ID SUS1_ORYSJ Reviewed; 816 AA.
AC P31924; A0A0P0VZ94; F4MFT0; Q10K07; Q10K08; Q7G7N0;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1993, sequence version 1.
DT 24-JAN-2024, entry version 133.
DE RecName: Full=Sucrose synthase 1;
DE Short=OsSUS1;
DE EC=2.4.1.13;
DE AltName: Full=Sucrose synthase 2;
DE Short=RSs2;
DE AltName: Full=Sucrose-UDP glucosyltransferase 1;
GN Name=SUS1; Synonyms=RSS2, RSUS1;
GN OrderedLocusNames=Os03g0401300, LOC_Os03g28330; ORFNames=OsJ_11182;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Tainung 67; TISSUE=Seed;
RX PubMed=1531032; DOI=10.1007/bf00018467;
RA Yu W.P., Wang A.Y., Juang R.H., Sung H.Y., Su J.C.;
RT "Isolation and sequences of rice sucrose synthase cDNA and genomic DNA.";
RL Plant Mol. Biol. 18:139-142(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Tainung 67; TISSUE=Seed;
RX PubMed=9063969; DOI=10.1271/bbb.60.233;
RA Huang J.W., Chen J.T., Yu W.P., Shyur L.F., Wang A.Y., Sung H.Y., Lee P.D.,
RA Su J.C.;
RT "Complete structures of three rice sucrose synthase isogenes and
RT differential regulation of their expressions.";
RL Biosci. Biotechnol. Biochem. 60:233-239(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Nipponbare;
RX PubMed=21533550; DOI=10.1007/s10059-011-1038-y;
RA Cho J.I., Kim H.B., Kim C.Y., Hahn T.R., Jeon J.S.;
RT "Identification and characterization of the duplicate rice sucrose synthase
RT genes OsSUS5 and OsSUS7 which are associated with the plasma membrane.";
RL Mol. Cells 31:553-561(2011).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [7]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [9]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [10]
RP SUBUNIT.
RX PubMed=9784845; DOI=10.1080/15216549800203612;
RA Huang D.Y., Wang A.-Y.;
RT "Purification and characterization of sucrose synthase isozymes from
RT etiolated rice seedlings.";
RL Biochem. Mol. Biol. Int. 46:107-113(1998).
RN [11]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=10555303; DOI=10.1093/oxfordjournals.pcp.a029608;
RA Wang A.-Y., Kao M.-H., Yang W.-H., Sayion Y., Liu L.-F., Lee P.-D.,
RA Su J.-C.;
RT "Differentially and developmentally regulated expression of three rice
RT sucrose synthase genes.";
RL Plant Cell Physiol. 40:800-807(1999).
RN [12]
RP INDUCTION BY SUGARS.
RA Liao Y.-C., Wang A.-Y.;
RT "Sugar-modulated gene expression of sucrose synthase in suspension-cultured
RT cells of rice.";
RL Physiol. Plantarum 118:319-327(2003).
RN [13]
RP DEVELOPMENTAL STAGE.
RX PubMed=15701658; DOI=10.1093/pcp/pci066;
RA Ishimaru T., Hirose T., Matsuda T., Goto A., Takahashi K., Sasaki H.,
RA Terao T., Ishii R., Ohsugi R., Yamagishi T.;
RT "Expression patterns of genes encoding carbohydrate-metabolizing enzymes
RT and their relationship to grain filling in rice (Oryza sativa L.):
RT comparison of caryopses located at different positions in a panicle.";
RL Plant Cell Physiol. 46:620-628(2005).
RN [14]
RP INDUCTION BY CO2.
RX PubMed=18713413; DOI=10.1111/j.1744-7909.2008.00666.x;
RA Li J.-Y., Liu X.-H., Cai Q.-S., Gu H., Zhang S.-S., Wu Y.-Y., Wang C.-J.;
RT "Effects of elevated CO2 on growth, carbon assimilation, photosynthate
RT accumulation and related enzymes in rice leaves during sink-source
RT transition.";
RL J. Integr. Plant Biol. 50:723-732(2008).
RN [15]
RP GENE FAMILY, AND TISSUE SPECIFICITY.
RX DOI=10.1016/j.plantsci.2008.02.009;
RA Hirose T., Scofield G.N., Terao T.;
RT "An expression analysis profile for the entire sucrose synthase gene family
RT in rice.";
RL Plant Sci. 174:534-543(2008).
CC -!- FUNCTION: Sucrose-cleaving enzyme that provides UDP-glucose and
CC fructose for various metabolic pathways. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an NDP-alpha-D-glucose + D-fructose = a ribonucleoside 5'-
CC diphosphate + H(+) + sucrose; Xref=Rhea:RHEA:16241,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17992, ChEBI:CHEBI:37721,
CC ChEBI:CHEBI:57930, ChEBI:CHEBI:76533; EC=2.4.1.13;
CC -!- SUBUNIT: Homotetramer or heterotetramer with SUS2.
CC {ECO:0000269|PubMed:9784845}.
CC -!- TISSUE SPECIFICITY: Expressed in root phloem and leaf mesophyll.
CC Expressed in phloem tissues and aleurone layers of seeds and at lower
CC levels in the pericarp and endosperm cells (at protein level).
CC Predominantly expressed in elongating tissues including roots,
CC developing leaves and internodes. {ECO:0000269|PubMed:10555303,
CC ECO:0000269|Ref.15}.
CC -!- DEVELOPMENTAL STAGE: Expressed early in seed development and then
CC declines gradually and disappears (at protein level). In the caryopse,
CC strongly expressed during the pre-storage phase, and the expression
CC decreased thereafter. {ECO:0000269|PubMed:10555303,
CC ECO:0000269|PubMed:15701658, ECO:0000269|PubMed:21533550}.
