ID S3DLJ2_GLAL2 Unreviewed; 363 AA.
AC S3DLJ2;
DT 18-SEP-2013, integrated into UniProtKB/TrEMBL.
DT 18-SEP-2013, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE RecName: Full=4-dimethylallyltryptophan N-methyltransferase {ECO:0000256|ARBA:ARBA00039094};
DE EC=2.1.1.261 {ECO:0000256|ARBA:ARBA00039094};
GN ORFNames=GLAREA_05928 {ECO:0000313|EMBL:EPE32916.1};
OS Glarea lozoyensis (strain ATCC 20868 / MF5171).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC Helotiales; Helotiaceae; Glarea.
OX NCBI_TaxID=1116229 {ECO:0000313|EMBL:EPE32916.1, ECO:0000313|Proteomes:UP000016922};
RN [1] {ECO:0000313|EMBL:EPE32916.1, ECO:0000313|Proteomes:UP000016922}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 20868 / MF5171 {ECO:0000313|Proteomes:UP000016922};
RX PubMed=23688303; DOI=10.1186/1471-2164-14-339;
RA Chen L., Yue Q., Zhang X., Xiang M., Wang C., Li S., Che Y.,
RA Ortiz-Lopez F.J., Bills G.F., Liu X., An Z.;
RT "Genomics-driven discovery of the pneumocandin biosynthetic gene cluster in
RT the fungus Glarea lozoyensis.";
RL BMC Genomics 14:339-339(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-(3-methylbut-2-enyl)-L-tryptophan + S-adenosyl-L-methionine
CC = 4-(3-methylbut-2-enyl)-L-abrine + H(+) + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:34435, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856,
CC ChEBI:CHEBI:58209, ChEBI:CHEBI:59789, ChEBI:CHEBI:67248;
CC EC=2.1.1.261; Evidence={ECO:0000256|ARBA:ARBA00036016};
CC -!- PATHWAY: Alkaloid biosynthesis; ergot alkaloid biosynthesis.
CC {ECO:0000256|ARBA:ARBA00005107}.
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC {ECO:0000256|ARBA:ARBA00008361}.
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DR EMBL; KE145358; EPE32916.1; -; Genomic_DNA.
DR RefSeq; XP_008079533.1; XM_008081342.1.
DR AlphaFoldDB; S3DLJ2; -.
DR GeneID; 19464982; -.
DR KEGG; glz:GLAREA_05928; -.
DR eggNOG; ENOG502QS9T; Eukaryota.
DR HOGENOM; CLU_049766_0_2_1; -.
DR OMA; FGCSYKY; -.
DR OrthoDB; 45787at2759; -.
DR Proteomes; UP000016922; Unassembled WGS sequence.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0009820; P:alkaloid metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR019257; MeTrfase_dom.
DR InterPro; IPR017804; MeTrfase_EgtD-like.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR017805; SAM_MeTrfase_EasF-type_put.
DR NCBIfam; TIGR03439; methyl_EasF; 1.
DR PANTHER; PTHR43397; ERGOTHIONEINE BIOSYNTHESIS PROTEIN 1; 1.
DR PANTHER; PTHR43397:SF1; ERGOTHIONEINE BIOSYNTHESIS PROTEIN 1; 1.
DR Pfam; PF10017; Methyltransf_33; 1.
DR PIRSF; PIRSF018005; UCP018005; 1.
PE 3: Inferred from homology;
KW Alkaloid metabolism {ECO:0000256|ARBA:ARBA00022589};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000016922};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 26..352
FT /note="Histidine-specific methyltransferase SAM-dependent"
FT /evidence="ECO:0000259|Pfam:PF10017"
SQ SEQUENCE 363 AA; 40951 MW; B221D4F285A0584D CRC64;
MTTHHSTRKR PLILDIRQLD FEDSITKQVI DGLSNKPRTL PALLFYSTEG LKHWNVHSQQ
AEFYPKHEEL RVIKDRAHDI ATSIEPNSVI VDLGSASLDK VIPLLEALES QKKNIRYYAL
DLSAEELAST LQAIPTERFQ HVHFAALHGT FEDGLLWLKN TPEICNLPHC LLLLGLTIGN
FSRENAAAFL RSIAAHDRPQ SRKSGETDSR TSIILTIDSC KTPTKILRAY NAEGVVPFAL
TALKYANVLL GKEAEQSDQN FPFVFESEEW HFLSQWNHVL GRHEASLVPK NENIKLGAPL
DAIVVGKEEA VRFGCSYKYD KDQRRELFAA AGVEEAAVWS HDECDVAFYK LQTCIDSIAY
QEV
//
