ID S5Y1S8_PARAH Unreviewed; 975 AA.
AC S5Y1S8;
DT 16-OCT-2013, integrated into UniProtKB/TrEMBL.
DT 16-OCT-2013, sequence version 1.
DT 27-MAR-2024, entry version 50.
DE SubName: Full=Sarcosine oxidase, subunit alpha {ECO:0000313|EMBL:AGT11427.1};
DE EC=1.5.3.1 {ECO:0000313|EMBL:AGT11427.1};
GN ORFNames=JCM7686_pAMI6p097 {ECO:0000313|EMBL:AGT11427.1};
OS Paracoccus aminophilus JCM 7686.
OG Plasmid pAMI6 {ECO:0000313|EMBL:AGT11427.1,
OG ECO:0000313|Proteomes:UP000015480}.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodobacterales;
OC Paracoccaceae; Paracoccus.
OX NCBI_TaxID=1367847 {ECO:0000313|EMBL:AGT11427.1, ECO:0000313|Proteomes:UP000015480};
RN [1] {ECO:0000313|EMBL:AGT11427.1, ECO:0000313|Proteomes:UP000015480}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 7686 {ECO:0000313|EMBL:AGT11427.1};
RC PLASMID=Plasmid pAMI6 {ECO:0000313|Proteomes:UP000015480};
RX PubMed=24517536; DOI=10.1186/1471-2164-15-124;
RA Dziewit L., Czarnecki J., Wibberg D., Radlinska M., Mrozek P., Szymczak M.,
RA Schluter A., Puhler A., Bartosik D.;
RT "Architecture and functions of a multipartite genome of the methylotrophic
RT bacterium Paracoccus aminophilus JCM 7686, containing primary and secondary
RT chromids.";
RL BMC Genomics 15:124-124(2014).
CC -!- SIMILARITY: Belongs to the GcvT family.
CC {ECO:0000256|ARBA:ARBA00008609}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP006654; AGT11427.1; -; Genomic_DNA.
DR RefSeq; WP_020953198.1; NC_022044.1.
DR AlphaFoldDB; S5Y1S8; -.
DR KEGG; pami:JCM7686_pAMI6p097; -.
DR PATRIC; fig|1367847.3.peg.4402; -.
DR HOGENOM; CLU_011963_0_0_5; -.
DR OrthoDB; 5287468at2; -.
DR Proteomes; UP000015480; Plasmid pAMI6.
DR GO; GO:0008115; F:sarcosine oxidase activity; IEA:UniProtKB-EC.
DR GO; GO:0046653; P:tetrahydrofolate metabolic process; IEA:InterPro.
DR Gene3D; 3.10.20.440; 2Fe-2S iron-sulphur cluster binding domain, sarcosine oxidase, alpha subunit, N-terminal domain; 1.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 1.
DR InterPro; IPR042204; 2Fe-2S-bd_N.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR028896; GCST/YgfZ/DmdA.
DR InterPro; IPR013977; GCV_T_C.
DR InterPro; IPR006222; GCV_T_N.
DR InterPro; IPR029043; GcvT/YgfZ_C.
DR InterPro; IPR006277; Sarcosine_oxidase_asu.
DR InterPro; IPR041117; SoxA_A3.
DR InterPro; IPR027266; TrmE/GcvT_dom1.
DR NCBIfam; TIGR01372; soxA; 1.
DR PANTHER; PTHR43757; AMINOMETHYLTRANSFERASE; 1.
DR PANTHER; PTHR43757:SF2; AMINOMETHYLTRANSFERASE, MITOCHONDRIAL; 1.
DR Pfam; PF12831; FAD_oxidored; 1.
DR Pfam; PF13510; Fer2_4; 1.
DR Pfam; PF01571; GCV_T; 1.
DR Pfam; PF08669; GCV_T_C; 1.
DR Pfam; PF17806; SO_alpha_A3; 1.
DR PIRSF; PIRSF037980; SoxA; 1.
DR PRINTS; PR00368; FADPNR.
DR PRINTS; PR00469; PNDRDTASEII.
DR SUPFAM; SSF101790; Aminomethyltransferase beta-barrel domain; 1.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
DR SUPFAM; SSF103025; Folate-binding domain; 1.
PE 3: Inferred from homology;
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000313|EMBL:AGT11427.1}; Plasmid {ECO:0000313|EMBL:AGT11427.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000015480}.
FT DOMAIN 491..576
FT /note="SoxA A3"
FT /evidence="ECO:0000259|Pfam:PF17806"
FT DOMAIN 591..855
FT /note="Aminomethyltransferase folate-binding"
FT /evidence="ECO:0000259|Pfam:PF01571"
FT DOMAIN 880..967
FT /note="Glycine cleavage T-protein C-terminal barrel"
FT /evidence="ECO:0000259|Pfam:PF08669"
SQ SEQUENCE 975 AA; 105016 MW; 1BDF9F2C85C71922 CRC64;
MTRLSGGLVD RSRSLTFSFD GKSYSGFAGD TLASALLAND VRLMGRGFKY HRPRGVFSAG
SEEPNALVEL RGGAWQEPNT RATVAELFDG LEAKSQNRVG SLSFDLLAVN DLLSPFFAAG
FYYKTFMWPR AFWEKIYEPM IRRAAGLGSL SMQADPDSYD SGFLHCDLLV IGGGAAGLAA
ALTAARTGAS VILADEDFRL GGRLLAESAP LDRPASEWIA ELEKEFASLP NLRVMRRTTV
WGAFDHGIYG AVERVSDHLV TPGKRVRQTL WRITSKRAIL AAGATERHIP FANNDRPGVM
LAGAMRSYAN RWAVSPAQKV AIFTNNDDGH RTALDLAAKG VDITAVIDSR SGAKSQGGYR
TIAGGQVSDS KGRLGLTKVE VTANGKTEWV DCGALGVAGG WNPNLHIASH HRERPVWNDA
LQTFLAVEGP VGLRIVGAAA GKGSTAEALA SGAEAAQAAL ADLGITAHLP ELPKGEDHAL
NLQPLWHVPG KGRAWVDFQN DVTVKDVKLA WQENLRPVEH LKRWTTLGMA TDQGKTSNVT
AIAVMSELTG QSINQTGTTI FRPPYTPVSM SVLGGGDTGS HFRPRRLTPT HPWAAAQGAV
FVEVGQWMRA QYFPRPGETT WRETVDREVL ATRKGVGICD VTTLGKIDVQ GADAGEFLDR
VYANAMKSLK VGMVRYGLML REDGFAWDDG TCARLSETDY VVTTTTANAG TIYKHMEFCR
QCLWPELDVQ LISTTDAWAQ MSVAGPNARK LLERVVDGFD LSNEAFPYMA CGDLTVCGGL
RARLFRISFS GELAYEIAVP ARYGNALMAR MIELGQDLGA TPYGTEALGV LRIEKGHAAG
NELNGQTTAQ MLGMGKFVST KKDSIGAVLS RREGMVADPR VLVGFQPVDA SDPVVAGSHL
FEEGAEQNTW TDEGWITSAC YSPHLGSAIG LGYLANGADR KGEVIVAANP LQGQSVRLRV
VSAHFVDPEG GRVRD
//
