ID   SYGB_PHEZH              Reviewed;         669 AA.
AC   B4R9A9;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 1.
DT   27-MAR-2024, entry version 70.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=PHZ_c1365;
OS   Phenylobacterium zucineum (strain HLK1).
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
OC   Caulobacteraceae; Phenylobacterium.
OX   NCBI_TaxID=450851;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HLK1;
RX   PubMed=18700039; DOI=10.1186/1471-2164-9-386;
RA   Luo Y., Xu X., Ding Z., Liu Z., Zhang B., Yan Z., Sun J., Hu S., Hu X.;
RT   "Complete genome of Phenylobacterium zucineum - a novel facultative
RT   intracellular bacterium isolated from human erythroleukemia cell line
RT   K562.";
RL   BMC Genomics 9:386-386(2008).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000747; ACG77779.1; -; Genomic_DNA.
DR   RefSeq; WP_012521923.1; NC_011144.1.
DR   AlphaFoldDB; B4R9A9; -.
DR   SMR; B4R9A9; -.
DR   STRING; 450851.PHZ_c1365; -.
DR   KEGG; pzu:PHZ_c1365; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_1_5; -.
DR   OrthoDB; 9775440at2; -.
DR   Proteomes; UP000001868; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   NCBIfam; TIGR00211; glyS; 1.
DR   PANTHER; PTHR30075:SF2; GLYCINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL 2; 1.
DR   PANTHER; PTHR30075; GLYCYL-TRNA SYNTHETASE; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   SUPFAM; SSF109604; HD-domain/PDEase-like; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..669
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101314"
SQ   SEQUENCE   669 AA;  73165 MW;  403DED50DAB7B0AC CRC64;
     MPQLLLELLS EEIPARMQAQ AARDLERLAR ERLAAEGLLP EALKTFAGPR RLTLVAEGLP
     AAQADRREEL KGPKVGAPEQ ALEGFLRKTG LTRDQLVERD GVFFATIEKP GRPTPEIVAE
     MVEAILRTFP WPKSMVSGTS KLRWVRPLRR ILCVFDGEVV PFEVDGIASG DLSEGHRFMS
     DGQPFLVKDF EGYAAGLSHR SVVLDADERK ERILEAAKTL CFARNLELVE DAGLLDEVAG
     LVEWPVPVLG DMDPAFLDLP PEVIRTSMRV HQRYFAVRDP AGGKLAPHFL TVANIAARDG
     GATIAKGNAK VLSARLSDAR FFWDEDRKVR LEDRLEKLKG VTFHAKLGTM YERVQRIEAL
     AGELAPFVRD EPETRTKAVQ AARLAKADLV SGVVGEFPEL QGIMGGYYAE AEGLDPEVVD
     AIRSHYRPQG PNDAVPVSSV AATVALADKL DTLVSFFGIG EKPTGSRDPF ALRRAALGVI
     RIVLETRTRL PLKRFVSDEV LDFFADRLAV LLREQGKRHD LVAAVFALGD DDLVRIVARV
     EVLSAFLKTE DGANLLAGYK RAVNILRAEE KKGPLPAGEP AQAAGAPAEE AALVQAVAAL
     DARLGPALER EDFEGAMTEL AKLRGPVDAF FDKVLVNSDV PAERENRLRL LAKVRDAMGR
     VADFSQVTG
//