ID T1EE74_HELRO Unreviewed; 380 AA.
AC T1EE74;
DT 16-OCT-2013, integrated into UniProtKB/TrEMBL.
DT 16-OCT-2013, sequence version 1.
DT 27-MAR-2024, entry version 59.
DE RecName: Full=Ubiquitin carboxyl-terminal hydrolase {ECO:0000256|RuleBase:RU366025};
DE EC=3.4.19.12 {ECO:0000256|RuleBase:RU366025};
GN Name=20194876 {ECO:0000313|EnsemblMetazoa:HelroP107093};
GN ORFNames=HELRODRAFT_107093 {ECO:0000313|EMBL:ESN98961.1};
OS Helobdella robusta (Californian leech).
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Annelida; Clitellata;
OC Hirudinea; Rhynchobdellida; Glossiphoniidae; Helobdella.
OX NCBI_TaxID=6412 {ECO:0000313|EnsemblMetazoa:HelroP107093, ECO:0000313|Proteomes:UP000015101};
RN [1] {ECO:0000313|Proteomes:UP000015101}
RP NUCLEOTIDE SEQUENCE.
RA Hellsten U., Grimwood J., Chapman J.A., Shapiro H., Aerts A., Otillar R.P.,
RA Terry A.Y., Boore J.L., Simakov O., Marletaz F., Cho S.-J.,
RA Edsinger-Gonzales E., Havlak P., Kuo D.-H., Larsson T., Lv J., Arendt D.,
RA Savage R., Osoegawa K., de Jong P., Lindberg D.R., Seaver E.C.,
RA Weisblat D.A., Putnam N.H., Grigoriev I.V., Rokhsar D.S.;
RL Submitted (DEC-2012) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ESN98961.1, ECO:0000313|Proteomes:UP000015101}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=23254933; DOI=10.1038/nature11696;
RA Simakov O., Marletaz F., Cho S.J., Edsinger-Gonzales E., Havlak P.,
RA Hellsten U., Kuo D.H., Larsson T., Lv J., Arendt D., Savage R.,
RA Osoegawa K., de Jong P., Grimwood J., Chapman J.A., Shapiro H., Aerts A.,
RA Otillar R.P., Terry A.Y., Boore J.L., Grigoriev I.V., Lindberg D.R.,
RA Seaver E.C., Weisblat D.A., Putnam N.H., Rokhsar D.S.;
RT "Insights into bilaterian evolution from three spiralian genomes.";
RL Nature 493:526-531(2013).
RN [3] {ECO:0000313|EnsemblMetazoa:HelroP107093}
RP IDENTIFICATION.
RG EnsemblMetazoa;
RL Submitted (JUN-2015) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide
CC and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-
CC residue protein attached to proteins as an intracellular targeting
CC signal).; EC=3.4.19.12; Evidence={ECO:0000256|RuleBase:RU366025};
CC -!- SIMILARITY: Belongs to the peptidase C19 family.
CC {ECO:0000256|RuleBase:RU366025}.
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DR EMBL; AMQM01001083; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; KB097143; ESN98961.1; -; Genomic_DNA.
DR RefSeq; XP_009022885.1; XM_009024637.1.
DR AlphaFoldDB; T1EE74; -.
DR STRING; 6412.T1EE74; -.
DR EnsemblMetazoa; HelroT107093; HelroP107093; HelroG107093.
DR GeneID; 20194876; -.
DR KEGG; hro:HELRODRAFT_107093; -.
DR CTD; 20194876; -.
DR eggNOG; KOG1864; Eukaryota.
DR HOGENOM; CLU_008279_2_0_1; -.
DR InParanoid; T1EE74; -.
DR OMA; DNTVEEY; -.
DR OrthoDB; 227085at2759; -.
DR Proteomes; UP000015101; Unassembled WGS sequence.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0004843; F:cysteine-type deubiquitinase activity; IBA:GO_Central.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd02663; Peptidase_C19G; 1.
DR Gene3D; 3.90.70.10; Cysteine proteinases; 1.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR InterPro; IPR001394; Peptidase_C19_UCH.
DR InterPro; IPR018200; USP_CS.
DR InterPro; IPR028889; USP_dom.
DR PANTHER; PTHR24006:SF944; RE52890P; 1.
DR PANTHER; PTHR24006; UBIQUITIN CARBOXYL-TERMINAL HYDROLASE; 1.
DR Pfam; PF00443; UCH; 1.
DR SUPFAM; SSF54001; Cysteine proteinases; 1.
DR PROSITE; PS00972; USP_1; 1.
DR PROSITE; PS00973; USP_2; 1.
DR PROSITE; PS50235; USP_3; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|RuleBase:RU366025};
KW Protease {ECO:0000256|RuleBase:RU366025};
KW Reference proteome {ECO:0000313|Proteomes:UP000015101};
KW Thiol protease {ECO:0000256|RuleBase:RU366025};
KW Ubl conjugation pathway {ECO:0000256|RuleBase:RU366025}.
FT DOMAIN 41..379
FT /note="USP"
FT /evidence="ECO:0000259|PROSITE:PS50235"
SQ SEQUENCE 380 AA; 43684 MW; 63A05C855509522B CRC64;
MPEKCWSVFN LLLRRRKMGP NLSQLEKELG AGQCAINEHF IGLVNFGNTC YCNSVLQALY
FCKPFRDMVL AYKQQTKNQK RETLLTCLAD LFYTIAMQKR KVGSIPPKKF IQKLRKENGA
FDNYLQHDAH EFLNYLLNTI ADILQVEKQS QQKGSKLSST LPVLQSGSIN PSNTMPKSEP
TWIHDIFQGT FTSETRCLNC ETVRSREEDF LDLSIEVEQN TSITHCLLAF SNTETLGSEC
KYFCEVCCSK QEAQKRMRVK KLPHVLALHL KRFKLEHPNR SIKLSYRVVF PLELRLFNTS
DDASDPDRMY DLISVVIHCG SGLSRGHYIS IVKSHGLWLL FDDDIVEKIE ASTIEDFFGL
PSDLQKTSES GYILFYQSRE
//
