ID SART3_DANRE Reviewed; 951 AA.
AC B3DJT0;
DT 07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT 22-JUL-2008, sequence version 1.
DT 27-MAR-2024, entry version 87.
DE RecName: Full=Squamous cell carcinoma antigen recognized by T-cells 3 {ECO:0000305};
DE Short=SART-3;
GN Name=sart3 {ECO:0000312|ZFIN:ZDB-GENE-040724-10}; Synonyms=egy;
GN ORFNames=si:ch211-191d15.4, wu:fc51h03;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955 {ECO:0000312|EMBL:AAI63594.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=17416673; DOI=10.1073/pnas.0701919104;
RA Trede N.S., Medenbach J., Damianov A., Hung L.H., Weber G.J., Paw B.H.,
RA Zhou Y., Hersey C., Zapata A., Keefe M., Barut B.A., Stuart A.B., Katz T.,
RA Amemiya C.T., Zon L.I., Bindereif A.;
RT "Network of coregulated spliceosome components revealed by zebrafish mutant
RT in recycling factor p110.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:6608-6613(2007).
CC -!- FUNCTION: U6 snRNP-binding protein that functions as a recycling factor
CC of the splicing machinery. Promotes the initial reassembly of U4 and U6
CC snRNPs following their ejection from the spliceosome during its
CC maturation (PubMed:17416673). May also function as a substrate
CC targeting factor for deubiquitinases and mediate the deubiquitination
CC of components of the spliceosome and histones (By similarity).
CC {ECO:0000250|UniProtKB:Q15020, ECO:0000269|PubMed:17416673}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleoplasm
CC {ECO:0000250|UniProtKB:Q15020}. Nucleus, Cajal body
CC {ECO:0000250|UniProtKB:Q15020}. Nucleus speckle
CC {ECO:0000250|UniProtKB:Q15020}. Cytoplasm
CC {ECO:0000250|UniProtKB:Q15020}.
CC -!- DISRUPTION PHENOTYPE: The egy mutant embryos that lack detectable sart3
CC expression display microcephaly, microphthalmia and die by 7 to 8 dpf.
CC Pharyngeal arch formation is defective resulting in a thymus devoid of
CC lymphocytes and exocrine pancreas development is also impaired.
CC {ECO:0000269|PubMed:17416673}.
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DR EMBL; AL845326; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC163594; AAI63594.1; -; mRNA.
DR RefSeq; XP_005165211.1; XM_005165154.3.
DR RefSeq; XP_017211593.1; XM_017356104.1.
DR RefSeq; XP_017211594.1; XM_017356105.1.
DR AlphaFoldDB; B3DJT0; -.
DR SMR; B3DJT0; -.
DR STRING; 7955.ENSDARP00000004923; -.
DR PaxDb; 7955-ENSDARP00000004923; -.
DR PeptideAtlas; B3DJT0; -.
DR GeneID; 558581; -.
DR AGR; ZFIN:ZDB-GENE-040724-10; -.
DR CTD; 9733; -.
DR ZFIN; ZDB-GENE-040724-10; sart3.
DR eggNOG; KOG0128; Eukaryota.
DR HOGENOM; CLU_007172_0_0_1; -.
DR InParanoid; B3DJT0; -.
DR OMA; LWARYIL; -.
DR OrthoDB; 2879516at2759; -.
DR PhylomeDB; B3DJT0; -.
DR TreeFam; TF317554; -.
DR ChiTaRS; sart3; zebrafish.
DR PRO; PR:B3DJT0; -.
DR Proteomes; UP000000437; Chromosome 5.
DR Bgee; ENSDARG00000008032; Expressed in presomitic mesoderm and 28 other cell types or tissues.
DR ExpressionAtlas; B3DJT0; baseline and differential.
DR GO; GO:0061574; C:ASAP complex; IBA:GO_Central.
DR GO; GO:0015030; C:Cajal body; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
DR GO; GO:0005654; C:nucleoplasm; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0042393; F:histone binding; ISS:UniProtKB.
DR GO; GO:0017070; F:U6 snRNA binding; ISS:UniProtKB.
DR GO; GO:0031017; P:exocrine pancreas development; IMP:ZFIN.
DR GO; GO:0061484; P:hematopoietic stem cell homeostasis; IMP:ZFIN.
DR GO; GO:0030098; P:lymphocyte differentiation; IMP:ZFIN.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR GO; GO:0006334; P:nucleosome assembly; ISS:UniProtKB.
DR GO; GO:1902033; P:regulation of hematopoietic stem cell proliferation; IMP:ZFIN.
DR GO; GO:1902253; P:regulation of intrinsic apoptotic signaling pathway by p53 class mediator; IMP:ZFIN.
DR GO; GO:0000245; P:spliceosomal complex assembly; IMP:ZFIN.
DR GO; GO:0000387; P:spliceosomal snRNP assembly; IMP:UniProtKB.
DR GO; GO:0048538; P:thymus development; IMP:ZFIN.
