ID SPSA1_ORYSJ Reviewed; 1084 AA.
AC Q0JGK4; Q43010; Q43802; Q5JLN3; Q94JN0;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-SEP-2007, sequence version 2.
DT 24-JAN-2024, entry version 94.
DE RecName: Full=Probable sucrose-phosphate synthase 1;
DE EC=2.4.1.14;
DE AltName: Full=Sucrose phosphate synthase 1F;
DE Short=OsSPS1F;
DE AltName: Full=UDP-glucose-fructose-phosphate glucosyltransferase;
GN Name=SPS1; OrderedLocusNames=Os01g0919400, LOC_Os01g69030;
GN ORFNames=P0678F11.16;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RA Sakamoto M., Satozawa T., Kishimoto N., Higo K., Shimada H., Fujimura T.;
RT "Structure and RFLP mapping of a rice sucrose phosphate synthase (SPS) gene
RT that is specifically expressed in the source organ.";
RL Plant Sci. 112:207-217(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 102-199.
RC STRAIN=cv. Stejaree45;
RA Lee D.-S., Hur Y.;
RT "Expression and regulation of genes involved in carbohydrate metabolism in
RT rice.";
RL Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP GENE FAMILY.
RX PubMed=16876912; DOI=10.1016/j.jplph.2006.04.014;
RA Lutfiyya L.L., Xu N., D'Ordine R.L., Morrell J.A., Miller P.W., Duff S.M.;
RT "Phylogenetic and expression analysis of sucrose phosphate synthase
RT isozymes in plants.";
RL J. Plant Physiol. 164:923-933(2007).
RN [8]
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND INDUCTION.
RX PubMed=21683881; DOI=10.1016/j.plantsci.2011.04.019;
RA Okamura M., Aoki N., Hirose T., Yonekura M., Ohto C., Ohsugi R.;
RT "Tissue specificity and diurnal change in gene expression of the sucrose
RT phosphate synthase gene family in rice.";
RL Plant Sci. 181:159-166(2011).
CC -!- FUNCTION: Plays a role in photosynthetic sucrose synthesis by
CC catalyzing the rate-limiting step of sucrose biosynthesis from UDP-
CC glucose and fructose- 6-phosphate. Involved in the regulation of carbon
CC partitioning in the leaves of plants. May regulate the synthesis of
CC sucrose and therefore play a major role as a limiting factor in the
CC export of photoassimilates out of the leaf. Plays a role for sucrose
CC availability that is essential for plant growth and fiber elongation
CC (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=beta-D-fructose 6-phosphate + UDP-alpha-D-glucose = H(+) +
CC sucrose 6(F)-phosphate + UDP; Xref=Rhea:RHEA:22172,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57634, ChEBI:CHEBI:57723,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:58885; EC=2.4.1.14;
CC -!- ACTIVITY REGULATION: Activity is regulated by phosphorylation and
CC moderated by concentration of metabolites and light. {ECO:0000250}.
CC -!- PATHWAY: Glycan biosynthesis; sucrose biosynthesis; sucrose from D-
CC fructose 6-phosphate and UDP-alpha-D-glucose: step 1/2.
CC -!- SUBUNIT: Homodimer or homotetramer. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in germinating seeds.
CC {ECO:0000269|PubMed:21683881}.
CC -!- DEVELOPMENTAL STAGE: Highly expressed in source leaves and at low
CC levels in sink leaves. {ECO:0000269|PubMed:21683881}.
CC -!- INDUCTION: Circadian-regulated, with the highest expression 1 hour
CC before the beginning of light period (in 14 hours light/10 hours dark
CC cycle). {ECO:0000269|PubMed:21683881}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF07124.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; D45890; BAA08304.1; -; Genomic_DNA.
DR EMBL; AP003437; BAD87626.1; -; Genomic_DNA.
DR EMBL; AP008207; BAF07124.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014957; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AF378184; AAK54855.1; -; mRNA.
DR PIR; T04103; T04103.
DR RefSeq; XP_015621705.1; XM_015766219.1.
DR RefSeq; XP_015621706.1; XM_015766220.1.
DR AlphaFoldDB; Q0JGK4; -.
DR SMR; Q0JGK4; -.
DR STRING; 39947.Q0JGK4; -.
DR CAZy; GT4; Glycosyltransferase Family 4.
DR PaxDb; 39947-Q0JGK4; -.
DR EnsemblPlants; Os01t0919400-01; Os01t0919400-01; Os01g0919400.
DR GeneID; 4324364; -.
DR Gramene; Os01t0919400-01; Os01t0919400-01; Os01g0919400.
DR KEGG; osa:4324364; -.
DR eggNOG; KOG0853; Eukaryota.
DR InParanoid; Q0JGK4; -.
DR OrthoDB; 1206157at2759; -.
DR BRENDA; 2.4.1.14; 8948.
DR PlantReactome; R-OSA-1119465; Sucrose biosynthesis.
DR UniPathway; UPA00371; UER00545.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR GO; GO:0005794; C:Golgi apparatus; IEA:EnsemblPlants.
DR GO; GO:0016157; F:sucrose synthase activity; IEA:InterPro.
DR GO; GO:0046524; F:sucrose-phosphate synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0005986; P:sucrose biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd03800; GT4_sucrose_synthase; 1.
DR CDD; cd16419; HAD_SPS; 1.
DR Gene3D; 3.40.50.2000; Glycogen Phosphorylase B; 2.
