ID S0KN80_9ENTE Unreviewed; 380 AA.
AC S0KN80;
DT 18-SEP-2013, integrated into UniProtKB/TrEMBL.
DT 18-SEP-2013, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE SubName: Full=IS605 OrfB family transposase {ECO:0000313|EMBL:EOW87604.1};
GN ORFNames=I568_00269 {ECO:0000313|EMBL:EOW87604.1}, I568_00485
GN {ECO:0000313|EMBL:EOW87441.1};
OS Enterococcus columbae DSM 7374 = ATCC 51263.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
OC Enterococcus.
OX NCBI_TaxID=1121865 {ECO:0000313|EMBL:EOW87604.1, ECO:0000313|Proteomes:UP000014113};
RN [1] {ECO:0000313|EMBL:EOW87604.1, ECO:0000313|Proteomes:UP000014113}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51263 {ECO:0000313|EMBL:EOW87604.1,
RC ECO:0000313|Proteomes:UP000014113};
RG The Broad Institute Genomics Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Earl A., Russ C., Gilmore M., Surin D., Walker B., Young S., Zeng Q.,
RA Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Allen A.W., Alvarado L.,
RA Arachchi H.M., Berlin A.M., Chapman S.B., Gainer-Dewar J., Goldberg J.,
RA Griggs A., Gujja S., Hansen M., Howarth C., Imamovic A., Ireland A.,
RA Larimer J., McCowan C., Murphy C., Pearson M., Poon T.W., Priest M.,
RA Roberts A., Saif S., Shea T., Sisk P., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The Genome Sequence of Enterococcus columbae ATCC_51263 (PacBio/Illumina
RT hybrid assembly).";
RL Submitted (MAR-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: In the C-terminal section; belongs to the transposase 35
CC family. {ECO:0000256|ARBA:ARBA00008761}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the transposase 2
CC family. {ECO:0000256|ARBA:ARBA00011044}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EOW87604.1}.
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DR EMBL; ASWJ01000003; EOW87441.1; -; Genomic_DNA.
DR EMBL; ASWJ01000002; EOW87604.1; -; Genomic_DNA.
DR RefSeq; WP_016183099.1; NZ_KE136492.1.
DR AlphaFoldDB; S0KN80; -.
DR STRING; 1121865.OMW_00938; -.
DR PATRIC; fig|1121865.3.peg.925; -.
DR eggNOG; COG0675; Bacteria.
DR Proteomes; UP000014113; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0032196; P:transposition; IEA:UniProtKB-KW.
DR InterPro; IPR010095; Cas12f1-like_TNB.
DR InterPro; IPR001959; Transposase.
DR InterPro; IPR021027; Transposase_put_HTH.
DR NCBIfam; NF040570; guided_TnpB; 1.
DR NCBIfam; TIGR01766; IS200/IS605 family accessory protein TnpB-like domain; 1.
DR NCBIfam; NF038281; IS200_TnpB; 1.
DR PANTHER; PTHR30405:SF20; RNA-GUIDED DNA ENDONUCLEASE INSQ-RELATED; 1.
DR PANTHER; PTHR30405; TRANSPOSASE; 1.
DR Pfam; PF12323; HTH_OrfB_IS605; 1.
DR Pfam; PF01385; OrfB_IS605; 1.
DR Pfam; PF07282; OrfB_Zn_ribbon; 1.
PE 3: Inferred from homology;
KW DNA recombination {ECO:0000256|ARBA:ARBA00023172};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Endonuclease {ECO:0000256|ARBA:ARBA00022759};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Reference proteome {ECO:0000313|Proteomes:UP000014113};
KW Transposition {ECO:0000256|ARBA:ARBA00022578};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 1..45
FT /note="Transposase putative helix-turn-helix"
FT /evidence="ECO:0000259|Pfam:PF12323"
FT DOMAIN 171..290
FT /note="Probable transposase IS891/IS1136/IS1341"
FT /evidence="ECO:0000259|Pfam:PF01385"
FT DOMAIN 302..370
FT /note="Cas12f1-like TNB"
FT /evidence="ECO:0000259|Pfam:PF07282"
SQ SEQUENCE 380 AA; 44077 MW; 565BAF20B6DC3A85 CRC64;
MERLKAYKFR IYPTEEQEIF FAKTFGCVRK VYNLMLNDRK KAYEEVKNDP SKKMTFPTPA
KYKKEFPFLK EVDSLALANA QLHLDKAYKN FFRDKSVGFP RFKSKKNPVQ SYTTNNQNGT
VALIDSKFIK VPKLKSLVRI KLHRQPKGMI KSATISRHSS GKYYISLLCK EEISELPKTN
SAIGIDLGIT DFAILSDGQK IDNHKFTSKM EKKLKREQRK LSRRALLAKK KGIPLSEAKN
YQKQKRKVAR LHEKVMNQRT DFLNKLSTEI IKNHDIICIE DLNVKGMLRN HKLARSISDV
SWSSFVAKLQ YKADWYGREI IKVDTWFPSS QICSECGHKD GKKSLDIREW TCPICHTHHD
RDINASINIL IEGLRIQTSA
//
