ID SLS1_YEAST Reviewed; 643 AA.
AC P42900; D6VYD4; Q6B228;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 27-MAR-2024, entry version 145.
DE RecName: Full=Sigma-like sequence protein 1, mitochondrial;
DE Short=Protein SLS1;
DE Flags: Precursor;
GN Name=SLS1; OrderedLocusNames=YLR139C; ORFNames=L3162;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=8917313; DOI=10.1007/bf02173976;
RA Rouillard J.-M., Dufour M.-E., Theunissen B., Mandart E., Dujardin G.,
RA Lacroute F.;
RT "SLS1, a new Saccharomyces cerevisiae gene involved in mitochondrial
RT metabolism, isolated as a syntheticlethal in association with an SSM4
RT deletion.";
RL Mol. Gen. Genet. 252:700-708(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP FUNCTION.
RX PubMed=11805046; DOI=10.1093/genetics/160.1.75;
RA Bryan A.C., Rodeheffer M.S., Wearn C.M., Shadel G.S.;
RT "Sls1p is a membrane-bound regulator of transcription-coupled processes
RT involved in Saccharomyces cerevisiae mitochondrial gene expression.";
RL Genetics 160:75-82(2002).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Involved in aerobic respiration where it is required for
CC assembly of respiratory chain enzyme complexes III and IV. Also has a
CC role in mitochondrial gene expression. May be part of a mitochondrial
CC membrane-associated RNA-shuttling system, delivering NAM1-associated
CC transcripts to the translation machinery. {ECO:0000269|PubMed:11805046,
CC ECO:0000269|PubMed:8917313}.
CC -!- INTERACTION:
CC P42900; P10849: MTF2; NbExp=3; IntAct=EBI-17364, EBI-11823;
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000269|PubMed:8917313}.
CC -!- MISCELLANEOUS: Present with 3430 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the SLS1 family. {ECO:0000305}.
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DR EMBL; Z48452; CAA88362.1; -; Genomic_DNA.
DR EMBL; X91258; CAA62658.1; -; Genomic_DNA.
DR EMBL; Z73311; CAA97710.1; -; Genomic_DNA.
DR EMBL; U53881; AAB82401.1; -; Genomic_DNA.
DR EMBL; AY692902; AAT92921.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09450.1; -; Genomic_DNA.
DR PIR; S55593; S55593.
DR RefSeq; NP_013240.1; NM_001182026.1.
DR AlphaFoldDB; P42900; -.
DR BioGRID; 31408; 65.
DR IntAct; P42900; 2.
DR MINT; P42900; -.
DR STRING; 4932.YLR139C; -.
DR MaxQB; P42900; -.
DR PaxDb; 4932-YLR139C; -.
DR PeptideAtlas; P42900; -.
DR EnsemblFungi; YLR139C_mRNA; YLR139C; YLR139C.
DR GeneID; 850830; -.
DR KEGG; sce:YLR139C; -.
DR AGR; SGD:S000004129; -.
DR SGD; S000004129; SLS1.
DR VEuPathDB; FungiDB:YLR139C; -.
DR eggNOG; ENOG502QUD1; Eukaryota.
DR HOGENOM; CLU_390400_0_0_1; -.
DR InParanoid; P42900; -.
DR OMA; LWFELDE; -.
DR OrthoDB; 1997037at2759; -.
DR BioCyc; YEAST:G3O-32279-MONOMER; -.
DR BioGRID-ORCS; 850830; 4 hits in 10 CRISPR screens.
DR PRO; PR:P42900; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; P42900; Protein.
DR GO; GO:0016020; C:membrane; IDA:SGD.
DR GO; GO:0005743; C:mitochondrial inner membrane; IDA:SGD.
DR GO; GO:0042645; C:mitochondrial nucleoid; IDA:SGD.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0009060; P:aerobic respiration; IMP:SGD.
DR GO; GO:0032543; P:mitochondrial translation; IMP:SGD.
DR InterPro; IPR048401; SLS1_C.
DR InterPro; IPR032741; Sls1_KH-1.
DR InterPro; IPR048748; SLS1_KH2.
DR InterPro; IPR048400; SLS1_N.
DR Pfam; PF14611; SLS; 1.
DR Pfam; PF20778; SLS1_C; 1.
DR Pfam; PF20777; SLS1_KH2; 1.
DR Pfam; PF20776; SLS1_N; 1.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane;
KW Protein biosynthesis; Reference proteome; Transit peptide.
FT TRANSIT 1..?
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN ?..643
FT /note="Sigma-like sequence protein 1, mitochondrial"
FT /id="PRO_0000022360"
FT REGION 43..72
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 103
FT /note="A -> V (in Ref. 4; AAT92921)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 643 AA; 73257 MW; 6CCD6FE80D669548 CRC64;
MWKFNKKLAR LTYRLYSSSG PSSPLHGKKK LPQNLKFVVL NPTQSGLVKN DQKQPRHRPS
KKRSHKETGD NNLDFGSKLL VFEKQNSLDS ALNSIRLKKP TSASLPSLEY NALLQSLTSS
YNRYQLREFI STHQPDSSSH LTHWKKSKLS QYIIEKIWNC QPISTPTTPT GIKSTSLTFQ
FDSPREIFLL LITQNGKILT NFNKLGLTFI ISIQDNELTV KGSPSLLKYA EISLNKIWSN
ITHENVRMYS LMPSKDVINL IQKETHTFFE YLPDLQMYKI SALSTKKISM AKVFLLNAVA
SNPNTTQHHH TIASPALKTE LYPFNNTLEN LDWLNKSQDW ARLQSVVPKN CTDLMTPTEN
ATPELTDAQV SQFESSLSKN IPSLSPSDSI SQSLSITLGH SLQSASFSSI FQPLIHKSFI
SKLLNLPMYK ESSSSAVPVP VPLDQHLITN AHQSFIQLNF TPVPPTSGSS SSPFLQIWFE
IDEFDNIVTT SMRPLLKLQE NSVILRTPQC QTDYKITSDY IQDLLPDFDQ TNPDAWLSEQ
KGLQEFLLKS HWKLNKYQNL MKKINISLPD NLIQQYQLTD VLTHRVLNLR FPTNTAQDDK
YIIQYSDISR GFLNNGSYRQ LDFINVNPSE TSLKTFINDV LSF
//
