ID T178A_HUMAN Reviewed; 297 AA.
AC Q8NBL3; Q6UWI6; Q8N6N4;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 24-JAN-2024, entry version 142.
DE RecName: Full=Transmembrane protein 178A;
DE Flags: Precursor;
GN Name=TMEM178A; Synonyms=TMEM178; ORFNames=PSEC0131, UNQ5926/PRO19820;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Placenta;
RX PubMed=16303743; DOI=10.1093/dnares/12.2.117;
RA Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J.,
RA Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S.,
RA Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y.,
RA Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S.,
RA Isogai T.;
RT "Signal sequence and keyword trap in silico for selection of full-length
RT human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA
RT libraries.";
RL DNA Res. 12:117-126(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT VAL-107.
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Acts as a negative regulator of osteoclast differentiation in
CC basal and inflammatory conditions by regulating TNFSF11-induced Ca (2+)
CC fluxes, thereby controlling the induction of NFATC1.
CC {ECO:0000250|UniProtKB:Q9CZ16}.
CC -!- SUBUNIT: Interacts with STIM1. {ECO:0000250|UniProtKB:Q9CZ16}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q9CZ16}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8NBL3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8NBL3-2; Sequence=VSP_025428, VSP_025429;
CC -!- SIMILARITY: Belongs to the TMEM178 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY358773; AAQ89133.1; -; mRNA.
DR EMBL; AK075440; BAC11622.1; -; mRNA.
DR EMBL; AC007246; AAX93064.1; -; Genomic_DNA.
DR EMBL; BC029530; AAH29530.1; -; mRNA.
DR CCDS; CCDS1804.1; -. [Q8NBL3-1]
DR RefSeq; NP_001161431.1; NM_001167959.1.
DR RefSeq; NP_689603.2; NM_152390.2. [Q8NBL3-1]
DR AlphaFoldDB; Q8NBL3; -.
DR BioGRID; 126251; 16.
DR IntAct; Q8NBL3; 1.
DR STRING; 9606.ENSP00000281961; -.
DR TCDB; 8.A.16.2.8; the ca(+) channel auxiliary subunit Gama1-Gama8 (ccaGama) family.
DR GlyCosmos; Q8NBL3; 1 site, No reported glycans.
DR GlyGen; Q8NBL3; 1 site.
DR PhosphoSitePlus; Q8NBL3; -.
DR BioMuta; TMEM178A; -.
DR DMDM; 74723755; -.
DR MassIVE; Q8NBL3; -.
DR PaxDb; 9606-ENSP00000281961; -.
DR PeptideAtlas; Q8NBL3; -.
DR ProteomicsDB; 72790; -. [Q8NBL3-1]
DR TopDownProteomics; Q8NBL3-1; -. [Q8NBL3-1]
DR Antibodypedia; 14741; 88 antibodies from 17 providers.
DR DNASU; 130733; -.
DR Ensembl; ENST00000281961.3; ENSP00000281961.2; ENSG00000152154.12. [Q8NBL3-1]
DR GeneID; 130733; -.
DR KEGG; hsa:130733; -.
DR MANE-Select; ENST00000281961.3; ENSP00000281961.2; NM_152390.3; NP_689603.2.
DR UCSC; uc002rrt.5; human. [Q8NBL3-1]
DR AGR; HGNC:28517; -.
DR CTD; 130733; -.
DR DisGeNET; 130733; -.
DR GeneCards; TMEM178A; -.
DR HGNC; HGNC:28517; TMEM178A.
DR HPA; ENSG00000152154; Tissue enriched (brain).
DR neXtProt; NX_Q8NBL3; -.
DR OpenTargets; ENSG00000152154; -.
DR PharmGKB; PA162405994; -.
DR VEuPathDB; HostDB:ENSG00000152154; -.
DR eggNOG; ENOG502R2WV; Eukaryota.
DR GeneTree; ENSGT00390000015299; -.
DR HOGENOM; CLU_961492_0_0_1; -.
DR InParanoid; Q8NBL3; -.
DR OMA; ATYAGLW; -.
DR OrthoDB; 5362939at2759; -.
DR PhylomeDB; Q8NBL3; -.
DR TreeFam; TF331307; -.
DR PathwayCommons; Q8NBL3; -.
DR SignaLink; Q8NBL3; -.
DR BioGRID-ORCS; 130733; 14 hits in 1151 CRISPR screens.
DR GenomeRNAi; 130733; -.
DR Pharos; Q8NBL3; Tdark.
DR PRO; PR:Q8NBL3; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; Q8NBL3; Protein.
DR Bgee; ENSG00000152154; Expressed in cerebellar hemisphere and 140 other cell types or tissues.
DR Genevisible; Q8NBL3; HS.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; ISS:UniProtKB.
DR GO; GO:0045671; P:negative regulation of osteoclast differentiation; ISS:UniProtKB.
DR GO; GO:0051480; P:regulation of cytosolic calcium ion concentration; ISS:UniProtKB.
DR Gene3D; 1.20.140.150; -; 1.
DR InterPro; IPR004031; PMP22/EMP/MP20/Claudin.
DR InterPro; IPR039625; T178A/B.
DR PANTHER; PTHR32005:SF4; TRANSMEMBRANE PROTEIN 178A; 1.
DR PANTHER; PTHR32005; TRANSMEMBRANE PROTEIN 178B-RELATED; 1.
DR Pfam; PF13903; Claudin_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Endoplasmic reticulum; Glycoprotein; Membrane;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..297
FT /note="Transmembrane protein 178A"
FT /id="PRO_0000287280"
FT TOPO_DOM 26..179
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 180..200
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 201..208
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 209..229
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 230..257
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 258..278
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 279..297
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 41..84
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 158
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 66..111
FT /note="PLRDSPPLGRRLLPGGPGRADPESWRSLLGLGGLDAECGRPLFATY -> LG
FT PLEEVLLPGHRPGHRHPHPERYCAAMHGHQVPLFSAHPLAKHSF (in isoform
FT 2)"
FT /evidence="ECO:0000303|PubMed:12975309"
FT /id="VSP_025428"
FT VAR_SEQ 112..297
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12975309"
FT /id="VSP_025429"
FT VARIANT 107
FT /note="L -> V (in dbSNP:rs17852679)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_032296"
SQ SEQUENCE 297 AA; 33019 MW; 757C5C9F7F2CFA53 CRC64;
MEPRALVTAL SLGLSLCSLG LLVTAIFTDH WYETDPRRHK ESCERSRAGA DPPDQKNRLM
PLSHLPLRDS PPLGRRLLPG GPGRADPESW RSLLGLGGLD AECGRPLFAT YSGLWRKCYF
LGIDRDIDTL ILKGIAQRCT AIKYHFSQPI RLRNIPFNLT KTIQQDEWHL LHLRRITAGF
LGMAVAVLLC GCIVATVSFF WEESLTQHVA GLLFLMTGIF CTISLCTYAA SISYDLNRLP
KLIYSLPADV EHGYSWSIFC AWCSLGFIVA AGGLCIAYPF ISRTKIAQLK SGRDSTV
//
