVKPGDTI NEDDTLLEVQ NDKSVEEIPS PVTGTVKSIL
VEEGVVANVG DVLVEIDAPG YEDEAPAAPA QEQTPAAPAA LPEAGGQGVF QFKLPDIGEG
IAEGEIVKWF VKAGDTINED DTLLEVQNDK SVEEIPSPVT GTVVRVVVAE GVVANVGDVL
VEIDAPGHNN APAAAPAQEA AAPASAPAEA AAPTGKPSKN VLAMPSVRQF ARENDVDITL
VTPSGKGGRV TKEDIQSFIS GGSVAPAAPA QEAAAPVAEA KPAAKEEKAA PAKPYSSDLG
ALETREKLTP MRKAISKAMV NSKHTAPHVT LHDEVEVSKL WDQRKKFKDV AAAQNTKLTF
LPYVVKALTA TMKKYPVLNA SIDDAAQEVV YKNYYNIGIA TDTANGLYVP NVKDADRKGL
FAIADEINGK AALAHEGKLA AEDMRNGTVT ISNIGSVGGA WFTPVINYPE VAILGVGTIA
QQPVVNAEGE IVVGRMMKLS LSFDHRIVDG ATAQQAMNNI KRLLADPELL LMEG
//