DR GO; GO:0140673; P:transcription elongation-coupled chromatin remodeling; ISS:UniProtKB.
DR CDD; cd12391; RRM1_SART3; 1.
DR CDD; cd12392; RRM2_SART3; 1.
DR Gene3D; 3.30.70.330; -; 2.
DR Gene3D; 1.25.40.10; Tetratricopeptide repeat domain; 2.
DR InterPro; IPR003107; HAT.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR InterPro; IPR034217; SART3_RRM1.
DR InterPro; IPR034218; SART3_RRM2.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR PANTHER; PTHR17204:SF25; EMBRYO DEFECTIVE 140; 1.
DR PANTHER; PTHR17204; PRE-MRNA PROCESSING PROTEIN PRP39-RELATED; 1.
DR Pfam; PF16605; LSM_int_assoc; 1.
DR Pfam; PF00076; RRM_1; 2.
DR SMART; SM00386; HAT; 7.
DR SMART; SM00360; RRM; 2.
DR SUPFAM; SSF54928; RNA-binding domain, RBD; 2.
DR SUPFAM; SSF48452; TPR-like; 1.
DR PROSITE; PS50102; RRM; 2.
PE 2: Evidence at transcript level;
KW Coiled coil; Cytoplasm; mRNA processing; mRNA splicing; Nucleus;
KW Reference proteome; Repeat; RNA-binding.
FT CHAIN 1..951
FT /note="Squamous cell carcinoma antigen recognized by T-
FT cells 3"
FT /id="PRO_0000431579"
FT REPEAT 88..120
FT /note="HAT 1"
FT /evidence="ECO:0000255"
FT REPEAT 126..157
FT /note="HAT 2"
FT /evidence="ECO:0000255"
FT REPEAT 163..199
FT /note="HAT 3"
FT /evidence="ECO:0000255"
FT REPEAT 222..255
FT /note="HAT 4"
FT /evidence="ECO:0000255"
FT REPEAT 304..336
FT /note="HAT 5"
FT /evidence="ECO:0000255"
FT REPEAT 339..371
FT /note="HAT 6"
FT /evidence="ECO:0000255"
FT REPEAT 374..410
FT /note="HAT 7"
FT /evidence="ECO:0000255"
FT REPEAT 467..500
FT /note="HAT 8"
FT /evidence="ECO:0000255"
FT DOMAIN 688..766
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 785..862
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 1..58
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 517..941
FT /note="Necessary and sufficient for U6 snRNA binding"
FT /evidence="ECO:0000250|UniProtKB:Q15020"
FT REGION 567..686
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 905..938
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 533..593
FT /evidence="ECO:0000255"
FT COMPBIAS 11..54
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 567..608
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 617..633
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 951 AA; 109735 MW; DFBD6B293A95B156 CRC64;
MAATGNEEQT LLPDIEEEAE GMEREMESED DEEEGMGVEH SEEEDEEDTS EDERENEAEI
QRLEEQLSIN AFDYNCHVDL IKLLRQEGKL HRLRKARQKM SELFPLTEEI WLDWLKDEIR
ITEDESDREK VYELFERAIK DYVCPEIWLE YVQYSIGGMG AQGGIERVRS IFERALTAVG
LHMTKGASIW EAYREFEIVI LSTVQPPPGT VPSQEQQELL SAQLERIHTL FRRQLAVPLM
DMEGTYAEYS DWADDGVPET VTHQYRRALQ QMEKGKPYEE ALLVSEPPKL AEYQSYIDFE
IKEGDPARVQ IIFERALAEN CLVPDLWIKY TTYLDRQLKI KDLVLSAHER AVRNCPWTMG
LWKSYLLALE RHGADHQTVK DVFEKALNAG FIQATDYVEI WQSYLDYLRR RVDFSKEWSR
ELDELRAAFS RSLEYLKQDV EERFSESGDL SCTLMQIWAR IEALHCKNMQ KARELWDSIM
TKGNAKYANM WLEYYNLERS YGDAAHCRKA LHRAVQCTSD YPEHVCDVLL NFERVEGSLE
DWDAAVQKTE TKLNRVCEQR ARVAEKEALH ARQEEEKAEQ RRKVKADKKA QKKGQKANRT
GDKRKAEDDD EEEWGEEAEL PSKRLRGEDD FDSTVTEELM ETESGLFGRR APPARKTEPP
GFRKNQQGAP EPQRQPHDMP KEQRKDENCV FVSNLTFNME DPEGKLRTLF QGCGTIQQVR
PVFTAKGTFR GYCYVQFEDR LAVPEALKMD RQEVDGRPMY VSPCVDKNKN PDFKVFKYKT
SMEKHKIFIS GLPYSATKET LEDLCKEHGT IRAIRIVTNR SGKSKGLAYV EFEDEAQASQ
AVLKMDGTML ENFTLSVAIS NPPGRRMKDE AAPSRFLGAA MPRQLQGARG KGRTQISLLP
RSLYRQSTPD AKAENGTISA PHATVTDGET SLDTQTKSLS NEDFARMLLK K
//
