ID S3BWX5_OPHP1 Unreviewed; 1486 AA.
AC S3BWX5;
DT 18-SEP-2013, integrated into UniProtKB/TrEMBL.
DT 18-SEP-2013, sequence version 1.
DT 27-MAR-2024, entry version 42.
DE RecName: Full=5'-3' exoribonuclease 1 {ECO:0000256|PIRNR:PIRNR006743};
DE EC=3.1.13.- {ECO:0000256|PIRNR:PIRNR006743};
GN ORFNames=F503_08240 {ECO:0000313|EMBL:EPE05709.1};
OS Ophiostoma piceae (strain UAMH 11346) (Sap stain fungus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Ophiostomatales; Ophiostomataceae; Ophiostoma.
OX NCBI_TaxID=1262450 {ECO:0000313|EMBL:EPE05709.1, ECO:0000313|Proteomes:UP000016923};
RN [1] {ECO:0000313|EMBL:EPE05709.1, ECO:0000313|Proteomes:UP000016923}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UAMH 11346 {ECO:0000313|EMBL:EPE05709.1,
RC ECO:0000313|Proteomes:UP000016923};
RX PubMed=23725015; DOI=10.1186/1471-2164-14-373;
RA Haridas S., Wang Y., Lim L., Massoumi Alamouti S., Jackman S., Docking R.,
RA Robertson G., Birol I., Bohlmann J., Breuil C.;
RT "The genome and transcriptome of the pine saprophyte Ophiostoma piceae, and
RT a comparison with the bark beetle-associated pine pathogen Grosmannia
RT clavigera.";
RL BMC Genomics 14:373-373(2013).
CC -!- FUNCTION: Multifunctional protein that exhibits several independent
CC functions at different levels of the cellular processes. 5'-3'
CC exonuclease component of the nonsense-mediated mRNA decay (NMD) which
CC is a highly conserved mRNA degradation pathway, an RNA surveillance
CC system whose role is to identify and rid cells of mRNA with premature
CC termination codons and thus prevents accumulation of potentially
CC harmful truncated proteins. {ECO:0000256|PIRNR:PIRNR006743}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|PIRNR:PIRNR006743}.
CC -!- SIMILARITY: Belongs to the 5'-3' exonuclease family.
CC {ECO:0000256|PIRNR:PIRNR006743}.
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DR EMBL; KE148155; EPE05709.1; -; Genomic_DNA.
DR STRING; 1262450.S3BWX5; -.
DR VEuPathDB; FungiDB:F503_08240; -.
DR eggNOG; KOG2045; Eukaryota.
DR HOGENOM; CLU_001581_1_2_1; -.
DR OMA; VASWPWF; -.
DR OrthoDB; 167745at2759; -.
DR Proteomes; UP000016923; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004534; F:5'-3' RNA exonuclease activity; IEA:UniProt.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IEA:UniProtKB-KW.
DR CDD; cd18673; PIN_XRN1-2-like; 1.
DR Gene3D; 1.25.40.1050; -; 1.
DR Gene3D; 2.170.260.40; -; 1.
DR Gene3D; 2.30.30.30; -; 1.
DR Gene3D; 2.30.30.750; -; 1.
DR Gene3D; 3.40.50.12390; -; 2.
DR InterPro; IPR027073; 5_3_exoribonuclease.
DR InterPro; IPR016494; 5_3_exoribonuclease_1.
DR InterPro; IPR014722; Rib_uL2_dom2.
DR InterPro; IPR041385; SH3_12.
DR InterPro; IPR040992; XRN1_D1.
DR InterPro; IPR047007; XRN1_D1_sf.
DR InterPro; IPR041106; XRN1_D2_D3.
DR InterPro; IPR041412; Xrn1_helical.
DR InterPro; IPR004859; Xrn1_N.
DR InterPro; IPR047008; XRN1_SH3_sf.
DR PANTHER; PTHR12341:SF83; 5'-3' EXORIBONUCLEASE 1; 1.
DR PANTHER; PTHR12341; 5'->3' EXORIBONUCLEASE; 1.
DR Pfam; PF18129; SH3_12; 1.
DR Pfam; PF18332; XRN1_D1; 1.
DR Pfam; PF18334; XRN1_D2_D3; 1.
DR Pfam; PF17846; XRN_M; 1.
DR Pfam; PF03159; XRN_N; 1.
DR PIRSF; PIRSF006743; Exonuclease_Xnr1; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|PIRNR:PIRNR006743};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839, ECO:0000256|PIRNR:PIRNR006743};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR006743};
KW Nonsense-mediated mRNA decay {ECO:0000256|PIRNR:PIRNR006743};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722, ECO:0000256|PIRNR:PIRNR006743};
KW Reference proteome {ECO:0000313|Proteomes:UP000016923};
KW RNA-binding {ECO:0000256|PIRNR:PIRNR006743}.
