ID SCM3_YEAST Reviewed; 223 AA.
AC Q12334; D6VRK9;
DT 03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 2.
DT 27-MAR-2024, entry version 145.
DE RecName: Full=Protein SCM3;
DE AltName: Full=Suppressor of chromosome missegregation protein 3;
GN Name=SCM3; OrderedLocusNames=YDL139C; ORFNames=D2155;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=8972577;
RX DOI=10.1002/(sici)1097-0061(199612)12:15<1549::aid-yea42>3.0.co;2-s;
RA Woelfl S., Haneman V., Saluz H.P.;
RT "Analysis of a 26,756 bp segment from the left arm of yeast chromosome
RT IV.";
RL Yeast 12:1549-1554(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP IDENTIFICATION OF PROBABLE INITIATION SITE.
RX PubMed=12748633; DOI=10.1038/nature01644;
RA Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.;
RT "Sequencing and comparison of yeast species to identify genes and
RT regulatory elements.";
RL Nature 423:241-254(2003).
RN [6]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [7]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [9]
RP INTERACTION WITH YTA7.
RX PubMed=32079723; DOI=10.1073/pnas.1917814117;
RA Shahnejat-Bushehri S., Ehrenhofer-Murray A.E.;
RT "The ATAD2/ANCCA homolog Yta7 cooperates with Scm3HJURP to deposit
RT Cse4CENP-A at the centromere in yeast.";
RL Proc. Natl. Acad. Sci. U.S.A. 117:5386-5393(2020).
CC -!- FUNCTION: Centromeric protein that plays a central role in the
CC incorporation and maintenance of histone H3-like variant CENPA at
CC centromeres. {ECO:0000250|UniProtKB:Q8NCD3}.
CC -!- SUBUNIT: Interacts with YTA7. {ECO:0000269|PubMed:32079723}.
CC -!- INTERACTION:
CC Q12334; P36012: CSE4; NbExp=5; IntAct=EBI-31788, EBI-5182;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 556 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAS56490.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAA65620.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAA98712.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; X96876; CAA65620.1; ALT_INIT; Genomic_DNA.
DR EMBL; Z74187; CAA98712.1; ALT_INIT; Genomic_DNA.
DR EMBL; AY558164; AAS56490.1; ALT_INIT; Genomic_DNA.
DR EMBL; BK006938; DAA11719.1; -; Genomic_DNA.
DR PIR; S67685; S67685.
DR RefSeq; NP_010142.2; NM_001180199.1.
DR PDB; 2L5A; NMR; -; A=93-172.
DR PDBsum; 2L5A; -.
DR AlphaFoldDB; Q12334; -.
DR SMR; Q12334; -.
DR BioGRID; 31922; 150.
DR DIP; DIP-5045N; -.
DR IntAct; Q12334; 9.
DR MINT; Q12334; -.
DR STRING; 4932.YDL139C; -.
DR MaxQB; Q12334; -.
DR PaxDb; 4932-YDL139C; -.
DR PeptideAtlas; Q12334; -.
DR EnsemblFungi; YDL139C_mRNA; YDL139C; YDL139C.
DR GeneID; 851416; -.
DR KEGG; sce:YDL139C; -.
DR AGR; SGD:S000002298; -.
DR SGD; S000002298; SCM3.
DR VEuPathDB; FungiDB:YDL139C; -.
DR eggNOG; ENOG502S4HZ; Eukaryota.
DR HOGENOM; CLU_106374_0_0_1; -.
DR InParanoid; Q12334; -.
DR OMA; WANDSEA; -.
DR OrthoDB; 2788167at2759; -.
DR BioCyc; YEAST:G3O-29538-MONOMER; -.
DR BioGRID-ORCS; 851416; 5 hits in 10 CRISPR screens.
DR PRO; PR:Q12334; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q12334; Protein.
DR GO; GO:0000779; C:condensed chromosome, centromeric region; IDA:SGD.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0097030; F:CENP-A containing nucleosome binding; IMP:SGD.
DR GO; GO:0042393; F:histone binding; IEA:InterPro.
DR GO; GO:0007059; P:chromosome segregation; IMP:SGD.
DR GO; GO:0000086; P:G2/M transition of mitotic cell cycle; IMP:SGD.
DR GO; GO:0051382; P:kinetochore assembly; IMP:SGD.
DR GO; GO:2000059; P:negative regulation of ubiquitin-dependent protein catabolic process; IDA:SGD.
DR GO; GO:0071459; P:protein localization to chromosome, centromeric region; IMP:SGD.
DR Gene3D; 6.10.250.2010; -; 1.
DR InterPro; IPR018465; Scm3/HJURP.
DR Pfam; PF10384; Scm3; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Nucleus; Reference proteome.
FT CHAIN 1..223
FT /note="Protein SCM3"
FT /id="PRO_0000252298"
FT REGION 1..48
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 168..202
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..16
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 23..48
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 186..202
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT HELIX 94..114
FT /evidence="ECO:0007829|PDB:2L5A"
FT STRAND 125..128
FT /evidence="ECO:0007829|PDB:2L5A"
FT HELIX 146..148
FT /evidence="ECO:0007829|PDB:2L5A"
FT HELIX 154..164
FT /evidence="ECO:0007829|PDB:2L5A"
SQ SEQUENCE 223 AA; 25754 MW; D7668117BCC7FF12 CRC64;
MKTNKKISKR RSLKNLHGAL KGLLKESGKK SESKIRKHSD CNPVHRVYPP NIEKRKTKKD
DGISRPIAER NGHVYIMSKE NHIIPKLTDD EVMERHKLAD ENMRKVWSNI ISKYESIEEQ
GDLVDLKTGE IVEDNGHIKT LTANNSTKDK RTKYTSVLRD IIDISDEEDG DKNDEYTLWA
NDSEASDSEV DADNDTEEEK DEKLIDADFK KYEAKLSKRI LRD
//
