ID S0KJM7_9ENTE Unreviewed; 579 AA.
AC S0KJM7;
DT 18-SEP-2013, integrated into UniProtKB/TrEMBL.
DT 18-SEP-2013, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=I568_00692 {ECO:0000313|EMBL:EOW84205.1};
OS Enterococcus columbae DSM 7374 = ATCC 51263.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
OC Enterococcus.
OX NCBI_TaxID=1121865 {ECO:0000313|EMBL:EOW84205.1, ECO:0000313|Proteomes:UP000014113};
RN [1] {ECO:0000313|EMBL:EOW84205.1, ECO:0000313|Proteomes:UP000014113}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51263 {ECO:0000313|EMBL:EOW84205.1,
RC ECO:0000313|Proteomes:UP000014113};
RG The Broad Institute Genomics Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Earl A., Russ C., Gilmore M., Surin D., Walker B., Young S., Zeng Q.,
RA Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Allen A.W., Alvarado L.,
RA Arachchi H.M., Berlin A.M., Chapman S.B., Gainer-Dewar J., Goldberg J.,
RA Griggs A., Gujja S., Hansen M., Howarth C., Imamovic A., Ireland A.,
RA Larimer J., McCowan C., Murphy C., Pearson M., Poon T.W., Priest M.,
RA Roberts A., Saif S., Shea T., Sisk P., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The Genome Sequence of Enterococcus columbae ATCC_51263 (PacBio/Illumina
RT hybrid assembly).";
RL Submitted (MAR-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EOW84205.1}.
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DR EMBL; ASWJ01000004; EOW84205.1; -; Genomic_DNA.
DR RefSeq; WP_016182269.1; NZ_KE136493.1.
DR AlphaFoldDB; S0KJM7; -.
DR STRING; 1121865.OMW_00098; -.
DR PATRIC; fig|1121865.3.peg.93; -.
DR eggNOG; COG0286; Bacteria.
DR eggNOG; COG0732; Bacteria.
DR OrthoDB; 9814572at2; -.
DR Proteomes; UP000014113; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0006306; P:DNA methylation; IEA:InterPro.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 3.90.220.20; DNA methylase specificity domains; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR044946; Restrct_endonuc_typeI_TRD_sf.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR PANTHER; PTHR42933:SF3; TYPE I RESTRICTION ENZYME MJAVIII METHYLASE SUBUNIT; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR SUPFAM; SSF116734; DNA methylase specificity domain; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE 4: Predicted;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000014113};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 118..383
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 579 AA; 66205 MW; 6C319DCB73E583AD CRC64;
MDIKDLKSRE YGNLSKYVSM IKYHLNSVFE EEVEDAKIFA TAFLAYLVKK ENLSTKRELI
NYIERKLPES QQLFLIDRTS DTYWTKVLEI AEELSLDALL ATVVWYPYSG FYNTKEIATP
ESIVKLAYSI LNPKNEYVAD LCSGVGSFLS YTAMQDDEST LYGVETKAYP KVISEIILSF
FENEAKIVQG SALEIPKNKK FDKIFCEYPW NLNSRYLSDD EKQAVLDDGL IPELAKTSRT
EWYFILNVVN HLSENGKAVV YAPNAITWDE DRLKIIRERF IKLGLLEAAI TIPKEIVSPY
AGVRSVLLVL SKNKSDSVRF IDASELELVD VNNRGLFYSY KDETIEEILT MLDSDGKYSK
KVSIDEIAKK DYVINPVRYL ESEIKVKNGV PFESLIKNIT RGIQMRAAGI EEMISDTPTP
YRYLKLTDIQ DGLINEDLPY LKEIDEKQKK YCLKNRSLVI SKTVNSLKVA VSNVEEGQQI
LVSGNLYIIE LDETKANPYY VKSYLESEQG TVALSRISVG SVIANIPISK LSKLMIPNPS
IAFQNKVAEK YLTKVDEIKL LRYRLQKATD DLKKIFEEE
//
