ID S5LZ88_9MOLU Unreviewed; 478 AA.
AC S5LZ88;
DT 16-OCT-2013, integrated into UniProtKB/TrEMBL.
DT 16-OCT-2013, sequence version 1.
DT 27-MAR-2024, entry version 46.
DE RecName: Full=Pyruvate kinase {ECO:0000256|ARBA:ARBA00012142, ECO:0000256|RuleBase:RU000504};
DE EC=2.7.1.40 {ECO:0000256|ARBA:ARBA00012142, ECO:0000256|RuleBase:RU000504};
GN Name=pyk {ECO:0000313|EMBL:AGR41891.1};
GN ORFNames=SDIMI_v3c01870 {ECO:0000313|EMBL:AGR41891.1};
OS Spiroplasma diminutum CUAS-1.
OC Bacteria; Mycoplasmatota; Mollicutes; Entomoplasmatales; Spiroplasmataceae;
OC Spiroplasma.
OX NCBI_TaxID=1276221 {ECO:0000313|EMBL:AGR41891.1, ECO:0000313|Proteomes:UP000014983};
RN [1] {ECO:0000313|EMBL:AGR41891.1, ECO:0000313|Proteomes:UP000014983}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CUAS-1 {ECO:0000313|EMBL:AGR41891.1};
RX PubMed=23873917; DOI=10.1093/gbe/evt108;
RA Lo W.S., Ku C., Chen L.L., Chang T.H., Kuo C.H.;
RT "Comparison of metabolic capacities and inference of gene content evolution
RT in mosquito-associated Spiroplasma diminutum and S. taiwanense.";
RL Genome Biol. Evol. 5:1512-1523(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + pyruvate = ADP + H(+) + phosphoenolpyruvate;
CC Xref=Rhea:RHEA:18157, ChEBI:CHEBI:15361, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:58702, ChEBI:CHEBI:456216;
CC EC=2.7.1.40; Evidence={ECO:0000256|RuleBase:RU000504};
CC -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC glyceraldehyde 3-phosphate: step 5/5. {ECO:0000256|ARBA:ARBA00004997,
CC ECO:0000256|RuleBase:RU000504}.
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|ARBA:ARBA00011881}.
CC -!- SIMILARITY: Belongs to the pyruvate kinase family.
CC {ECO:0000256|ARBA:ARBA00008663, ECO:0000256|RuleBase:RU000504}.
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DR EMBL; CP005076; AGR41891.1; -; Genomic_DNA.
DR RefSeq; WP_020836124.1; NC_021833.1.
DR AlphaFoldDB; S5LZ88; -.
DR STRING; 1276221.SDIMI_v3c01870; -.
DR KEGG; sdi:SDIMI_v3c01870; -.
DR PATRIC; fig|1276221.3.peg.184; -.
DR eggNOG; COG0469; Bacteria.
DR HOGENOM; CLU_015439_0_2_14; -.
DR InParanoid; S5LZ88; -.
DR OrthoDB; 9812123at2; -.
DR UniPathway; UPA00109; UER00188.
DR Proteomes; UP000014983; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0030955; F:potassium ion binding; IEA:InterPro.
DR GO; GO:0004743; F:pyruvate kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR Gene3D; 3.20.20.60; Phosphoenolpyruvate-binding domains; 1.
DR Gene3D; 2.40.33.10; PK beta-barrel domain-like; 1.
DR Gene3D; 3.40.1380.20; Pyruvate kinase, C-terminal domain; 1.
DR InterPro; IPR001697; Pyr_Knase.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR InterPro; IPR040442; Pyrv_Kinase-like_dom_sf.
DR InterPro; IPR011037; Pyrv_Knase-like_insert_dom_sf.
DR InterPro; IPR015793; Pyrv_Knase_brl.
DR InterPro; IPR036918; Pyrv_Knase_C_sf.
DR InterPro; IPR015806; Pyrv_Knase_insert_dom_sf.
DR NCBIfam; TIGR01064; pyruv_kin; 1.
DR PANTHER; PTHR11817; PYRUVATE KINASE; 1.
DR PANTHER; PTHR11817:SF132; PYRUVATE KINASE 1; 1.
DR Pfam; PF00224; PK; 1.
DR PRINTS; PR01050; PYRUVTKNASE.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR SUPFAM; SSF50800; PK beta-barrel domain-like; 1.
DR SUPFAM; SSF52935; PK C-terminal domain-like; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Glycolysis {ECO:0000256|ARBA:ARBA00023152, ECO:0000256|RuleBase:RU000504};
KW Kinase {ECO:0000256|ARBA:ARBA00022777, ECO:0000256|RuleBase:RU000504};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|RuleBase:RU000504};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Pyruvate {ECO:0000256|ARBA:ARBA00023317, ECO:0000313|EMBL:AGR41891.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000014983};
KW Transferase {ECO:0000256|RuleBase:RU000504}.
FT DOMAIN 10..336
FT /note="Pyruvate kinase barrel"
FT /evidence="ECO:0000259|Pfam:PF00224"
SQ SEQUENCE 478 AA; 53829 MW; AD12DC0A850526B5 CRC64;
MNKFNLTDKM KRTKVITTIG PSVHSKEAIK ELFDKGMTTI RLNFSHADFQ EHGERFEWVK
ELRKEINKPI SILLDTKGPE IRIGKMKDGK QEVKAGTEIT VYTNPEDFSS RECSATEMQM
SYDMSQDVKP GDVVLVDDGK LTMHVISVEK FKVMCTAFNT HLVKTNKRVN LPGVEFTLPF
LAEKDYNDIH FGIESNIDYI AASFVNSADN VNEIRAILKA KNAEHIQIIS KIESQVGCDN
IDSIIDASDG IMVARGDLGL EIPYYDVPYW EKQIIRKCRE KGKLVIVATQ MLESMTDNPQ
PTRAEVTDVY YATELGADAT MLSGESANGD FPFITVETMS TINKRAEVEF YEKNYYTKQL
EKARKSSSGK RAEIANQLAN TTMGGNYEYA IVVSRTGELL RTISKFRPNV TILGVCDNEK
LWTGFGAMHS IFMSKVEDLD AFMNDDKAIA EIAKSWGAKS GERILFVRNE NIKEITVA
//