DR InterPro; IPR001296; Glyco_trans_1.
DR InterPro; IPR006380; SPP-like_dom.
DR InterPro; IPR035659; SPS_C.
DR InterPro; IPR012819; SPS_pln.
DR InterPro; IPR000368; Sucrose_synth.
DR NCBIfam; TIGR02468; sucrsPsyn_pln; 1.
DR PANTHER; PTHR46039; SUCROSE-PHOSPHATE SYNTHASE 3-RELATED; 1.
DR PANTHER; PTHR46039:SF5; SUCROSE-PHOSPHATE SYNTHASE 3-RELATED; 1.
DR Pfam; PF00534; Glycos_transf_1; 1.
DR Pfam; PF05116; S6PP; 1.
DR Pfam; PF00862; Sucrose_synth; 1.
DR SUPFAM; SSF53756; UDP-Glycosyltransferase/glycogen phosphorylase; 1.
PE 2: Evidence at transcript level;
KW Glycosyltransferase; Reference proteome; Transferase.
FT CHAIN 1..1084
FT /note="Probable sucrose-phosphate synthase 1"
FT /id="PRO_0000204672"
FT REGION 25..61
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 49
FT /note="R -> S (in Ref. 1; BAA08304)"
FT /evidence="ECO:0000305"
FT CONFLICT 52..56
FT /note="AAGAA -> TTGTT (in Ref. 1; BAA08304)"
FT /evidence="ECO:0000305"
FT CONFLICT 384
FT /note="I -> M (in Ref. 1; BAA08304)"
FT /evidence="ECO:0000305"
FT CONFLICT 494
FT /note="M -> S (in Ref. 1; BAA08304)"
FT /evidence="ECO:0000305"
FT CONFLICT 497
FT /note="L -> W (in Ref. 1; BAA08304)"
FT /evidence="ECO:0000305"
FT CONFLICT 539
FT /note="I -> S (in Ref. 1; BAA08304)"
FT /evidence="ECO:0000305"
FT CONFLICT 542
FT /note="N -> T (in Ref. 1; BAA08304)"
FT /evidence="ECO:0000305"
FT CONFLICT 548
FT /note="E -> G (in Ref. 1; BAA08304)"
FT /evidence="ECO:0000305"
FT CONFLICT 578
FT /note="H -> Y (in Ref. 1; BAA08304)"
FT /evidence="ECO:0000305"
FT CONFLICT 623
FT /note="A -> G (in Ref. 1; BAA08304)"
FT /evidence="ECO:0000305"
FT CONFLICT 767
FT /note="D -> E (in Ref. 1; BAA08304)"
FT /evidence="ECO:0000305"
FT CONFLICT 950
FT /note="I -> M (in Ref. 1; BAA08304)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1084 AA; 119348 MW; A42BA599C4DF4556 CRC64;
MAGNEWINGY LEAILDSGGA AGGGGGGGGG GGGGGGGGGG GGGGGVDPRS PAAGAASPRG
PHMNFNPTHY FVEEVVKGVD ESDLHRTWIK VVATRNARER STRLENMCWR IWHLARKKKQ
LELEGILRIS ARRKEQEQVR RETSEDLAED LFEGEKADTV GELAQQDTPM KKKFQRNFSE
LTVSWSDENK EKKLYIVLIS LHGLVRGDNM ELGRDSDTGG QVKYVVELAR ALAMMPGVYR
VDLFTRQVSS PEVDWSYGEP TEMLTSGSTD GEGSGESAGA YIVRIPCGPR DKYLRKEALW
PYLQEFVDGA LAHILNMSKA LGEQVSNGKL VLPYVIHGHY ADAGDVAALL SGALNVPMVL
TGHSLGRNKL EQIMKQGRMS KEEIDSTYKI MRRIEGEELA LDAAELVITS TRQEIDEQWG
LYDGFDVKLE KVLRARARRG VSCHGRFMPR MVVIPPGMDF SSVVVPEDTS DGDDGKDFEI
ASPRSLPPIW AEVMRFLTNP HKPMILALSR PDPKKNITTL VKAFGECRPL RELANLILIM
GNRDDIDEMS AGNASVLTTV LKLIDKYDLY GSVAFPKHHK QSDVPEIYRL TGKMKGVFIN
PALVEPFGLT LIEAAAHGLP IVATKNGGPV DIKNALNNGL LVDPHDQHAI ADALLKLVAD
KNLWQECRKN GLRNIQLYSW PEHCRTYLTR IAGCRIRNPR WLMDTPADAA AEEEEALEDS
LMDVQDLSLR LSIDGERGSS MNDAPSSDPQ DSVQRIMNKI KRSSPADTDG AKIPAEAAAT
ATSGAMNKYP LLRRRRRLFV IAVDCYGDDG SASKRMLQVI QEVFRAVRSD SQMSRISGFA
LSTAMPLPET LKLLQLGKIP PTDFDALICG SGSEVYYPST AQCVDAGGRL RPDQDYLLHI
NHRWSHDGAK QTIAKLAHDG SGTNVEPDVE SCNPHCVSFF IKDPNKVRTI DEMRERVRMR
GLRCHLMYCR NATRLQVVPL LASRSQALRY LFVRWGLSVG NMYLIVGEHG DTDHEEMLSG
LHKTVIIRGV TEKGSEQLVR SSGSYQREDV VPSESPLIAF TKGDLKADEI MRALKEVTKA
ASGM
//
