ID SWI3B_ARATH Reviewed; 469 AA.
AC Q84JG2; O22811;
DT 22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 27-MAR-2024, entry version 152.
DE RecName: Full=SWI/SNF complex subunit SWI3B;
DE Short=AtSWI3B;
DE AltName: Full=Transcription regulatory protein SWI3B;
GN Name=SWI3B; Synonyms=CHB2; OrderedLocusNames=At2g33610; ORFNames=F4P9.38;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, TISSUE
RP SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH SWI3A; SWI3C; SWI3D;
RP BSH AND FCA, AND SUBUNIT.
RX PubMed=16055636; DOI=10.1105/tpc.105.031203;
RA Sarnowski T.J., Rios G., Jasik J., Swiezewski S., Kaczanowski S., Li Y.,
RA Kwiatkowska A., Pawlikowska K., Kozbial M., Kozbial P., Koncz C.,
RA Jerzmanowski A.;
RT "SWI3 subunits of putative SWI/SNF chromatin-remodeling complexes play
RT distinct roles during Arabidopsis development.";
RL Plant Cell 17:2454-2472(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION, INTERACTION WITH SWI3A; SWI3C; BSH AND FCA, AND SUBUNIT.
RX PubMed=12140326; DOI=10.1093/nar/gkf458;
RA Sarnowski T.J., Swiezewski S., Pawlikowska K., Kaczanowski S.,
RA Jerzmanowski A.;
RT "AtSWI3B, an Arabidopsis homolog of SWI3, a core subunit of yeast Swi/Snf
RT chromatin remodeling complex, interacts with FCA, a regulator of flowering
RT time.";
RL Nucleic Acids Res. 30:3412-3421(2002).
RN [6]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=14682613; DOI=10.1023/b:plan.0000004305.60407.8b;
RA Zhou C., Miki B., Wu K.;
RT "CHB2, a member of the SWI3 gene family, is a global regulator in
RT Arabidopsis.";
RL Plant Mol. Biol. 52:1125-1134(2003).
RN [7]
RP INTERACTION WITH BRM.
RX PubMed=16845477; DOI=10.1007/s11103-006-9021-2;
RA Hurtado L., Farrona S., Reyes J.C.;
RT "The putative SWI/SNF complex subunit BRAHMA activates flower homeotic
RT genes in Arabidopsis thaliana.";
RL Plant Mol. Biol. 62:291-304(2006).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH HAB1.
RX PubMed=19033529; DOI=10.1105/tpc.107.056705;
RA Saez A., Rodrigues A., Santiago J., Rubio S., Rodriguez P.L.;
RT "HAB1-SWI3B interaction reveals a link between abscisic acid signaling and
RT putative SWI/SNF chromatin-remodeling complexes in Arabidopsis.";
RL Plant Cell 20:2972-2988(2008).
RN [9]
RP INTERACTION WITH MORC6 AND SUVH9.
RX PubMed=27171427; DOI=10.1371/journal.pgen.1006026;
RA Liu Z.-W., Zhou J.-X., Huang H.-W., Li Y.-Q., Shao C.-R., Li L., Cai T.,
RA Chen S., He X.-J.;
RT "Two components of the RNA-Directed DNA methylation pathway associate with
RT MORC6 and silence loci targeted by MORC6 in Arabidopsis.";
RL PLoS Genet. 12:E1006026-E1006026(2016).
CC -!- FUNCTION: Component of a multiprotein complex equivalent of the SWI/SNF
CC complex, an ATP-dependent chromatin-remodeling complex, which is
CC required for the positive and negative regulation of gene expression of
CC a large number of genes. It changes chromatin structure by altering
CC DNA-histone contacts within a nucleosome, leading eventually to a
CC change in nucleosome position, thus facilitating or repressing binding
CC of gene-specific transcription factors. May play an essential role in
CC the transition from the vegetative to the reproductive phase of
CC development. May be a positive regulator of ABA signaling.
CC {ECO:0000269|PubMed:12140326, ECO:0000269|PubMed:14682613,
CC ECO:0000269|PubMed:16055636, ECO:0000269|PubMed:19033529}.
CC -!- SUBUNIT: Homodimers and heterodimers. Interacts with SWI3A, SWI3C,
CC SWI3D, BSH, BRM and FCA (via C-terminus), and (via N-terminus) with
CC HAB1. Interacts with MORC6 and SUVH9 (PubMed:27171427).
CC {ECO:0000269|PubMed:12140326, ECO:0000269|PubMed:16055636,
CC ECO:0000269|PubMed:16845477, ECO:0000269|PubMed:19033529,
CC ECO:0000269|PubMed:27171427}.
