ID S2LBM1_HALAF Unreviewed; 1171 AA.
AC S2LBM1;
DT 18-SEP-2013, integrated into UniProtKB/TrEMBL.
DT 18-SEP-2013, sequence version 1.
DT 27-MAR-2024, entry version 58.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
DE Flags: Fragment;
GN ORFNames=L861_05070 {ECO:0000313|EMBL:EPC02131.1};
OS Halomonas anticariensis (strain DSM 16096 / CECT 5854 / LMG 22089 / FP35).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Oceanospirillales;
OC Halomonadaceae; Halomonas.
OX NCBI_TaxID=1121939 {ECO:0000313|EMBL:EPC02131.1, ECO:0000313|Proteomes:UP000014463};
RN [1] {ECO:0000313|EMBL:EPC02131.1, ECO:0000313|Proteomes:UP000014463}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 16096 / CECT 5854 / LMG 22089 / FP35
RC {ECO:0000313|Proteomes:UP000014463};
RX PubMed=23868129; DOI=10.1128/genomeA.00497-13;
RA Tahrioui A., Quesada E., Llamas I.;
RT "Draft genome sequence of the moderately halophilic gammaproteobacterium
RT Halomonas anticariensis FP35.";
RL Genome Announc. 1:31-31(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EPC02131.1}.
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DR EMBL; ASTJ01000028; EPC02131.1; -; Genomic_DNA.
DR AlphaFoldDB; S2LBM1; -.
DR STRING; 1121939.L861_05070; -.
DR eggNOG; COG1074; Bacteria.
DR Proteomes; UP000014463; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0008854; F:exodeoxyribonuclease V activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:InterPro.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 1.10.3170.10; Recbcd, chain B, domain 2; 1.
DR HAMAP; MF_01485; RecB; 1.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR004586; RecB.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR NCBIfam; TIGR00609; recB; 1.
DR PANTHER; PTHR11070:SF23; RECBCD ENZYME SUBUNIT RECB; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF52980; Restriction endonuclease-like; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000014463}.
FT DOMAIN 1..487
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 525..799
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 93..116
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 22..29
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
FT NON_TER 1171
FT /evidence="ECO:0000313|EMBL:EPC02131.1"
SQ SEQUENCE 1171 AA; 129748 MW; C271C24A201C2D76 CRC64;
MSDVTQLNPL DFPLHGSRLI EASAGTGKTF TIALLYVRLV LGARHEGDEE AFARPLTPPE
ILVVTFTNAA TQELRERIRS RLVEAAGVFL QTAEAAPSPD GSPEAGRSGD PQGDPLLVAL
RDQYDPATWP ACARRLQLAA EWMDEAAVST IHSWCYRMLR EHAFDSGSLF SLDLETDQTE
LEHEVVRDYW RSFYYPLDDD ALATVVRHWD SPEKLHQAVR GLMPECEALD KHAPSPAETV
AQASHETTER LAELKAPWPA WVDECETLFE EAAKQKAFKG QSFNARSRAN WLGALREWCQ
SDAVWPGLTD AAWKRLTPQG MAEIWTKGEP PDHPALAALE ALQDELHALP DPYADLLTHA
VHWCRERLDR EQERRAEMGP NELLTHLDRA LAGPNGEALG AQIRRQFPVA LVDEFQDTDP
VQYRIFDGVY HIRENRRGYS ETRGVFLIGD PKQAIYAFRG ADIYTYLRAR RDTDGRHVTL
GTNFRSSQPM VTAVNRCFDY AEAHPSGAFL FKEDDGNNPV PFVGVNAKGR KDSLTANGQP
LPAMTLWQLE CDEPLAKGSY QQEMAERCAS HMVTLLEQGQ HGRAGFQGEG NDENAFTPLK
PSDMAVLVNG LGEARAIRQA LARRGVKSVY LSDKDKVFAS PVATQLDAWL RACAEPDDER
LLRTALATPV LGLDIATLDK LNEDELAWEA RVMQFRDYHR LWQGQGVLPL VRRLINDFGI
ATRLLAAGPA SDGERALTDL LHLGELLQQA SQELDGEHAL IRFLAESIAQ PEGQGDTHKL
RLESDADLVQ VVTIHKSKGL EYPLVFLPFI CNHRPTKRDD SPLRWHDAEG QLRISLEADD
ETLATVDRER LGEDLRKLYV ALTRARHATW LGMAPLKGGE GSAIGQLLAD GKALDPAGFA
NSLTQLKGDC TAIEIAPAPP ADAHVIRLDS TDEPLGEARM PTRPAREHWW IASYSALRIS
AGEVTAPVSA DTLHSAAFHS APTMAHTAPE SSPADTAHLP EPATSLEATA LEVITEPHDS
GHGEQIANLH RFPRGPAPGT FLHGLLEWAG EHGFGRVARD PALREDTLAR RCNLRGWQQW
LPTLHEWFGE LLSAPLPLPQ PQPSEAGEFR LEALTTYQVE MEFWFATTQV DTRRLDALVS
TYTLPGARST TPRPALDAET LNGMLKGFID L
//
