ID   T1GPL5_MEGSC            Unreviewed;       513 AA.
AC   T1GPL5;
DT   16-OCT-2013, integrated into UniProtKB/TrEMBL.
DT   16-OCT-2013, sequence version 1.
DT   27-MAR-2024, entry version 47.
DE   RecName: Full=Lipase domain-containing protein {ECO:0000259|Pfam:PF00151};
OS   Megaselia scalaris (Humpbacked fly) (Phora scalaris).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Platypezoidea;
OC   Phoridae; Megaseliini; Megaselia.
OX   NCBI_TaxID=36166 {ECO:0000313|EnsemblMetazoa:MESCA005549-PA, ECO:0000313|Proteomes:UP000015102};
RN   [1] {ECO:0000313|Proteomes:UP000015102}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=Durham, NC isolate 2 -- Noor lab
RC   {ECO:0000313|Proteomes:UP000015102};
RA   Hughes D.;
RL   Submitted (FEB-2013) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EnsemblMetazoa:MESCA005549-PA}
RP   IDENTIFICATION.
RG   EnsemblMetazoa;
RL   Submitted (JUN-2015) to UniProtKB.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC   -!- SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase family.
CC       {ECO:0000256|ARBA:ARBA00010701, ECO:0000256|RuleBase:RU004262}.
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DR   EMBL; CAQQ02390174; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; T1GPL5; -.
DR   STRING; 36166.T1GPL5; -.
DR   EnsemblMetazoa; MESCA005549-RA; MESCA005549-PA; MESCA005549.
DR   HOGENOM; CLU_027171_0_1_1; -.
DR   OMA; SPKAMYF; -.
DR   Proteomes; UP000015102; Unassembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0052689; F:carboxylic ester hydrolase activity; IEA:InterPro.
DR   GO; GO:0016298; F:lipase activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006629; P:lipid metabolic process; IEA:InterPro.
DR   CDD; cd00707; Pancreat_lipase_like; 1.
DR   Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR013818; Lipase.
DR   InterPro; IPR016272; Lipase_LIPH.
DR   InterPro; IPR033906; Lipase_N.
DR   InterPro; IPR000734; TAG_lipase.
DR   PANTHER; PTHR11610:SF184; LD47264P; 1.
DR   PANTHER; PTHR11610; LIPASE; 1.
DR   Pfam; PF00151; Lipase; 1.
DR   PIRSF; PIRSF000865; Lipoprotein_lipase_LIPH; 1.
DR   PRINTS; PR00821; TAGLIPASE.
DR   SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
PE   3: Inferred from homology;
KW   Calcium {ECO:0000256|PIRSR:PIRSR000865-2};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR000865-2};
KW   Reference proteome {ECO:0000313|Proteomes:UP000015102};
KW   Secreted {ECO:0000256|ARBA:ARBA00022525}.
FT   DOMAIN          44..347
FT                   /note="Lipase"
FT                   /evidence="ECO:0000259|Pfam:PF00151"
FT   ACT_SITE        197
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000865-1"
FT   ACT_SITE        225
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000865-1"
FT   ACT_SITE        312
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000865-1"
FT   BINDING         239
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000865-2"
FT   BINDING         241
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000865-2"
FT   BINDING         244
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000865-2"
SQ   SEQUENCE   513 AA;  57764 MW;  87F93FC5C0D243B6 CRC64;
     MYIQMANSTG ALMLQTMLYL ANQTSNSIYD LPPAQKSETT EANCYGVYGC FPINGIWLSG
     NRQVNVHPQS PSQINPQFYI FNAQNEFNKT TKIDINDVDS FRSLGINPYG KLYFMTHGFL
     EGFNPWLKEM AKTLLDYEMD KTASMIVIDW SDGSNPPYVQ AAANTRVVGA IVGHLIQTFA
     EELGMRNLDQ VHLIGHSLGS HLFGYVGFYL QRDFNRKVGR ITGLDPASPL FTGTYHEVRL
     DRTDAKFVDV IHTDANPYTS GGLGMIPRIG HVDFYPNGGY DNPGCDRQLQ EYLSKKHRPL
     IDNFQEWLGC NHVRSHKYLT ESIKNRNGFK AVTCDSYEDY RLGKCVSYED YRKLVRKGTI
     KDGDPLVAYL LTGAEPPFAR EHFKITVKVS NSEESEDHGG EVGTLTINLV TLEKKSSGPM
     KFSDSAVFLE PGMEVTRVVA GHAIKDATHA VVEWEYQTNF LNPFSWRIVE SPRIFLEYVK
     VYSIEDQKEL KLCAENGAPI IAGSPIQLKS IFC
//