ID T1EEC5_HELRO Unreviewed; 817 AA.
AC T1EEC5;
DT 16-OCT-2013, integrated into UniProtKB/TrEMBL.
DT 16-OCT-2013, sequence version 1.
DT 27-MAR-2024, entry version 50.
DE RecName: Full=[heparan sulfate]-glucosamine N-sulfotransferase {ECO:0000256|ARBA:ARBA00012979};
DE EC=2.8.2.8 {ECO:0000256|ARBA:ARBA00012979};
GN Name=20194927 {ECO:0000313|EnsemblMetazoa:HelroP107720};
GN ORFNames=HELRODRAFT_107720 {ECO:0000313|EMBL:ESN94414.1};
OS Helobdella robusta (Californian leech).
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Annelida; Clitellata;
OC Hirudinea; Rhynchobdellida; Glossiphoniidae; Helobdella.
OX NCBI_TaxID=6412 {ECO:0000313|EnsemblMetazoa:HelroP107720, ECO:0000313|Proteomes:UP000015101};
RN [1] {ECO:0000313|Proteomes:UP000015101}
RP NUCLEOTIDE SEQUENCE.
RA Hellsten U., Grimwood J., Chapman J.A., Shapiro H., Aerts A., Otillar R.P.,
RA Terry A.Y., Boore J.L., Simakov O., Marletaz F., Cho S.-J.,
RA Edsinger-Gonzales E., Havlak P., Kuo D.-H., Larsson T., Lv J., Arendt D.,
RA Savage R., Osoegawa K., de Jong P., Lindberg D.R., Seaver E.C.,
RA Weisblat D.A., Putnam N.H., Grigoriev I.V., Rokhsar D.S.;
RL Submitted (DEC-2012) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ESN94414.1, ECO:0000313|Proteomes:UP000015101}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=23254933; DOI=10.1038/nature11696;
RA Simakov O., Marletaz F., Cho S.J., Edsinger-Gonzales E., Havlak P.,
RA Hellsten U., Kuo D.H., Larsson T., Lv J., Arendt D., Savage R.,
RA Osoegawa K., de Jong P., Grimwood J., Chapman J.A., Shapiro H., Aerts A.,
RA Otillar R.P., Terry A.Y., Boore J.L., Grigoriev I.V., Lindberg D.R.,
RA Seaver E.C., Weisblat D.A., Putnam N.H., Rokhsar D.S.;
RT "Insights into bilaterian evolution from three spiralian genomes.";
RL Nature 493:526-531(2013).
RN [3] {ECO:0000313|EnsemblMetazoa:HelroP107720}
RP IDENTIFICATION.
RG EnsemblMetazoa;
RL Submitted (JUN-2015) to UniProtKB.
CC -!- PATHWAY: Glycan metabolism; heparan sulfate biosynthesis.
CC {ECO:0000256|ARBA:ARBA00005093}.
CC -!- PATHWAY: Glycan metabolism; heparin biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004841}.
CC -!- SIMILARITY: Belongs to the sulfotransferase 1 family. NDST subfamily.
CC {ECO:0000256|ARBA:ARBA00010420}.
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DR EMBL; AMQM01001613; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AMQM01001614; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; KB097571; ESN94414.1; -; Genomic_DNA.
DR RefSeq; XP_009027484.1; XM_009029236.1.
DR AlphaFoldDB; T1EEC5; -.
DR STRING; 6412.T1EEC5; -.
DR EnsemblMetazoa; HelroT107720; HelroP107720; HelroG107720.
DR GeneID; 20194927; -.
DR KEGG; hro:HELRODRAFT_107720; -.
DR CTD; 20194927; -.
DR eggNOG; KOG3703; Eukaryota.
DR HOGENOM; CLU_011357_2_0_1; -.
DR InParanoid; T1EEC5; -.
DR OMA; GRQYPTM; -.
DR OrthoDB; 2913264at2759; -.
DR UniPathway; UPA00756; -.
DR UniPathway; UPA00862; -.
DR Proteomes; UP000015101; Unassembled WGS sequence.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0015016; F:[heparan sulfate]-glucosamine N-sulfotransferase activity; IBA:GO_Central.