CC -!- INDUCTION: Up-regulated by sucrose, fructose and glucose. Up-regulated
CC under elevated CO2 condition. {ECO:0000269|PubMed:18713413,
CC ECO:0000269|Ref.12}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 1 family. Plant sucrose
CC synthase subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABF96469.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; X59046; CAA41774.1; -; Genomic_DNA.
DR EMBL; HQ895719; AEX32874.1; -; mRNA.
DR EMBL; AC084380; AAK52129.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF96467.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF96468.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF96469.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008209; BAF12233.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS84604.1; -; Genomic_DNA.
DR EMBL; CM000140; EAZ27243.1; -; Genomic_DNA.
DR EMBL; AK100334; BAG94557.1; -; mRNA.
DR PIR; S19139; S19139.
DR RefSeq; XP_015629843.1; XM_015774357.1.
DR RefSeq; XP_015629844.1; XM_015774358.1.
DR AlphaFoldDB; P31924; -.
DR SMR; P31924; -.
DR STRING; 39947.P31924; -.
DR CAZy; GT4; Glycosyltransferase Family 4.
DR PaxDb; 39947-P31924; -.
DR EnsemblPlants; Os03t0401300-01; Os03t0401300-01; Os03g0401300.
DR GeneID; 4333062; -.
DR Gramene; Os03t0401300-01; Os03t0401300-01; Os03g0401300.
DR KEGG; osa:4333062; -.
DR eggNOG; KOG0853; Eukaryota.
DR HOGENOM; CLU_019158_1_0_1; -.
DR InParanoid; P31924; -.
DR OMA; NNEHKFM; -.
DR OrthoDB; 1201243at2759; -.
DR BRENDA; 2.4.1.13; 4460.
DR PlantReactome; R-OSA-1119452; Galactose degradation II.
DR PlantReactome; R-OSA-1119465; Sucrose biosynthesis.
DR SABIO-RK; P31924; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000007752; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR ExpressionAtlas; P31924; baseline and differential.
DR Genevisible; P31924; OS.
DR GO; GO:0016157; F:sucrose synthase activity; IBA:GO_Central.
DR GO; GO:0010037; P:response to carbon dioxide; IEP:UniProtKB.
DR GO; GO:0005985; P:sucrose metabolic process; IEA:InterPro.
DR Gene3D; 1.20.120.1230; -; 1.
DR Gene3D; 3.10.450.330; -; 1.
DR Gene3D; 3.40.50.2000; Glycogen Phosphorylase B; 2.
DR InterPro; IPR001296; Glyco_trans_1.
DR InterPro; IPR000368; Sucrose_synth.
DR InterPro; IPR012820; Sucrose_synthase_pln/cyn.
DR NCBIfam; TIGR02470; sucr_synth; 1.
DR PANTHER; PTHR45839; -; 1.
DR PANTHER; PTHR45839:SF29; SUCROSE SYNTHASE 1; 1.
DR Pfam; PF00534; Glycos_transf_1; 1.
DR Pfam; PF00862; Sucrose_synth; 1.
DR SUPFAM; SSF53756; UDP-Glycosyltransferase/glycogen phosphorylase; 1.
PE 1: Evidence at protein level;
KW Glycosyltransferase; Reference proteome; Transferase.
FT CHAIN 1..816
FT /note="Sucrose synthase 1"
FT /id="PRO_0000204656"
FT REGION 280..757
FT /note="GT-B glycosyltransferase"
FT /evidence="ECO:0000250|UniProtKB:P49040"
SQ SEQUENCE 816 AA; 92908 MW; 56704F0B8BEBA947 CRC64;
MGEAAGDRVL SRLHSVRERI GDSLSAHPNE LVAVFTRLVN LGKGMLQAHQ IIAEYNNAIS
EADREKLKDG AFEDVLRSAQ EGIVISPWVA LAIRPRPGVW EYVRVNVSEL AVELLTVPEY
LQFKEQLVEE GTNNNFVLEL DFEPFNASFP RPSLSKSIGN GVQFLNRHLS SKLFHDKESM
YPLLNFLRAH NYKGMTMMLN DRIRSLSALQ GALRKAEEHL SGLSADTPYS EFHHRFQELG
LEKGWGDCAK RSQETIHLLL DLLEAPDPST LEKFLGTIPM VFNVVIMSPH GYFAQANVLG
YPDTGGQVVY ILDQVRAMEN EMLLRIKQQG LNITPRILIV TRLLPDATGT TCGQRLEKVL
GTEHTHILRV PFRTENGIVR KWISRFEVWP YLETFTDDVA HEIAGELQAN PDLIIGNYSD
GNLVACLLAH KMGVTHCTIA HALEKTKYPN SDLYWKKFED HYHFSCQFTT DLIAMNHADF
IITSTFQEIA GNKDTVGQYE SHMAFTMPGL YRVVHGIDVF DPKFNIVSPG ADMSIYFPYS
ESRKRLTSLH PEIEELLYSE VDNNEHKFML KDRNKPIIFS MARLDRVKNL TGLVELYGRN
PRLQELVNLV VVCGDHGNPS KDKEEQAEFK KMFDLIEQYN LNGHIRWISA QMNRVRNGEL
YRYICDTKGA FVQPAFYEAF GLTVVESMTC GLPTFATAYG GPAEIIVNGV SGFHIDPYQG
DKASALLVEF FEKCQEDPSH WTKISQGGLQ RIEEKYTWKL YSERLMTLTG VYGFWKYVSN
LERRETRRYL EMLYALKYRT MASTVPLAVE GEPSNK
//
