ID S3CKS6_GLAL2 Unreviewed; 1693 AA.
AC S3CKS6;
DT 18-SEP-2013, integrated into UniProtKB/TrEMBL.
DT 18-SEP-2013, sequence version 1.
DT 27-MAR-2024, entry version 45.
DE RecName: Full=DNA-directed RNA polymerase subunit {ECO:0000256|RuleBase:RU004279};
DE EC=2.7.7.6 {ECO:0000256|RuleBase:RU004279};
GN ORFNames=GLAREA_01696 {ECO:0000313|EMBL:EPE25784.1};
OS Glarea lozoyensis (strain ATCC 20868 / MF5171).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC Helotiales; Helotiaceae; Glarea.
OX NCBI_TaxID=1116229 {ECO:0000313|EMBL:EPE25784.1, ECO:0000313|Proteomes:UP000016922};
RN [1] {ECO:0000313|EMBL:EPE25784.1, ECO:0000313|Proteomes:UP000016922}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 20868 / MF5171 {ECO:0000313|Proteomes:UP000016922};
RX PubMed=23688303; DOI=10.1186/1471-2164-14-339;
RA Chen L., Yue Q., Zhang X., Xiang M., Wang C., Li S., Che Y.,
RA Ortiz-Lopez F.J., Bills G.F., Liu X., An Z.;
RT "Genomics-driven discovery of the pneumocandin biosynthetic gene cluster in
RT the fungus Glarea lozoyensis.";
RL BMC Genomics 14:339-339(2013).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000256|RuleBase:RU004279}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550,
CC ECO:0000256|RuleBase:RU004279};
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000256|RuleBase:RU004279}.
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DR EMBL; KE145371; EPE25784.1; -; Genomic_DNA.
DR RefSeq; XP_008087103.1; XM_008088912.1.
DR STRING; 1116229.S3CKS6; -.
DR GeneID; 19460754; -.
DR KEGG; glz:GLAREA_01696; -.
DR eggNOG; KOG0262; Eukaryota.
DR HOGENOM; CLU_000487_2_3_1; -.
DR OMA; NREDYQQ; -.
DR OrthoDB; 169836at2759; -.
DR Proteomes; UP000016922; Unassembled WGS sequence.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR CDD; cd02735; RNAP_I_Rpa1_C; 1.
DR CDD; cd01435; RNAP_I_RPA1_N; 1.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.150.390; -; 1.
DR Gene3D; 1.10.357.120; -; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 3.30.70.2850; -; 1.
DR Gene3D; 3.30.1490.180; RNA polymerase ii; 1.
DR Gene3D; 4.10.860.120; RNA polymerase II, clamp domain; 1.
DR Gene3D; 1.10.274.100; RNA polymerase Rpb1, domain 3; 1.
DR InterPro; IPR047107; DNA-dir_RNA_pol1_lsu_C.
DR InterPro; IPR015699; DNA-dir_RNA_pol1_lsu_N.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; DNA-DIRECTED RNA POLYMERASE; 1.
DR PANTHER; PTHR19376:SF11; DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA1; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR SMART; SM00663; RPOLA_N; 1.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|RuleBase:RU004279};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004279}; Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000016922};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU004279};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004279}.
FT DOMAIN 354..681
FT /note="RNA polymerase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00663"
FT REGION 263..289
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1332..1387
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1399..1464
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1429..1464
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1693 AA; 187284 MW; 4D72CB6C79BE94BC CRC64;
MNISQPVSSA IDSVDFTFLS ASEILSLSVA RIENPETFDT LLHPVPQGLC STCKLKDKAC
PGHVGHIELP VPVYHPTFMT QMLRLLRAKC RYCHYLRLSR KEVNRYLCKL RLIQYGLLNA
AEEVDNIEVS NTEDADGSGG EDGQASSVEK LIRLRNAHVK RNIKVAKQNQ WDWQREKNEA
VAEARRVTIK EFLKTITTGK TCSNCKGISP TYRKDRFVKI FRKDLSPKEK AKMSLANIQA
KNAVTTLRER EKRKADGDFD EGIADIDLSS NESNEDEEGD GEDLDVDGDV VMGDTATKTA
RSSKSKVAAT PQIYCNPMEV KAELILLFEN EQEILSLVYN SRSTVKKAAV VSPDMFFMQT
LLVPPNKFRP EAKTGAGEIT ENPQNTLYKS ILRGCLLVSQ IYDEVNGLTE ETPYRIRDFG
DLQESWAGLQ DAVNSLIDRD RNPIQGAAGK KNEDGIKQKL EKKEGLFRKN MMGKRVNFAA
RSVISPDPNI ETNEIGVPPV FAKKLTYPEP VTSHNFKELQ QAVINGPDIW PGAQAIENEH
GQVISLTIKT PDERLALANQ LLAPSNNRAN GSRNKKVHRH LTNGDVVLMN RQPTLHKPSM
MGHRARVLTG EKTIRMHYAN CNTYNADFDG DEMNMHFPQN EVARAEVLQI ADTDHQYLAG
TFGKPLRGLI QDHISVSVWM CSRDTFFDRA TYQQLIYNSL RPESGHIVGD RIETVTPAII
KPKPLWTGKQ VITTILRNIK PVDCAPLGLN SKPSVPGDRW GSDSNEDRVL FKGGEFITGI
LDKAQIGPSG GGFIHAIHEV YGPTAAGKTL SILGRLLTKF LHMRAFTCGM DDLRLTPRGE
QARRDTLKEA HRLGLEVASK YVSLQDQKPT DTDAELMERM EDVLRDDSKQ GGLDVMMNAR
SGNLSSQITK ACLPDGLVKQ FPKNQMQNMT VSGAKGSAVN ANLISCNLGQ QVLEGRRVPV
MVSGKSLPCF RPYETNIRAG GYIVNRFLSG IRPQEYYFHA MAGREGLIDT AVKTSRSGYL
QRCLIKGMEG LKVEYDTSVR DSDGSMVQFL YGEDGLDVTK QVHLQDFKFL LENITSELSQ
LNYGDPNYEQ IFSEKEIILK KMKKALKYSK ANDPNGPDPV LSSFNPGKYG YATSEKFYDA
LTNYLKANPD GMVKDKEHPD GVGVRKALEP VLMAKYLKSV VDPGEAVGIV AGQSVGEPST
QMTLNTFHLA GHSTKNVTLG IPRLREIVMT ASNHISTPSM TLRVNDELSA EDGEKFAKGI
SVLSLGEVLD EATVKERVGR GVAFSEARIY EVQVKLFPSK EYIDTYAITV ADVLHTLEKK
FAPKLQALTR RALGRKKEEK SSKSTKGGVP EIGKSVGAIE DPAAATRRGS EDPQAEDEDD
DIEEDDAKQV NANALVSYAE NDEEDDQVQA QMQKDSEQDD ADVPDMEDEG IGGSPREPDS
DGEGQQKVDR QEQRATEASA EREARVMKNC SDVVRFRFDE EAGEWCDITL EYAADVPKIL
MMTVVEDALR QCLIQQIPGL GACNYIPNQD SSKYGNKIHL PVVQTVGVNL KAMQTYPHII
NPNTISTNDI AAMLANYGVE ACRATIIQEL AGVFGGHAIS VDNRHLNLIA DFMTRGGGFS
PFNRNGLRGS VSPFMKMSFE TTVGFLADAV ADGDFDDLSN PSSRIVVGRM SKVGTGAFDV
LTKVPTVRSD EES
//
