ID   S1MTE8_9ENTE            Unreviewed;       470 AA.
AC   S1MTE8;
DT   18-SEP-2013, integrated into UniProtKB/TrEMBL.
DT   18-SEP-2013, sequence version 1.
DT   24-JAN-2024, entry version 31.
DE   SubName: Full=Uncharacterized protein {ECO:0000313|EMBL:EOW79920.1};
GN   ORFNames=I568_02271 {ECO:0000313|EMBL:EOW79920.1};
OS   Enterococcus columbae DSM 7374 = ATCC 51263.
OC   Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
OC   Enterococcus.
OX   NCBI_TaxID=1121865 {ECO:0000313|EMBL:EOW79920.1, ECO:0000313|Proteomes:UP000014113};
RN   [1] {ECO:0000313|EMBL:EOW79920.1, ECO:0000313|Proteomes:UP000014113}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51263 {ECO:0000313|EMBL:EOW79920.1,
RC   ECO:0000313|Proteomes:UP000014113};
RG   The Broad Institute Genomics Platform;
RG   The Broad Institute Genome Sequencing Center for Infectious Disease;
RA   Earl A., Russ C., Gilmore M., Surin D., Walker B., Young S., Zeng Q.,
RA   Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Allen A.W., Alvarado L.,
RA   Arachchi H.M., Berlin A.M., Chapman S.B., Gainer-Dewar J., Goldberg J.,
RA   Griggs A., Gujja S., Hansen M., Howarth C., Imamovic A., Ireland A.,
RA   Larimer J., McCowan C., Murphy C., Pearson M., Poon T.W., Priest M.,
RA   Roberts A., Saif S., Shea T., Sisk P., Sykes S., Wortman J., Nusbaum C.,
RA   Birren B.;
RT   "The Genome Sequence of Enterococcus columbae ATCC_51263 (PacBio/Illumina
RT   hybrid assembly).";
RL   Submitted (MAR-2013) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC       {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:EOW79920.1}.
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DR   EMBL; ASWJ01000011; EOW79920.1; -; Genomic_DNA.
DR   RefSeq; WP_016184130.1; NZ_KE136498.1.
DR   AlphaFoldDB; S1MTE8; -.
DR   STRING; 1121865.OMW_02024; -.
DR   PATRIC; fig|1121865.3.peg.1971; -.
DR   eggNOG; COG3940; Bacteria.
DR   OrthoDB; 177947at2; -.
DR   Proteomes; UP000014113; Unassembled WGS sequence.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd18820; GH43_LbAraf43-like; 1.
DR   Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR   InterPro; IPR006710; Glyco_hydro_43.
DR   InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR   PANTHER; PTHR43817; GLYCOSYL HYDROLASE; 1.
DR   PANTHER; PTHR43817:SF1; HYDROLASE, FAMILY 43, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G01660)-RELATED; 1.
DR   Pfam; PF04616; Glyco_hydro_43; 1.
DR   SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|RuleBase:RU361187};
KW   Hydrolase {ECO:0000256|RuleBase:RU361187};
KW   Reference proteome {ECO:0000313|Proteomes:UP000014113};
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..28
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           29..470
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5030177105"
FT   ACT_SITE        46
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT   ACT_SITE        214
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT   SITE            152
FT                   /note="Important for catalytic activity, responsible for
FT                   pKa modulation of the active site Glu and correct
FT                   orientation of both the proton donor and substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ   SEQUENCE   470 AA;  53788 MW;  3D7C46D314E851F6 CRC64;
     MKKKLLLGAI LTCGLVLGIA ACQFNQSAKF TMTKQMTNIV DNNGADPWVS YEKGRYYYTK
     TTGDSVTLWR SKQLETVFTG KQKTIFKIPD ELESIWAPEI HYLDGRWVVY FAANKANETH
     RMYALTNPSK DPYKGNWQLT PIKGMDDKFA IDGTILTVKD KNYFIWSGWQ GYENVAQNIY
     ISPMISPTEV ENKKIELSHP EYEWEMRQKP FINEGPQVTI KNDTIHLIYS ASGSWDNDYC
     LGELTAKVEA DLLNPASWSK AKAPILKSNA EVYGPGHHSI VSTKDGKQDW IIYHTARWDH
     SGWHRLVRMN QVKWQGNKIK LSSPTVDNEL ISRPSSQELS RFTLAATQAE KTKGLSVIND
     DYALNHQVIT GFENPNDQLT FHFQAPKTAE YTVMAYVKME NYDDPENILE AKFSVKQEEK
     IAKIYPSQYY QPVQIKIKLN QGKQTIHFQS VIGVDTLSVD RIEIIENQGE
//