FT DOMAIN 1..227
FT /note="Xrn1 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF03159"
FT DOMAIN 275..704
FT /note="Xrn1 helical"
FT /evidence="ECO:0000259|Pfam:PF17846"
FT DOMAIN 755..940
FT /note="5'-3' exoribonuclease 1 D1"
FT /evidence="ECO:0000259|Pfam:PF18332"
FT DOMAIN 944..1169
FT /note="Exoribonuclease Xrn1 D2/D3"
FT /evidence="ECO:0000259|Pfam:PF18334"
FT DOMAIN 1187..1257
FT /note="5'-3' exoribonuclease 1 SH3-like"
FT /evidence="ECO:0000259|Pfam:PF18129"
FT REGION 382..412
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1266..1412
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1424..1486
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1327..1344
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1367..1381
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1486 AA; 165461 MW; CF4055A9C0688D71 CRC64;
MGVPKFFRWI SERYPAISQL IAENRIPEFD CLYLDMNGII HNCTHSDSDD ATTRVSEEEM
IIRIFNYIEH LFGKIKPKQM FFMAIDGVAP RAKMNQQRSR RFRTALDAEN ARKKAIEEGQ
QMPAEDPFDS NCITPGTDFM ARLSTQLKYF VNKKVSEDKD WQQCKVVLSG HDVPGEGEHK
IMEYIRNAKA QKDYNPNVRH CLYGLDADLI MLGLLSHDPH FCLLREEVTF GRQTRAKSKE
LEHQNFYLMH LCIVREYLEL EFQELKAENL MKFPFNLERI IDDFILMAFF IGNDFLPNLP
RLHINEGALA TMFSIYKRVL PQCDGYINEG GKVNIQRLGL LLAELSKEEF RFFEHEIEGE
KWFQAKLMSH VPETEGLTIA VDKKKGKHGR GNKSAAAAAA PTPTPPVKSA PGKLTVTTFQ
KELWKQKLRK FVNKPTDQPL DLGTDLKEDD RKFVQDLADS LRIDWRTDED DSGSRHLILT
FPAGRIAAKN ADDGDEEEEE EAQLAVSRVV RKYDTADVAD ITAEESQTAL EDLYMLKFQQ
WKDVYYKEKF HWSDTEITSQ VRTLCENYVQ GLQWVLFYYY RGIASWPWFY QYHYSPLISD
VIKGLGADLN FKLGQPFRPF EQLMGVLPDR SKKIVPPIYW GLMTEEASPI IDFYPRDFEL
DMNGKKMEWE AVVKIPFIDE KRLLDAMAPK NLLLSDDEKS RNEFGVPIEF SYLSDADFVY
PSSFTGIFPD IPHCHCIMNI FELPPTDGLD YLHDLTGGAM LNASALAGFP SLHTLPYTAA
LGYHGVNVFQ QESRNQSMVV NLTDSEARTN VEVAKKKLGK RCHIGYPFLQ EAKIVRVSDE
LFNYTLDDNG LIVQTPHAGR ELDNWNYKAR QIEEFYSKRL AILTGPIDSM VHIHMLRGLT
KTDEGATIKE YAEVLSMETT FATQVIVDDV INEDERFIER AAVPIAEEFP VGSHAFYLGD
FSYGRPLGVV GHNGNRMDVM VSVLQKKEPD FSHSIVQRAD RTNRYQPSFA VARALELHPL
SLSKITSSFF VFSVGNLKVN LGLNLKFEGK KQKVLGYSRK SASGWEFSGP AVQLIANYMV
KFPDFFAGLR YLPQNRDIKD TDIWPDQATA SQRVKEIGAW LKTQETSKLE RVPLDAEQLD
SVIVAQVAKA ADDAQVAAAP AETKSIKNVP RNAVLKPSDA EQRLGNQDFI LGDRIVYVQD
SGKVPIALRG TVVGISRTAT TKFLDVVFDS TFMSGTTLGD RCPPFRGQLV PASSVLNLSH
QQVVASSRAA SERKPVASIT TIPGQGGSRN GANAAGIRQY RDAPAPAPLR GGWRGALSGG
GGRGGRGNGS NGNNGSNGRG GNHAQEQSRP GPRPGTLLEP SNLVYRPAPG SSDQQPQQQA
GGRGGRGGRG GRGGRGQQHH AEATQAAAEA TAVFEEATQL SYNAVPAPAN LNPSHGGRGR
GRGRGRGGRG RGNGGGAGNE QKKNGDHSKS SGSSAAPAAG STAPAA
//