CC -!- INTERACTION:
CC Q84JG2; O04719: ABI2; NbExp=2; IntAct=EBI-1102271, EBI-537680;
CC Q84JG2; Q9ASZ1: GEBP; NbExp=3; IntAct=EBI-1102271, EBI-15191723;
CC Q84JG2; Q8VYD2: GPL1; NbExp=3; IntAct=EBI-1102271, EBI-4426914;
CC Q84JG2; Q9CAJ0: HAB1; NbExp=4; IntAct=EBI-1102271, EBI-2309302;
CC Q84JG2; P46639: KNAT1; NbExp=6; IntAct=EBI-1102271, EBI-530486;
CC Q84JG2; Q9XI07: SWI3C; NbExp=4; IntAct=EBI-1102271, EBI-1102300;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00624,
CC ECO:0000269|PubMed:16055636, ECO:0000269|PubMed:19033529}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, stems, leaves, flowers and
CC siliques. {ECO:0000269|PubMed:14682613, ECO:0000269|PubMed:16055636}.
CC -!- DISRUPTION PHENOTYPE: Plants have pleiotropic developmental
CC abnormalities including embryo development blocked at the early
CC globular stage. {ECO:0000269|PubMed:16055636}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB80676.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC002332; AAB80676.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC08859.1; -; Genomic_DNA.
DR EMBL; BT004085; AAO42112.1; -; mRNA.
DR EMBL; BT005094; AAO50627.1; -; mRNA.
DR PIR; F84747; F84747.
DR RefSeq; NP_180919.2; NM_128921.6.
DR AlphaFoldDB; Q84JG2; -.
DR SMR; Q84JG2; -.
DR BioGRID; 3274; 152.
DR ComplexPortal; CPX-7727; MINU1/2-associated SWI/SNF ATP-dependent chromatin remodeling complex.
DR IntAct; Q84JG2; 28.
DR STRING; 3702.Q84JG2; -.
DR iPTMnet; Q84JG2; -.
DR PaxDb; 3702-AT2G33610-1; -.
DR ProteomicsDB; 226798; -.
DR EnsemblPlants; AT2G33610.1; AT2G33610.1; AT2G33610.
DR GeneID; 817927; -.
DR Gramene; AT2G33610.1; AT2G33610.1; AT2G33610.
DR KEGG; ath:AT2G33610; -.
DR Araport; AT2G33610; -.
DR TAIR; AT2G33610; SWI3B.
DR eggNOG; KOG1279; Eukaryota.
DR HOGENOM; CLU_004447_5_0_1; -.
DR InParanoid; Q84JG2; -.
DR OMA; EESPCTI; -.
DR OrthoDB; 10062at2759; -.
DR PhylomeDB; Q84JG2; -.
DR PRO; PR:Q84JG2; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q84JG2; baseline and differential.
DR Genevisible; Q84JG2; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0016514; C:SWI/SNF complex; ISS:TAIR.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006338; P:chromatin remodeling; ISS:TAIR.
DR CDD; cd00167; SANT; 1.
DR Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR InterPro; IPR017884; SANT_dom.
DR InterPro; IPR032451; SMARCC_C.
DR InterPro; IPR007526; SWIRM.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR12802; SWI/SNF COMPLEX-RELATED; 1.
DR PANTHER; PTHR12802:SF44; SWI_SNF COMPLEX SUBUNIT SWI3B; 1.
DR Pfam; PF00249; Myb_DNA-binding; 1.
DR Pfam; PF04433; SWIRM; 1.
DR Pfam; PF16495; SWIRM-assoc_1; 1.
DR SMART; SM00717; SANT; 1.
DR SUPFAM; SSF46689; Homeodomain-like; 2.
DR PROSITE; PS51293; SANT; 1.
DR PROSITE; PS50934; SWIRM; 1.
PE 1: Evidence at protein level;
KW Activator; Chromatin regulator; Coiled coil; Developmental protein;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..469
FT /note="SWI/SNF complex subunit SWI3B"
FT /id="PRO_0000344528"
FT DOMAIN 48..145
FT /note="SWIRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00247"
FT DOMAIN 223..274
FT /note="SANT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00624"
FT REGION 1..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 293..314
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 360..387
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 423..447
FT /evidence="ECO:0000255"
FT COMPBIAS 15..42
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 293..310
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 368..383
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 469 AA; 52411 MW; BB4D35067B8520E1 CRC64;
MAMKAPDPGG SGEILPSTPS LSETTSGGAA AASKSAQLPS SSSDIDNIHV PSYSSWFSWT
DINDCEVRSL PEFFDSRSSS KNPKFYLYLR NSIIKQYRDD HPRKISFTDV RRTLVSDVVS
IRRVFDFLDS WGLINYNSSA SAKPLKWEEK EAGKSAGDAA SEPATTVKET AKRNCNGCKA
ICSIACFACD KYDLTLCARC YVRSNYRVGI NSSEFKRVEI SEESKPEWSD KEILLLLEAV
MHYGDDWKKV ASHVIGRTEK DCVSQFVKLP FGEQFVKESD SEDGLEMFDQ IKDSDIPESE
GIDKDGSSPN KRIKLTPLAD ASNPIMAQAA FLSALAGTNV AEAAARAAVR ALSDVDYEAD
KNASRDPNRQ DANAASSGET TRNESERAWA DAKSLIEKEE HEVEGAIKET VEVEMKKIRD
RIVHFEKLDL EMERSRKQLE EVRNLLFVDQ LNIFFHTRKA RKTEDRIEC
//
