ID SLT1_CHLRE Reviewed; 881 AA.
AC A8IJF8;
DT 16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 1.
DT 27-MAR-2024, entry version 75.
DE RecName: Full=Sodium/sulfate cotransporter 1;
DE AltName: Full=SAC1-like transporter 1;
DE Short=CrSLT1;
GN Name=SLT1; ORFNames=CHLREDRAFT_205502;
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION BY SULFUR, SUBCELLULAR
RP LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=20498339; DOI=10.1104/pp.110.157875;
RA Pootakham W., Gonzalez-Ballester D., Grossman A.R.;
RT "Identification and regulation of plasma membrane sulfate transporters in
RT Chlamydomonas.";
RL Plant Physiol. 153:1653-1668(2010).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC-503;
RX PubMed=17932292; DOI=10.1126/science.1143609;
RA Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J.,
RA Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K., Marechal-Drouard L.,
RA Marshall W.F., Qu L.H., Nelson D.R., Sanderfoot A.A., Spalding M.H.,
RA Kapitonov V.V., Ren Q., Ferris P., Lindquist E., Shapiro H., Lucas S.M.,
RA Grimwood J., Schmutz J., Cardol P., Cerutti H., Chanfreau G., Chen C.L.,
RA Cognat V., Croft M.T., Dent R., Dutcher S., Fernandez E., Fukuzawa H.,
RA Gonzalez-Ballester D., Gonzalez-Halphen D., Hallmann A., Hanikenne M.,
RA Hippler M., Inwood W., Jabbari K., Kalanon M., Kuras R., Lefebvre P.A.,
RA Lemaire S.D., Lobanov A.V., Lohr M., Manuell A., Meier I., Mets L.,
RA Mittag M., Mittelmeier T., Moroney J.V., Moseley J., Napoli C.,
RA Nedelcu A.M., Niyogi K., Novoselov S.V., Paulsen I.T., Pazour G.J.,
RA Purton S., Ral J.P., Riano-Pachon D.M., Riekhof W., Rymarquis L.,
RA Schroda M., Stern D., Umen J., Willows R., Wilson N., Zimmer S.L.,
RA Allmer J., Balk J., Bisova K., Chen C.J., Elias M., Gendler K., Hauser C.,
RA Lamb M.R., Ledford H., Long J.C., Minagawa J., Page M.D., Pan J.,
RA Pootakham W., Roje S., Rose A., Stahlberg E., Terauchi A.M., Yang P.,
RA Ball S., Bowler C., Dieckmann C.L., Gladyshev V.N., Green P., Jorgensen R.,
RA Mayfield S., Mueller-Roeber B., Rajamani S., Sayre R.T., Brokstein P.,
RA Dubchak I., Goodstein D., Hornick L., Huang Y.W., Jhaveri J., Luo Y.,
RA Martinez D., Ngau W.C., Otillar B., Poliakov A., Porter A., Szajkowski L.,
RA Werner G., Zhou K., Grigoriev I.V., Rokhsar D.S., Grossman A.R.;
RT "The Chlamydomonas genome reveals the evolution of key animal and plant
RT functions.";
RL Science 318:245-250(2007).
RN [3]
RP INDUCTION BY SULFUR.
RX PubMed=18326790; DOI=10.1104/pp.108.116137;
RA Gonzalez-Ballester D., Pollock S.V., Pootakham W., Grossman A.R.;
RT "The central role of a SNRK2 kinase in sulfur deprivation responses.";
RL Plant Physiol. 147:216-227(2008).
CC -!- FUNCTION: Na(+)/sulfate cotransporter with a probable high-affinity for
CC sulfate and a proteasome dependent turnover.
CC {ECO:0000269|PubMed:20498339}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:20498339};
CC Multi-pass membrane protein {ECO:0000269|PubMed:20498339}.
CC -!- INDUCTION: Up-regulated by sulfur deprivation.
CC {ECO:0000269|PubMed:18326790, ECO:0000269|PubMed:20498339}.
CC -!- DISRUPTION PHENOTYPE: No effect on sulfate uptake, due to the
CC redundancy with SLT2 and SULTR2. Slt1 and slt2, as well as slt1 and
CC sultr2 double mutants show a 50% decline in uptake while the slt1, slt2
CC and sultr2 triple mutant exhibits no increase in sulfate uptake
CC capacity when deprived of sulfur. {ECO:0000269|PubMed:20498339}.
CC -!- SIMILARITY: Belongs to the divalent anion:Na+ symporter (DASS)
CC superfamily. Na+/sulfate symporter (TC 2.A.47.4) family. {ECO:0000305}.
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DR EMBL; GU181275; ACZ63168.1; -; mRNA.