DR GO; GO:0019213; F:deacetylase activity; IBA:GO_Central.
DR GO; GO:0015014; P:heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process; IBA:GO_Central.
DR GO; GO:0030210; P:heparin biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR021930; Heparan_SO4_deacetylase.
DR InterPro; IPR037359; NST/OST.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000863; Sulfotransferase_dom.
DR PANTHER; PTHR10605:SF56; BIFUNCTIONAL HEPARAN SULFATE N-DEACETYLASE_N-SULFOTRANSFERASE; 1.
DR PANTHER; PTHR10605; HEPARAN SULFATE SULFOTRANSFERASE; 1.
DR Pfam; PF12062; HSNSD; 1.
DR Pfam; PF00685; Sulfotransfer_1; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW Disulfide bond {ECO:0000256|PIRSR:PIRSR637359-3};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Reference proteome {ECO:0000313|Proteomes:UP000015101}.
FT DOMAIN 27..451
FT /note="Heparan sulphate-N-deacetylase"
FT /evidence="ECO:0000259|Pfam:PF12062"
FT DOMAIN 541..754
FT /note="Sulfotransferase"
FT /evidence="ECO:0000259|Pfam:PF00685"
FT ACT_SITE 550
FT /note="For sulfotransferase activity"
FT /evidence="ECO:0000256|PIRSR:PIRSR637359-1"
FT BINDING 647
FT /ligand="3'-phosphoadenylyl sulfate"
FT /ligand_id="ChEBI:CHEBI:58339"
FT /evidence="ECO:0000256|PIRSR:PIRSR637359-2"
FT BINDING 767..771
FT /ligand="3'-phosphoadenylyl sulfate"
FT /ligand_id="ChEBI:CHEBI:58339"
FT /evidence="ECO:0000256|PIRSR:PIRSR637359-2"
FT DISULFID 752..762
FT /evidence="ECO:0000256|PIRSR:PIRSR637359-3"
SQ SEQUENCE 817 AA; 95072 MW; 0DDA7D58D5E342C3 CRC64;
MNLYQCSNPI SDNFLPKYES RKTIEVKKKV LIFTDKVTNL LLKRLVYLIG SVKFNVKISH
NKLLPLLEDH QKGSFALIVF TKLDIYLHLD KHAKERLKEY CLNYKVGLLI FVQNEENIPI
TKLSEYPLFF ATNNHISNYR VNASSPVLRI TKPGYHVFNN TSSKWTLFHS KDPSFEPISV
TNCENRKAKD TCEDGLVYAA VMRDKGLHDG IEKIYFNDKI TFWVHKLIIL DAISYLSRSS
LLLPLERYIL VDIDDIFLAN SPLKMKKQDV KELVNFQNRI RKENVVENFT FNLGFSGKYY
RQGNINEMEG DEFLLASRHN FTWFDHTWSH VQPHKLNFSS LVREMLVNKK FAKIHDLPLY
TNYSVAPHHS GVYPVHDDLY EAWRQVWGLD VTSTEEYPNL YPAKNRKGFI YKGIKVLPRQ
TCNLFTHTNF FDTFPGGRTK LDNSIFGGDL FMTVITNIVT IFMTHLSNYG SDRLALYTFT
NLFNFIKCWT NLKMMTVEPS KLADVYFNFY PNEILPLWTN PCNNKRHLEI WSGQKTCKRL
PHLIVVGPQK TGTTALYTFL NLHPQIKSNF QSNKTFEEVQ FFTDKNYVNG IDWYMNHFPF
PPSESTILFE KSANYFTEAK APLQIHTLLP DTKIICILIN PSQRAYSWYQ HAQSHKDEAA
LKHSFYDVIT ATNSSSDVHN LRNKCLLPGL YTSHLNKWKT HFNNSQLLIV DGENLVEDPI
SEMEKVQNFL KLQDKLDYSK LLKYNTKKGF FCPVTNGGKL KCLGSGKGRK YSPMDDKSKD
FLKHFYKNSI EDLNDFLTNH GYSKPGWLIK EINDMNA
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