ID S2XEM2_9BACL Unreviewed; 600 AA.
AC S2XEM2;
DT 18-SEP-2013, integrated into UniProtKB/TrEMBL.
DT 18-SEP-2013, sequence version 1.
DT 27-MAR-2024, entry version 40.
DE SubName: Full=PepF/M3 family oligoendopeptidase {ECO:0000313|EMBL:EPD50206.1};
GN ORFNames=HMPREF1210_02777 {ECO:0000313|EMBL:EPD50206.1};
OS Paenisporosarcina sp. HGH0030.
OC Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
OC Paenisporosarcina.
OX NCBI_TaxID=1078085 {ECO:0000313|EMBL:EPD50206.1, ECO:0000313|Proteomes:UP000014412};
RN [1] {ECO:0000313|EMBL:EPD50206.1, ECO:0000313|Proteomes:UP000014412}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HGH0030 {ECO:0000313|EMBL:EPD50206.1,
RC ECO:0000313|Proteomes:UP000014412};
RG The Broad Institute Genomics Platform;
RA Earl A., Ward D., Feldgarden M., Gevers D., Schmidt T.M., Dai D., Dover J.,
RA Kim K., Walker B., Young S., Zeng Q., Gargeya S., Fitzgerald M., Haas B.,
RA Abouelleil A., Allen A.W., Alvarado L., Arachchi H.M., Berlin A.M.,
RA Chapman S.B., Gainer-Dewar J., Goldberg J., Griggs A., Gujja S., Hansen M.,
RA Howarth C., Imamovic A., Ireland A., Larimer J., McCowan C., Murphy C.,
RA Pearson M., Poon T.W., Priest M., Roberts A., Saif S., Shea T., Sisk P.,
RA Sykes S., Wortman J., Nusbaum C., Birren B.;
RT "The Genome Sequence of Paenisporosarcina sp. HGH0030.";
RL Submitted (MAY-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|RuleBase:RU003435};
CC Note=Binds 1 zinc ion. {ECO:0000256|RuleBase:RU003435};
CC -!- SIMILARITY: Belongs to the peptidase M3 family.
CC {ECO:0000256|RuleBase:RU003435}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EPD50206.1}.
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DR EMBL; AGEQ01000016; EPD50206.1; -; Genomic_DNA.
DR RefSeq; WP_016429263.1; NZ_KE150422.1.
DR AlphaFoldDB; S2XEM2; -.
DR STRING; 1078085.HMPREF1210_02777; -.
DR MEROPS; M03.A08; -.
DR PATRIC; fig|1078085.3.peg.2748; -.
DR eggNOG; COG1164; Bacteria.
DR HOGENOM; CLU_021290_3_1_9; -.
DR Proteomes; UP000014412; Unassembled WGS sequence.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004181; F:metallocarboxypeptidase activity; IEA:InterPro.
DR GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd09607; M3B_PepF; 1.
DR Gene3D; 1.10.1370.20; Oligoendopeptidase f, C-terminal domain; 1.
DR Gene3D; 1.20.140.70; Oligopeptidase f, N-terminal domain; 1.
DR InterPro; IPR034006; M3B_PepF_2.
DR InterPro; IPR013647; OligopepF_N_dom.
DR InterPro; IPR042088; OligoPept_F_C.
DR InterPro; IPR001567; Pept_M3A_M3B_dom.
DR InterPro; IPR011977; Pept_M3B_clade3.
DR InterPro; IPR001333; Peptidase_M32_Taq.
DR NCBIfam; TIGR02290; M3_fam_3; 1.
DR PANTHER; PTHR34217:SF1; CARBOXYPEPTIDASE 1; 1.
DR PANTHER; PTHR34217; METAL-DEPENDENT CARBOXYPEPTIDASE; 1.
DR Pfam; PF01432; Peptidase_M3; 1.
DR Pfam; PF08439; Peptidase_M3_N; 1.
DR SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|RuleBase:RU003435};
KW Metal-binding {ECO:0000256|RuleBase:RU003435};
KW Metalloprotease {ECO:0000256|RuleBase:RU003435};
KW Protease {ECO:0000256|RuleBase:RU003435};
KW Reference proteome {ECO:0000313|Proteomes:UP000014412};
KW Zinc {ECO:0000256|RuleBase:RU003435}.
FT DOMAIN 119..183
FT /note="Oligopeptidase F N-terminal"
FT /evidence="ECO:0000259|Pfam:PF08439"
FT DOMAIN 204..563
FT /note="Peptidase M3A/M3B catalytic"
FT /evidence="ECO:0000259|Pfam:PF01432"
SQ SEQUENCE 600 AA; 67815 MW; 28FD3284B9CA683F CRC64;
MTTKTYAEVW DLDVFFKGGS SSPELREHLD ALASRLDTFE QTLSSFVVPT GESDATKISS
IIEETKQVAE NLTQAGAVVG CYLAQDTTDK QANLLQGEIG SLGARFSSSM LVVQQQLSKT
EETVWTALMK DDQLKEFAFV LNEWRDEAKE LLSEKEEDMI TALGVDGYHG WGQLYDLLVG
DVKVRVTVDG EEKEFSVGQA SNLSSHPDAK VRKESFEALE QAWTDKEEFF AKTLNHMAGF
RLAVYKKRGW DSILKEPLQI NRMKQQTLDA MWGAISAHKA PFVEYLKRKA ELVGADAMHW
YDLDAPVTES TQKVSYQEGA EFILKHFGKF GPELEKFSRQ AFEDEWIEAE DRPNKRPGGF
CTGMPLSQQS RIFMTYSGTM SNVATLAHEL GHAFHSYALR PVHWMNRQYA MGVAETASTF
AEMIVADAAV KEASTADEKL ALLEDKIQRS VAFFMNIHAR FLFETRFYEE RKKGIVSAAR
LNTLMEEAQR EAFGDGLGEV HPHFWASKLH FYITHVPFYN FPYTFGYLFS LSIYAKAIEE
GCSFEEKYIS LLQDTAIMSV EDLAMKHLGE DITQHAFWEK GIALCIKDVE EFLSLTSSKG
//