DR EMBL; DS496118; EDP05421.1; -; Genomic_DNA.
DR RefSeq; XP_001690975.1; XM_001690923.1.
DR AlphaFoldDB; A8IJF8; -.
DR TCDB; 2.A.47.4.3; the divalent anion:na(+) symporter (dass) family.
DR PaxDb; 3055-EDP05421; -.
DR EnsemblPlants; PNW74959; PNW74959; CHLRE_12g502600v5.
DR GeneID; 5716413; -.
DR Gramene; PNW74959; PNW74959; CHLRE_12g502600v5.
DR KEGG; cre:CHLRE_12g502600v5; -.
DR eggNOG; ENOG502QPZM; Eukaryota.
DR HOGENOM; CLU_005170_6_0_1; -.
DR OMA; SVGMAEF; -.
DR OrthoDB; 276814at2759; -.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0008324; F:monoatomic cation transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015116; F:sulfate transmembrane transporter activity; IMP:UniProtKB.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0006813; P:potassium ion transport; IEA:InterPro.
DR Gene3D; 3.30.70.1450; Regulator of K+ conductance, C-terminal domain; 3.
DR InterPro; IPR004680; Cit_transptr-like_dom.
DR InterPro; IPR006037; RCK_C.
DR InterPro; IPR036721; RCK_C_sf.
DR PANTHER; PTHR43652; BASIC AMINO ACID ANTIPORTER YFCC-RELATED; 1.
DR PANTHER; PTHR43652:SF9; NA+_DICARBOXYLATE; 1.
DR Pfam; PF03600; CitMHS; 1.
DR Pfam; PF02080; TrkA_C; 3.
DR SUPFAM; SSF116726; TrkA C-terminal domain-like; 4.
DR PROSITE; PS51202; RCK_C; 4.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Repeat; Stress response; Sulfate transport;
KW Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..881
FT /note="Sodium/sulfate cotransporter 1"
FT /id="PRO_0000417124"
FT TRANSMEM 8..28
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 31..51
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 61..81
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 107..127
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 186..206
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 601..621
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 625..645
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 658..678
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 684..704
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 775..795
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 803..823
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 212..296
FT /note="RCK C-terminal 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00544"
FT DOMAIN 318..402
FT /note="RCK C-terminal 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00544"
FT DOMAIN 407..492
FT /note="RCK C-terminal 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00544"
FT DOMAIN 498..584
FT /note="RCK C-terminal 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00544"
FT REGION 854..881
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 881 AA; 94832 MW; 608A1775884F8DFA CRC64;
MAALSWQGIV AVTFTALAFV VMAADWVGPD ITFTVLLAFL TAFDGQIVTV AKAAAGYGNT
GLLTVVFLYW VAEGITQTGG LELIMNYVLG RSRSVHWALV RSMFPVMVLS AFLNNTPCVT
FMIPILISWG RRCGVPIKKL LIPLSYAAVL GGTCTSIGTS TNLVIVGLQD ARYAKSKQVD
QAKFQIFDIA PYGVPYALWG FVFILLAQGF LLPGNSSRYA KDLLLAVRVL PSSSVVKKKL
KDSGLLQQNG FDVTAIYRNG QLIKISDPSI VLDGGDILYV SGELDVVEFV GEEYGLALVN
QEQELAAERP FGSGEEAVFS ANGAAPYHKL VQAKLSKTSD LIGRTVREVS WQGRFGLIPV
AIQRGNGRED GRLSDVVLAA GDVLLLDTTP FYDEDREDIK TNFDGKLHAV KDGAAKEFVI
GVKVKKSAEV VGKTVSAAGL RGIPGLFVLS VDHADGTSVD SSDYLYKIQP DDTIWIAADV
AAVGFLSKFP GLELVQQEQV DKTGTSILYR HLVQAAVSHK GPLVGKTVRD VRFRTLYNAA
VVAVHRENAR IPLKVQDIVL QGGDVLLISC HTNWADEHRH DKSFVLVQPV PDSSPPKRSR
MIIGVLLATG MVLTQIIGGL KNKEYIHLWP CAVLTAALML LTGCMNADQT RKAIMWDVYL
TIAAAFGVSA ALEGTGVAAK FANAIISIGK GAGGTGAALI AIYIATALLS ELLTNNAAGA
IMYPIAAIAG DALKITPKDT SVAIMLGASA GFVNPFSYQT NLMVYAAGNY SVREFAIVGA
PFQVWLMIVA GFILVYRNQW HQVWIVSWIC TAGIVLLPAL YFLLPTRIQI KIDGFFERIA
AVLNPKAALE RRRSLRRQVS HTRTDDSGSS GSPLPAPKIV A
//
