ID S7NGC8_MYOBR Unreviewed; 654 AA.
AC S7NGC8;
DT 16-OCT-2013, integrated into UniProtKB/TrEMBL.
DT 16-OCT-2013, sequence version 1.
DT 24-JAN-2024, entry version 29.
DE RecName: Full=Beta-galactosidase {ECO:0000256|RuleBase:RU000675};
DE EC=3.2.1.23 {ECO:0000256|RuleBase:RU000675};
GN ORFNames=D623_10003679 {ECO:0000313|EMBL:EPQ16514.1};
OS Myotis brandtii (Brandt's bat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Chiroptera; Microchiroptera; Vespertilionidae;
OC Myotis.
OX NCBI_TaxID=109478 {ECO:0000313|EMBL:EPQ16514.1, ECO:0000313|Proteomes:UP000052978};
RN [1] {ECO:0000313|Proteomes:UP000052978}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=23962925; DOI=10.1038/ncomms3212;
RA Seim I., Fang X., Xiong Z., Lobanov A.V., Huang Z., Ma S., Feng Y.,
RA Turanov A.A., Zhu Y., Lenz T.L., Gerashchenko M.V., Fan D., Hee Yim S.,
RA Yao X., Jordan D., Xiong Y., Ma Y., Lyapunov A.N., Chen G., Kulakova O.I.,
RA Sun Y., Lee S.G., Bronson R.T., Moskalev A.A., Sunyaev S.R., Zhang G.,
RA Krogh A., Wang J., Gladyshev V.N.;
RT "Genome analysis reveals insights into physiology and longevity of the
RT Brandt's bat Myotis brandtii.";
RL Nat. Commun. 4:2212-2212(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|RuleBase:RU000675};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC {ECO:0000256|ARBA:ARBA00009809, ECO:0000256|RuleBase:RU003679}.
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DR EMBL; KE164266; EPQ16514.1; -; Genomic_DNA.
DR AlphaFoldDB; S7NGC8; -.
DR eggNOG; KOG0496; Eukaryota.
DR Proteomes; UP000052978; Unassembled WGS sequence.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 2.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR026283; B-gal_1-like.
DR InterPro; IPR048912; BetaGal1-like_ABD1.
DR InterPro; IPR048913; BetaGal_gal-bd.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR InterPro; IPR019801; Glyco_hydro_35_CS.
DR InterPro; IPR001944; Glycoside_Hdrlase_35.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR23421; BETA-GALACTOSIDASE RELATED; 1.
DR PANTHER; PTHR23421:SF53; BETA-GALACTOSIDASE-1-LIKE PROTEIN 2; 1.
DR Pfam; PF21317; BetaGal_ABD_1; 1.
DR Pfam; PF21467; BetaGal_gal-bd; 1.
DR Pfam; PF01301; Glyco_hydro_35; 1.
DR PIRSF; PIRSF006336; B-gal; 1.
DR PRINTS; PR00742; GLHYDRLASE35.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU000675};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU000675};
KW Reference proteome {ECO:0000313|Proteomes:UP000052978}.
FT DOMAIN 69..383
FT /note="Glycoside hydrolase 35 catalytic"
FT /evidence="ECO:0000259|Pfam:PF01301"
FT DOMAIN 443..542
FT /note="Beta-galactosidase 1-like first all-beta"
FT /evidence="ECO:0000259|Pfam:PF21317"
FT DOMAIN 566..625
FT /note="Beta-galactosidase galactose-binding"
FT /evidence="ECO:0000259|Pfam:PF21467"
FT ACT_SITE 217
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
FT ACT_SITE 293
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
SQ SEQUENCE 654 AA; 74098 MW; 72775B4373E46834 CRC64;
MTTWSAPRRP CRALGLLLLV VLGFLVLRRY LHLEPGSCGQ EVAQRLHWPR LLSPWLRHRQ
SGLHTKGQNF MLEDSTFWIF GGSVHYFRVP RAYWRDRLLK MKACGLNTLT TYVPWNLHEP
QRGTFDFSGN LDLEAFVRMA AEVGLWVILR PGPYICSEID LGGLPSWLLR DSGMRLRTTY
KGFTEAVDLY FDHLMSRVVP LQYKHGGPII AVQVENEYGS YNKDPAYLPY IKKALEDRGI
VELLLTSDNK DGLSNGVIDG VLATINLQSQ HDLQLLTIFL LSVQGVQPKM VMEYWTGWFD
SWGGPHNILD ASEVLKTVSA IIDAGSSINL YMFHGGTNFG FINGAMHFHD YKSDVTSYGK
YYDAVLTEAG DYTAKYVKLR EFFGSISGSP LPPPPELLPK TAYESLRPAL YLSLWDALQY
MESPIISEKP VNMENLPVNN GNGQSFGYTL YETTIGSPGV LRGLVRDRGQ VFVNTVFIGF
LDYERKDIVI PLIQGYTVLR ILVENCGRVN YGHNIDEQRK GLIGNIYLNN SPLKNFRIYS
LDMKKSFFQR FSSSAWNLVP ETPVFPAFFL GALPVALSPL DTFMKLEGWE KGVVFINGQN
LGRYWNIGPQ ETLYLPGAWL DQGINQVIVF EERAAGPVIQ FTETPHLGRN QYIH
//
