ID S6HG39_9GAMM Unreviewed; 936 AA.
AC S6HG39;
DT 16-OCT-2013, integrated into UniProtKB/TrEMBL.
DT 16-OCT-2013, sequence version 1.
DT 27-MAR-2024, entry version 44.
DE SubName: Full=Aconitase B {ECO:0000313|EMBL:EPJ43917.1};
GN ORFNames=OFPII_38510 {ECO:0000313|EMBL:EPJ43917.1};
OS Osedax symbiont Rs1.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Oceanospirillales;
OC Oceanospirillaceae.
OX NCBI_TaxID=1330036 {ECO:0000313|EMBL:EPJ43917.1, ECO:0000313|Proteomes:UP000014822};
RN [1] {ECO:0000313|EMBL:EPJ43917.1, ECO:0000313|Proteomes:UP000014822}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Rs1 {ECO:0000313|Proteomes:UP000014822};
RA Yi H., Goffredi S.;
RT "Comparative genomics of two novel heterotrophic symbionts of deep-sea
RT Osedax worms: functional variation and environmental selection.";
RL Submitted (MAY-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = 2-methyl-cis-
CC aconitate + H2O; Xref=Rhea:RHEA:17941, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:57429, ChEBI:CHEBI:57872; EC=4.2.1.99;
CC Evidence={ECO:0000256|ARBA:ARBA00000118};
CC -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family.
CC {ECO:0000256|ARBA:ARBA00007185}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EPJ43917.1}.
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DR EMBL; ASZJ01000182; EPJ43917.1; -; Genomic_DNA.
DR AlphaFoldDB; S6HG39; -.
DR PATRIC; fig|1330036.3.peg.3689; -.
DR Proteomes; UP000014822; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003994; F:aconitate hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR Gene3D; 3.40.1060.10; Aconitase, Domain 2; 1.
DR Gene3D; 3.30.499.10; Aconitase, domain 3; 2.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR Gene3D; 1.25.40.310; Aconitate B, HEAT-like domain; 1.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR InterPro; IPR015933; Aconitase_B_HEAT-like_dom.
DR InterPro; IPR036288; Aconitase_B_HEAT-like_dom_sf.
DR InterPro; IPR015929; Aconitase_B_swivel.
DR InterPro; IPR015932; Aconitase_dom2.
DR PANTHER; PTHR43160; ACONITATE HYDRATASE B; 1.
DR PANTHER; PTHR43160:SF4; ACONITATE HYDRATASE B; 1.
DR Pfam; PF00330; Aconitase; 1.
DR Pfam; PF06434; Aconitase_2_N; 1.
DR Pfam; PF11791; Aconitase_B_N; 1.
DR SUPFAM; SSF74778; Aconitase B, N-terminal domain; 1.
DR SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000014822};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884}.
FT DOMAIN 7..163
FT /note="Aconitase B HEAT-like"
FT /evidence="ECO:0000259|Pfam:PF11791"
FT DOMAIN 177..406
FT /note="Aconitase B swivel"
FT /evidence="ECO:0000259|Pfam:PF06434"
FT DOMAIN 410..894
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
SQ SEQUENCE 936 AA; 101763 MW; 5023CE74B80EFCD2 CRC64;
MNLYLDYIEE IKSRKTDLGL APLPIDSSEL LTEIITQIKD LGNEHREDSL NFFIYNTLPG
TTSAAGVKAK FLKEIILAES VVAEITPSFA FELLSHMKGG PSVEVLLDLS LGDDAAIATS
AAEVLKTQVF LYDADTERLA TAFKNGNSIA KDVLESYARA DFFTKLPEID EEIDVVTYIA
AEGDISTDLL SPGNQAHSRS DRELHGKCMI SPEAQAKIEE FKTLYPGKKV MLIAEKGTMG
VGSSRMSGVN NVALWAGEQA SPYIPFVNFA PVVAGTNGIS PIFLTTVGVT GGIGLDLKNW
VKKLDANGKT VLNDNGDPVL VQSYSVETGT VLTINTKQKK LYKGDQELVD ISSAFTPQSV
EFMKAGGSYA IVFGKKLQNF ASQTLSIALK PVFAPSQEIS HAGQGLTAVE KIFNKNAVGV
TEGAVLHAGS DVRVTVNIVG SQDTTGLMTS QELESMAATT ISPIVDGAYQ SGCHTASVWD
KKAQANIPKL MSFMHNFGLI TARDPEGVYL PMTDVIHKVL NDITIDEWAI IIGGDSHTRM
SKGVAFGADS GTVALALATG EATMPIPESV KVTFKGKMKG HIDFRDVVHA TQSQMLKQFG
DNIFQGRIIE VHIGTLLADQ AFTFTDWTAE MKAKGSICIS QDDKLIESLE IAKSRIQIMI
NKGMENDARV LHGLIAKADQ RISEITSGEI PALKPDNNAK YYSELVVDLD LIDEPMIADP
DVNNEDVSKR YTHDVIRQLS YYDGSRDVDL GFVGSCMVHK GDLQIISQMF RNIEAKNGSI
EFKAPLIVAA PTYNIIDELK AEGDWDILQK YSGFEFDDSN PKESARTKYE NMMYLERPGC
NLCMGNQEKA EKGDTVLATS TRLFQGRVVE DSERKKGESL LASTPVVVLS AILGRTPSMD
EYKKAVDGIN LTDFAPPLEA MTTTPTAVPV KIMNPM
//
