ID   T0RZD0_SAPDV            Unreviewed;       623 AA.
AC   T0RZD0;
DT   16-OCT-2013, integrated into UniProtKB/TrEMBL.
DT   16-OCT-2013, sequence version 1.
DT   27-MAR-2024, entry version 35.
DE   RecName: Full=squalene monooxygenase {ECO:0000256|ARBA:ARBA00012312};
DE            EC=1.14.14.17 {ECO:0000256|ARBA:ARBA00012312};
GN   ORFNames=SDRG_06501 {ECO:0000313|EMBL:EQC35742.1};
OS   Saprolegnia diclina (strain VS20).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Saprolegniales; Saprolegniaceae;
OC   Saprolegnia.
OX   NCBI_TaxID=1156394 {ECO:0000313|EMBL:EQC35742.1, ECO:0000313|Proteomes:UP000030762};
RN   [1] {ECO:0000313|EMBL:EQC35742.1, ECO:0000313|Proteomes:UP000030762}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=VS20 {ECO:0000313|EMBL:EQC35742.1,
RC   ECO:0000313|Proteomes:UP000030762};
RG   The Broad Institute Genome Sequencing Platform;
RA   Russ C., Nusbaum C., Tyler B., van West P., Dieguez-Uribeondo J.,
RA   de Bruijn I., Tripathy S., Jiang R., Young S.K., Zeng Q., Gargeya S.,
RA   Fitzgerald M., Haas B., Abouelleil A., Alvarado L., Arachchi H.M.,
RA   Berlin A., Chapman S.B., Goldberg J., Griggs A., Gujja S., Hansen M.,
RA   Howarth C., Imamovic A., Larimer J., McCowen C., Montmayeur A., Murphy C.,
RA   Neiman D., Pearson M., Priest M., Roberts A., Saif S., Shea T., Sisk P.,
RA   Sykes S., Wortman J., Nusbaum C., Birren B.;
RT   "The Genome Sequence of Saprolegnia declina VS20.";
RL   Submitted (APR-2012) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000256|ARBA:ARBA00001974};
CC   -!- SIMILARITY: Belongs to the squalene monooxygenase family.
CC       {ECO:0000256|ARBA:ARBA00008802}.
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DR   EMBL; JH767149; EQC35742.1; -; Genomic_DNA.
DR   RefSeq; XP_008610504.1; XM_008612282.1.
DR   AlphaFoldDB; T0RZD0; -.
DR   STRING; 1156394.T0RZD0; -.
DR   EnsemblProtists; EQC35742; EQC35742; SDRG_06501.
DR   GeneID; 19947228; -.
DR   VEuPathDB; FungiDB:SDRG_06501; -.
DR   eggNOG; KOG1298; Eukaryota.
DR   InParanoid; T0RZD0; -.
DR   OMA; AKRTFYW; -.
DR   OrthoDB; 148348at2759; -.
DR   Proteomes; UP000030762; Unassembled WGS sequence.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR   GO; GO:0004506; F:squalene monooxygenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016126; P:sterol biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 1.
DR   InterPro; IPR006076; FAD-dep_OxRdtase.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR013698; Squalene_epoxidase.
DR   InterPro; IPR040125; Squalene_monox.
DR   PANTHER; PTHR10835; SQUALENE MONOOXYGENASE; 1.
DR   PANTHER; PTHR10835:SF0; SQUALENE MONOOXYGENASE; 1.
DR   Pfam; PF01266; DAO; 1.
DR   Pfam; PF08491; SE; 1.
DR   PRINTS; PR00420; RNGMNOXGNASE.
DR   SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
PE   3: Inferred from homology;
KW   Membrane {ECO:0000256|SAM:Phobius};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW   Reference proteome {ECO:0000313|Proteomes:UP000030762};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        34..54
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        84..107
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        127..144
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          126..156
FT                   /note="FAD dependent oxidoreductase"
FT                   /evidence="ECO:0000259|Pfam:PF01266"
FT   DOMAIN          302..576
FT                   /note="Squalene epoxidase"
FT                   /evidence="ECO:0000259|Pfam:PF08491"
SQ   SEQUENCE   623 AA;  68063 MW;  F2D35381372619AE CRC64;
     MILLIASVVI LSETLRSLLQ DAFAVLCQQL ADAPVFFSLL LAVLAACCAL RASLLQTMNT
     TSVLIIGFAA VSYRATEHDI PRPLSFVQAA GVIALVYLLG FVGMWAVKPT PKYTNDKYTE
     ANRDADVIII GGGTTGCAMA MGLAKQGKKV LVFEKTLEYQ DRFVGELMQP GGLDALRSLD
     LLDCAETETD IKAEGYTIVK TKKGTEDHIM LPYPERVPKT FSEYMGFARG DNGTGKQHGR
     GFHNGLFVDR LRAKVFAHEN VTCIEGTVTK LVSDSKTDIC RGVQYRRKAT SEDVEMPTEH
     AKAPLVLVCD GLWSGLRREL STESPKQISS FIAILMKHPP MEASVPYRGY GHVILAEPSP
     IVVYQISPTE TRALVDVPGK VPSQSNGDLT KHLLNVVAPQ MPEASRAAFV TAVQDGPVKS
     MPNREFMTTQ PSLGRCIMIG DSWNMRHPLT GGGMSVALRD AVLLTSLLEK TDLVDADAIA
     AARHAFEEQR VNHSSTINIL ANALYHVFSI PDGYSNAAAR EEIRESCYQY LKLGGMFSSG
     PLGLLCALTP KPAVLVAHFF MVAGYAASRV LTPFPTPSKL LHCYRVFHEA CLIIMPLVLR
     EDVTFLAWAP LAGLINLLFP FRR
//